Cancer antigen helper peptide

Patent metadata

Field	Value
Publication number	US-10759840-B2
Application number	US-201715831484-A
Country	US
Kind code	B2
Filing date	Dec 5, 2017
Priority date	Apr 23, 2009
Publication date	Sep 1, 2020
Grant date	Sep 1, 2020

Abstract

The present invention relates to a WT1 peptide which has an amino acid sequence consisting of contiguous amino acids derived from a WT1 protein and induces WT1-specific helper T cells by binding to an MHC class II molecule, a pharmaceutical composition comprising them and the like.

First claim

The invention claimed is:

1. A peptide that induces WT1-specific helper T cells by binding to an MHC class II molecule, wherein the peptide consists of an amino acid sequence in which one to three amino acids are substituted, deleted, or added in the amino acid sequences depicted in SEQ ID NO:4.

2. The peptide according to claim 1 , wherein the MHC class II molecule is selected from the group consisting of DRB1*0101, DRB1*0405, DRB1*0802, DRB1*0803, DRB1*0901, DRB1*1201, DRB1*1403, DRB1*1501, DRB1*1502, DPB1*0201, DPB1*0202, DPB1*0402, DPB1*0501 DPB1*0901, DQB1*0301, DQB1*0302, DQB1*0401, DQB1*0501, DQB1*0601, DQB1*0602, and DRB5*0102.

3. The peptide according to claim 1 , wherein the MHC class II molecule is selected from the group consisting of DRB1*0101, DRB1*0405, DRB1*1502, DPB1*0201, DPB1*0202, and DQB1*0601.

4. A pharmaceutical composition for treating or preventing cancer, comprising the peptide according to claim 1 .

5. A method for treating or preventing cancer, which comprises administering an effective amount of the peptide according to claim 1 to a subject having an MHC class II molecule selected from the group consisting of DRB1*0101, DRB1*0405, DRB1*0802, DRB1*0803, DRB1*0901, DRB11201, DRB1*1403, DRB1*1501, DRB1*1502, DPB1*0201, DPB1*0202, DPB1*0402, DPB1*0501, DPB1*0901, DQB1*0301, DQB1*0302, DQB1*0401, DQB1*0501, DQB1*0601, DQB1*0602, and DRB5*0102.

6. A method for inducing antigen presenting cells, which comprises culturing immature antigen presenting cells in the presence of the peptide according to claim 1 , and inducing antigen presenting cells, which display the peptide through an MHC class II molecule selected from the group consisting of DRB1*0101, DRB1*0405, DRB1*0802, DRB1*0803, DRB1*0901, DRB1*1201, DRB1*1403, DRB1*1501, DRB1*1502, DPB1*0201, DPB1*0202, DPB1*0402, DPB1*0501, DPB1*0901, DQB1*0301, DQB1*0302, DQB1*0401, DQB1*0501, DQB1*0601, DQB1*0602, and DRB5*0102, from the immature antigen presenting cells.

7. A method for inducing WT1-specific helper T cells, which comprises culturing peripheral blood mononuclear cells in the presence of the peptide according to claim 1 , and inducing WT1-specific helper T cells from the peripheral blood mononuclear cells.

8. A kit for inducing WT1-specific helper T cells, comprising, as an essential ingredient, the peptide according to claim 1 .

9. A kit for preventing or treating cancer, comprising, as an essential ingredient the peptide according to claim 1 .

10. A method for determining the presence or amount of WT1-specific helper T cells in a subject having an MHC class II molecule which is selected from the group consisting of DRB1*0101, DRB1*0405, DRB1*0802, DRB1*0803, DRB1*0901, DRB1*1201, DRB1*1403, DRB1*1501, DRB1*1502, DPB1*0201, DPB1*0202, DPB1*0402, DPB1*0501, DPB1*0901, DQB1*0301, DQB1*0302, DQB1*0401, DQB1*0501, DQB1*0601, DQB1*0602, and DRB5*0102, wherein said method comprises the steps of:
(a) reacting the peptide according to claim 1 with a sample derived from the subject; and
(b) determining the presence or amount of a cytokine contained in the sample.

Assignees

• Int Inst Cancer Immunology Inc

Inventors

• Sugiyama Haruo

Classifications

• G01N33/57525

  of the liver or pancreas · CPC title

• C12N5/0634

  Cells from the blood or the immune system · CPC title

• C12N5/0635

  B lymphocytes · CPC title

• C12N5/0639

  Dendritic cells, e.g. Langherhans cells in the epidermis · CPC title

• C12N5/0636

  T lymphocytes · CPC title