Sulfite compositions

Patent metadata

Field	Value
Publication number	US-10760037-B2
Application number	US-201615741519-A
Country	US
Kind code	B2
Filing date	Jul 1, 2016
Priority date	Jul 3, 2015
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

The present invention relates to a composition comprising: (a) a source of sulfite; and (b) a lipase variant of a parent lipase, wherein said variant has improved stability towards sulfite as compared to the parent lipase.

First claim

The invention claimed is:

1. A composition comprising: (a) a source of sulfite; and (b) a lipase variant of a parent lipase,
wherein the variant has lipase activity;
wherein the variant has improved stability in presence of the source of sulfite as compared to the parent lipase;
wherein the parent lipase has the amino acid sequence of SEQ ID NO: 2; SEQ ID NO: 4; SEQ ID NO: 6; SEQ ID NO: 8; SEQ ID NO: 10; or SEQ ID NO: 12; and
wherein the variant is a polypeptide having at least 80%, but less than 100% sequence identity to SEQ ID NO: 2; SEQ ID NO: 4; SEQ ID NO: 6; SEQ ID NO: 8; SEQ ID NO: 10; or SEQ ID NO: 12; and wherein the lipase variant comprises a set of amino acid substitutions selected from the group consisting of:

N33K F51V E96V K98E E210K L259F;

N33K F51V S85K K98E;

N33K F51V K98E E210V;

P250V;

N33K F51V E87T K98E E210K I252W L259F;

N33K F51V L93K K98E;

S83T I86P G91A N92D E96L L97P K98Q;

N33K F51V N92K K98E;

N33K A40E F51V K98E;

N33K F51V E87V K98E;

Q15R N33K F51V K98E;

N33K F51V E87L K98E;

N33K F51V K98E L264P;

N33K A40Q F51V K98E;

N33K F51V K98E L264W;

E210R;

N33K F51V E56K L69R K98E I196V;

Q4K N33K F51V K98E;

N33K F51V G91T K98E;

N33K A38L F51V K98E;

V2K N33K F51V E56K L69R K98E V230R;

N33K F51V G91V K98E;

N33K F51V E56K L69R E96T K98E;

N33K F51V K98E T189D;

N33K F51V K98E R125Q;

N33K A40I F51V K98E;

N33K F51V K98E K223T;

R27D A38G E96D A111D K163G S254D T256P;

N33K F51V K98E R133Q;

N33K F51V L69C K98E;

N33K F51V G91A K98E;

N33K F51V G91F K98E;

N33K F51V K98E S216P;

N33K A40G F51V K98E;

N33K F51V K98E N251G;

N33K F51V G91Y K98E;

N33K F51V K98E V154L;

G23S N33K F51V K98E;

N33K F51V K98E R108K;

E1H N33K F51V K98E;

N33K F51V K98E S170T;

G23T N33K F51V K98E;

N33K F51V K98E T267V;

N33K F51V K98E H135Y;

N33K F51V K98E L269D;

F51V E87K K98E E210Q L227G;

N33K F51V K98E T267L;

N33K F51V K98E R139M;

R27D N33K F51V K98E;

N33K F51V K98E N162G;

N33K E210R;

N33K F51V L69R E96V K98E E210K F211W;

N33K F51V K98E N251G;

R27D N33K F51V K98E;

G23H N33K F51V K98E;

N33K F51V E96T K98E E210K;

N33K E87K;

N33K F51V E96D K98E E210K;

N33K F51V K98E L264E;

N33K F51V L69R E96V K98E E210K F211W;

N33K F51V E56K K98E K163P V176L E210K Y220F L227G;

F51V E210K;

V2K N33K F51V L227G;

N33K F51V K98I;

N33K F51V K98E E210K F211W L259F;

F51V E56K L69R G91A N92D E96L K98Q;

N33K F51V E56K L69R K98E I196V;

F51V E96T K98E E210K;

N33K F51V K98E E210K F211W I252W;

F51V E87K K98I;

N33K F51V K98E N101D K163P E210K;

F51V N101D E210K;

F51V E96D K98E E210K;

N33K F51V K98E V176L E210K L227G;

N33K F51V E96T K98E E210K F211W;

N33K F51V E56K L69R E96D K98E;

N33K F51V E56K L69R K98E T267L;

N33K F51V K98E N101D;

N33K F51V E56K L69R K98E A180D;

N33K F51V E56K L69R E96D K98E; and

N33K F51V E56K L69R K98E D165N.

Assignees

• Novozymes As

Inventors

• Malten Marco
• Poulsen Thomas Agersten
• Mikkelsen Lise Munch
• Klitgaard Dorte Marie Koefoed
• Nielsen Vibeke Skovgaard
• Larson Katarina

Classifications

• C11D3/122

  Sulfur-containing, e.g. sulfates, sulfites or gypsum · CPC title

• C11D3/201

  linear · CPC title

• C11D3/38627 Primary

  containing lipase · CPC title

• C11D1/83 Primary

  Mixtures of non-ionic with anionic compounds · CPC title

• C12N9/20

  Triglyceride splitting, e.g. by means of lipase · CPC title