Specimen disrupting method and specimen disrupting apparatus

Patent metadata

Field	Value
Publication number	US-10760115-B2
Application number	US-201515527153-A
Country	US
Kind code	B2
Filing date	Nov 17, 2015
Priority date	Nov 18, 2014
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

A specimen disrupting apparatus includes: a drive unit that rotates the lower portion of a container having a solution that includes a specimen, a great number of small diameter beads, and a large diameter bead stored therein; and a control unit that controls the drive unit. The control unit controls the drive unit such that the lower portion of the container rotates at two or more different rotational speeds which are changed continuously.

First claim

The invention claimed is:

1. A specimen disrupting apparatus comprising:
a drive unit configured to cause a lower portion of a container containing a solution which includes a specimen, a great number of small diameter beads and a large diameter bead to rotate; and
a control unit configured to control the drive unit;
the control unit controlling the drive unit such that the lower portion of the container rotates at two or more different rotational speeds which change continuously,
wherein the container comprises a lower rib in a vicinity of a bottom portion of an inner circumferential surface thereof that agitates the solution, and an upper rib at an upper portion of the inner circumferential surface thereof that causes the small diameter beads to bounce upward from the inner circumferential surface.

2. The specimen disrupting apparatus as defined in claim 1 , wherein:
the control unit controls the drive unit such that a minimum rotational speed is within a range from 1000 rpm to 5000 rpm and a maximum rotational speed is within a range from 6000 rpm to 10000 rpm, among the two or more different rotational speeds at which the lower portion of the container is rotated.

3. The specimen disrupting apparatus as defined in claim 1 , wherein:
a lower portion of the inner circumferential surface of the container is smooth such that the large diameter bead rolls on the lower inner circumferential surface during rotation.

4. The specimen disrupting apparatus as defined in claim 1 , wherein:
the driving unit comprises:
a support member configured to support the upper portion of the container in a state in which the container is not rotatable about a central axis of the container;
a rotating member that rotates around a predetermined rotational axis; and
a connector for directly or indirectly connecting the lower portion of the container to the rotating member in a state where the central axis intersects with the rotational axis.

5. The specimen disrupting apparatus as defined in claim 4 , wherein:
the angle at which the central axis intersects with the rotational axis is within a range from 2 to 5 degrees.

6. The specimen disrupting apparatus as defined in claim 4 , wherein:
the support member comprises a flexible member having a hole therein, through which the upper portion of the container is inserted.

7. The specimen disrupting apparatus as defined in claim 6 , wherein:
the flexible member comprises an annular portion, which is harder than the material of the flexible member, around the hole.

8. The specimen disrupting apparatus as defined in claim 6 , wherein:
the support member further comprises a cylindrical container housing portion having an opening communicating with the hole of the flexible member and a bottom, wherein:
a convex portion is formed on the bottom of the container housing portion; and
a projection that engages with a rib formed on the outer peripheral surface of the container to prevent rotation of the container about the central axis thereof is formed on the inner circumferential surface of the flexible member.

9. The specimen disrupting apparatus as defined in claim 4 , wherein:
the connector comprises one of a concave portion and a convex portion provided at a position remote from the rotational axis in the rotating member and one of a concave portion and a convex portion for engaging the concave portion or the convex portion, provided directly or indirectly at the lower end of the container.

10. A specimen disrupting apparatus comprising:
a plurality of drive units configured to cause a lower portion of a plurality of containers each containing a solution which includes a specimen, a great number of small diameter beads and a large diameter bead to rotate; and
a control unit configured to control the drive units;
the control unit controlling the drive units such that the lower portion of the containers rotates at two or more different rotational speeds which change continuously and
wherein the plurality of driving units rotate a plurality of the containers simultaneously, and the control unit controls the driving units such that when a portion of the containers among the plurality of containers are being rotated, the remaining containers are rotated at a different rotational speed from that of the portion of the containers.

11. A specimen disrupting method comprising:
storing a solution containing a specimen, a great number of small diameter beads, and a large diameter bead in a container; and
causing a lower portion of the container to rotate while continuously changing the rotational speed among two or more different rotational speeds, to disrupt the specimen with the beads,
wherein:
the large diameter bead is rolled on the inner circumferential surface at the lower portion of the container; and
the small diameter beads are moved in the vertical direction within the container in response to changes in the rotational speed.

12. The specimen disrupting method as defined in claim 11 , wherein:
a minimum rotational speed is within a range from 1000 rpm to 5000 rpm and a maximum rotational speed is within a range from 6000 rpm to 10000 rpm, among the two or more different rotational speeds.

13. The specimen disrupting method as defined in claim 11 , wherein:
the rotation is performed in a state where the central axis of the container intersects with the rotational axis about which the lower portion of the container rotates.

14. The specimen disrupting method as defined in claim 13 , wherein:
the rotation is performed in a state in which the upper portion of the container is being supported.

15. The specimen disrupting method as defined in claim 13 , wherein:
the angle at which the central axis intersects with the rotational axis is within a range from 2 to 5 degrees.

16. The specimen disrupting method as defined in claim 11 , wherein:
a plurality of additional containers are prepared;
the solution, the great number of small diameter beads, and the large diameter bead is stored in each of the additional containers; and
the plurality of additional containers are rotated simultaneously.

17. The specimen disrupting method as defined in claim 16 , wherein:
the plurality of containers, including the container and the additional containers, are divided into two groups; and
while the lower portions of the containers which belong to a first group are being rotated, the lower portions of the containers which belong to a second group are rotated at a rotational speed different from that of the first group.

Assignees

• Fujifilm Wako Pure Chemical Corp
• Fujifilm Techno Products Co Ltd

Inventors

• Yoneda Akito
• Kawabata Tomohisa
• Endo Yoichi
• Hibe Daisuke
• Tsuyuki Mikio
• Denawa Tatsuyuki

Classifications

• G01N1/286 Primary

  involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F) · CPC title

• B01F33/251

  using balls as loose mixing element · CPC title

• B01F29/321

  of test-tubes or the like · CPC title

• C12M1/00

  Apparatus for enzymology or microbiology · CPC title

• G01N1/28

  Preparing specimens for investigation {including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q}(mounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 {; laboratory gas handling apparatus B01L5/00}) · CPC title