Isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency

Patent metadata

Field	Value
Publication number	US-10760088-B2
Application number	US-201214115397-A
Country	US
Kind code	B2
Filing date	May 2, 2012
Priority date	May 3, 2011
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

Provided are isolated polynucleotides encoding a polypeptide at least 80% homologous to the amino acid sequence selected from the group consisting of SEQ ID NOs: 757, 456-756, 758-774, 8385-10836, and 10838-14462; and isolated polynucleotide comprising nucleic acid sequences at least 80% identical to SEQ ID NO: 377, 1-376, 378-455, and 775-8384. Also provided are nucleic acid constructs comprising same, isolated polypeptides encoded thereby, transgenic cells and transgenic plants comprising same and methods of using same for increasing yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality, abiotic stress tolerance, and/or nitrogen use efficiency of a plant.

First claim

What is claimed is:

1. A method of increasing vegetative growth and reducing time to flowering of a plant, comprising:
(a) transforming a cell of the plant with a nucleic acid construct comprising a polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 80% sequence identity and conservative amino acid substitutions with respect to SEQ ID NO: 600, to thereby over-express said polypeptide within the plant as compared to a native plant of the same species under the same growth conditions, and;
(b) measuring the vegetative growth and the time to flowering of said plant over-expressing said polypeptide, wherein said measuring vegetative growth comprises measuring leaf blade area, plot coverage, rosette area, rosette diameter, and/or dry weight,
(c) selecting plants over-expressing said polypeptide for an increased vegetative growth and for a decreased time to flowering under non-stress conditions as compared to said vegetative growth and said time to flowering of a native plant of the same species under the same growth conditions, and;
(d) isolating a regenerable portion of said plants selected according to step (c) having said increased vegetative growth and said decreased time to flowering under non-stress conditions as compared to said native plant under the same growth conditions so as to obtain an isolated regenerable portion of said selected plants; and
(e) regenerating plants from said isolated regenerable portion of said selected plants, wherein plants without the said increased vegetative growth and said decreased time to flowering are not isolated and not regenerated,
thereby increasing the vegetative growth and reducing the time to flowering of the plant.

2. The method of claim 1 , wherein said polypeptide is set forth by SEQ ID NO: 600.

3. The method of claim 1 , wherein said polynucleotide is operably linked to a heterologous promoter, and wherein said polynucleotide comprises a nucleic acid sequence at least 98% identical to SEQ ID NO: 399.

4. The method of claim 1 , wherein said polynucleotide is operably linked to a heterologous promoter, and wherein said polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO: 399.

5. The method of claim 1 , wherein said vegetative growth is measured by plot coverage
thereby increasing the plot coverage and reducing the time to flowering of said plant.

6. The method of claim 5 , wherein said polynucleotide is operably linked to a heterologous promoter, and wherein said polynucleotide comprises a nucleic acid sequence at least 98% identical to SEQ ID NO: 399.

7. The method of claim 5 , wherein said polynucleotide is operably linked to a heterologous promoter, and wherein said polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO: 399.

8. The method of claim 5 , wherein said polypeptide is set forth by SEQ ID NO: 600.

9. The method of claim 1 , wherein said vegetative growth is measured by leaf blade area,
thereby generating said plant with the increased leaf blade area and the reduced time to flowering as compared to the native plant of the same species under the same growth conditions.

10. The method of claim 9 , wherein said polynucleotide is operably linked to a heterologous promoter, and wherein said polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO: 399.

11. The method of claim 9 , wherein said polypeptide is set forth by SEQ ID NO: 600.

12. The method of claim 1 , wherein said amino acid sequence has at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 600.

13. The method of claim 1 , wherein said amino acid sequence has at least 98% sequence identity to the amino acid sequence set forth in SEQ ID NO: 600.

14. The method of claim 1 , wherein said vegetative growth is measured by rosette area,
thereby generating said plant with the increased rosette area and the reduced time to flowering as compared to the native plant of the same species under the same growth conditions.

15. A method of selecting a plant, comprising:
(a) providing plants transformed with a nucleic acid construct comprising a polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 80% sequence identity and conservative amino acid substitutions with respect to SEQ ID NO: 600; and
(b) selecting said plants of step (a) for a reduced time to flowering and for an increased vegetative growth under non-stress conditions as compared to the time to flowering and the vegetative growth of a native plant of the same species under the same growth conditions, wherein said increased vegetative growth comprises increased leaf blade area, plot coverage, rosette area, rosette diameter, and/or dry weight,
thereby selecting the plant.

16. The method of claim 15 , wherein said amino acid sequence has at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 600.

17. The method of claim 15 , wherein said amino acid sequence has at least 98% sequence identity to the amino acid sequence set forth in SEQ ID NO: 600.

18. The method of claim 15 , wherein said polypeptide is set forth by SEQ ID NO: 600.

19. The method of claim 15 , wherein said polynucleotide is operably linked to a heterologous promoter, and wherein said polynucleotide comprises a nucleic acid sequence at least 98% identical to SEQ ID NO: 399.

20. The method of claim 15 , wherein said polynucleotide is operably linked to a heterologous promoter, and wherein said polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO: 399.

21. A method of selecting a plant, comprising: (a) providing plants transformed with a nucleic acid construct comprising a polynucleotide encoding a polypeptide comprising an amino acid sequence having at least 95% sequence identity and conservative amino acid substitutions with respect to SEQ ID NO: 600; and (b) selecting said plants of step (a) for a reduced time to flowering or for an increased vegetative growth under non-stress conditions as compared to the time to flowering or the vegetative growth of a native plant of the same species under the same growth conditions, wherein said increased vegetative growth comprises increased leaf blade area, plot coverage, rosette area, rosette diameter, and/or dry weight, thereby selecting the plant.

22. The method of claim 21 , wherein said amino acid sequence has at least 98% sequence identity to the amino acid sequence set forth in SEQ ID NO: 600.

23. The method of claim 21 , wherein said polypeptide is set forth by SEQ ID NO: 600.

24. The method of claim 21 , wherein said polynucleotide is operably linked to a heterologous promoter, and wherein said polynucleotide comprises a nucleic acid sequence at least 98% identical to SEQ ID NO: 399.

25. The method of claim 21 , wherein said polynucleotide is operably linked to a heterologous promoter, and wherein said polynucleotide comprises the nucleic acid sequence set forth by SEQ ID NO: 399.

Assignees

• Matarasso Noa
• Emmanuel Eyal
• Panik David
• Dangoor Inbal Nurith
• Karchi Hagai
• Evogene Ltd

Inventors

• Matarasso Noa
• Emmanuel Eyal
• Panik David
• Dangoor Inbal Nurith
• Karchi Hagai

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C07K14/415

  from plants · CPC title

• C12N15/8271

  for stress resistance, e.g. heavy metal resistance · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8246

  Non-starch polysaccharides, e.g. cellulose, fructans, levans · CPC title