Chromatography ligand

Patent metadata

Field	Value
Publication number	US-10751645-B2
Application number	US-201815882998-A
Country	US
Kind code	B2
Filing date	Jan 29, 2018
Priority date	Oct 21, 2004
Publication date	Aug 25, 2020
Grant date	Aug 25, 2020

Abstract

The present invention relates to a chromatography ligand defined by the following formula R 1 —R 2 —N(R 3 )—R 4 —R 5 wherein R 1 is a substituted or non-substituted phenyl group; R 2 is a hydrocarbon chain comprising 0-4 carbon atoms; R 3 is a hydrocarbon chain comprising 1-3 carbon atoms; R 4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R 5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

First claim

What is claimed is:

1. A separation matrix comprising a chromatography ligand coupled to a support wherein the ligand is defined by the following formula:
R 1 —R 2 —N(R 3 )—R 4 —R 5

wherein
R 1 is a non-substituted phenyl group;
R 2 is methylene;
R 3 is a straight saturated hydrocarbon chain comprising 1-3 carbon atoms;
R 4 is a straight saturated hydrocarbon chain comprising 1-5 carbon atoms; and
R 5 is OH or H,

wherein said chromatography ligand is coupled to a support via the tertiary amine nitrogen atom through a linker such that the tertiary amine of the ligand is converted to a quaternary amine.

2. The matrix of claim 1 , wherein R 4 is CH 2 —CH 2 .

3. The matrix of claim 1 , wherein R 3 is —CH 3 .

4. The matrix of claim 1 , wherein R 4 is —CH 2 —CH 2 —CH 2 —.

5. The matrix of claim 1 , wherein the ligand comprises N-benzyl-N-methyl ethanol amine.

6. The matrix of claim 1 , wherein the ligand comprises N,N-dimethyl benzyl amine.

7. The matrix of claim 1 , wherein the support is porous agarose.

8. The matrix of claim 1 , wherein the support comprises substantially spherical particles.

9. The matrix of claim 1 , wherein the matrix is packed in a chromatography column.

10. The matrix of claim 1 , wherein the support comprises a membranous structure.

11. A separation matrix comprising a chromatography ligand coupled to a support wherein the ligand is defined by the following formula:
R 1 —R 2 —N(R 3 )—R 4 —H

wherein
R 1 is a non-substituted phenyl group;
R 2 is a methylene;
R 3 is a straight saturated hydrocarbon chain comprising 1-3 carbon atoms; and
R 4 is a straight saturated hydrocarbon chain comprising 1-5 carbon atoms,

wherein said chromatography ligand is coupled to a support via the tertiary amine nitrogen atom through a linker such that the tertiary amine of the ligand is converted to a quaternary amine.

12. The matrix of claim 11 , wherein R 4 is —CH 2 —CH 2 —.

13. The matrix of claim 11 , wherein R 3 is —CH 3 .

14. The matrix of claim 11 , wherein R 4 is —CH 2 —CH 2 —CH 2 —.

15. The matrix of claim 11 , wherein the ligand comprises N,N-dimethyl benzyl amine.

16. The matrix of claim 11 , wherein the support is porous agarose.

17. The matrix of claim 11 , wherein the support comprises substantially spherical particles.

18. The matrix of claim 11 , wherein the matrix is packed in a chromatography column.

19. The matrix of claim 11 , wherein the support comprises a filter.

Assignees

• Ge Healthcare Bioprocess R&D Ab

Inventors

• Engstrand Carina
• Forss Annika
• Glad Gunnar
• Johansson Bo-Lennart
• Johansson Hans J
• Maloisel Jean-Luc

Classifications

• B01J41/04 Primary

  Processes using organic exchangers · CPC title

• B01D15/32

  Bonded phase chromatography · CPC title

• B01D15/36

  involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion · CPC title

• C07K16/00

  Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies · CPC title

• B01D15/3847

  Multimodal interactions · CPC title