Hybridoma cell strain secreting nifursolol residue marker monoclonal antibody

Patent metadata

Field	Value
Publication number	US-10759845-B2
Application number	US-201816221719-A
Country	US
Kind code	B2
Filing date	Dec 17, 2018
Priority date	Aug 9, 2018
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody with CGMCC No. 18525 is prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 μg/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.

First claim

What is claimed is:

1. A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody, preserved at the China General Microbiological Culture Collection Center (Address: No. 3, #1 Yard, Beichen West Road, Chaoyang District, Beijing) on Oct. 14, 2019 with CGMCC No. 18525.

2. A nifursol residue marker monoclonal antibody which is secreted by the hybridoma cell strain of claim 1 .

Assignees

• Univ Jiangnan

Inventors

• Kuang Hua
• Xu Chuanlai
• Wu Aihong
• Xu Liguang
• Ma Wei
• Liu Liqiang
• Wu Xiaoling
• Song Shanshan
• Hu Yongming

Classifications

• C07K16/44 Primary

  against material not provided for elsewhere {, e.g. haptens, metals, DNA, RNA, amino acids} · CPC title

• C07K16/065 Primary

  Purification, fragmentation · CPC title

• G01N33/577

  involving monoclonal antibodies {binding reaction mechanisms characterised by the use of monoclonal antibodies (G01N33/5302 - G01N33/576 take precedence)} · CPC title

• C07K14/795

  Porphyrin- or corrin-ring-containing peptides · CPC title

• C07K16/1289

  Mycobacteriaceae (F) · CPC title