Materials and methods for increasing the tocopherol content in seed oil

Patent metadata

Field	Value
Publication number	US-10760089-B2
Application number	US-201515525768-A
Country	US
Kind code	B2
Filing date	Nov 13, 2015
Priority date	Nov 14, 2014
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

The present invention relates generally to the field of molecular biology and concerns increasing the tocopherol content of a plant relative to a control plant, comprising expressing in a plant at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase. The present invention also relates to methods for the manufacture of oil, fatty acid- or lipids-containing compositions, and to such oils and lipids as such.

First claim

The invention claimed is:

1. A method for increasing total tocopherol content of a plant relative to a control plant, comprising:
(a) providing a transgenic plant transformed with multiple expression cassettes comprising transgenes which comprise at least one polynucleotide encoding a delta-12-desaturase from Phythophthora species, at least one polynucleotide encoding a delta-6-desaturase from Ostreococcus species, at least one polynucleotide encoding a delta-6-elongase from Physcomitrella species or Thalassiosira species, and at least one polynucleotide encoding a delta-5-desaturase from Thraustochytrium species;
(b) obtaining transgenic seed oil from the transgenic plant; and
(c) measuring total tocopherol content in said transgenic seed oil,
wherein said transgenic seed oil has increased total tocopherol content as compared to a control plant of the same species, wherein the transgenic plant is an oilseed plant of the genus Brassica , and wherein expression of said transgenes in said transgenic plant results in said increase in total tocopherol content in said transgenic seed oil.

2. The method according to claim 1 , wherein said multiple expression cassettes further comprise a transgene which comprises at least one polynucleotide encoding an omega-3-desaturase from Phythophthora species or Pythium species, and wherein said omega-3-desaturase is expressed in said transgenic plant.

3. The method according to claim 1 , wherein said multiple expression cassettes further comprise a transgene which comprises at least one polynucleotide encoding a delta-5-elongase from Ostreococcus species, and wherein said delta-5-elongase is expressed in said transgenic plant.

4. The method according to claim 1 , wherein said multiple expression cassettes further comprises a transgene which comprises at least one polynucleotide encoding a delta-4-desaturase from Thraustochytrium species or Pavlova species, and wherein said delta-4-desaturase is expressed in said transgenic plant.

5. The method of claim 1 , wherein said delta-6 elongase is from Physcomitrella patens or Thalassiosira pseudonana ; said delta-12 desaturase is from Phythophthora sojae ; and said delta-6 desaturase is from Ostreococcus tauri.

6. The method of claim 1 , wherein said polynucleotides are present on one T-DNA or recombinant DNA construct having said multiple expression cassettes which are stably integrated in the genome of the transgenic plant.

7. The method of claim 2 , wherein said omega-3-desaturase is from Phythophthora infestans or Pythium irregular.

8. The method of claim 3 , wherein said delta-5 elongase is from Ostreococcus tauri.

9. The method of claim 4 , wherein said delta-4 desaturase is from Thraustochytrium species or Pavlova lutheri.

10. The method according to claim 1 , wherein said multiple expression cassettes further comprise a transgene which comprises at least one polynucleotide encoding an omega-3-desaturase from Phythophthora species or Pythium species, a transgene which comprises at least one polynucleotide encoding a delta-5-elongase from Ostreococcus species, and a transgene which comprises at least one polynucleotide encoding a delta-4-desaturase from Thraustochytrium species or Pavlova species.

11. The method of claim 10 , wherein said delta-6 elongase is from Physcomitrella patens or Thalassiosira pseudonana ; said delta-12 desaturase is from Phythophthora sojae ; said delta-6 desaturase is from Ostreococcus tauri ; said omega-3 desaturase is from Pythium irregular or Phythophthora infestans ; said delta-5 elongase is from Ostreococcus tauri ; and said delta-4 desaturase is from Thraustochytrium species or Pavlova lutheri.

Assignees

• Basf Plant Science Co Gmbh

Inventors

• Andre Carl

Classifications

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• A61K36/31

  Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi · CPC title

• A23K20/158 Primary

  Fatty acids; Fats; Products containing oils or fats · CPC title

• C12Y114/19

  with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19) · CPC title

• C12Y602/01003

  Long-chain-fatty-acid-CoA ligase (6.2.1.3) · CPC title