Method for simultaneous multicomponent analysis using mass spectrometry and mass spectrometer

Patent metadata

Field	Value
Publication number	US-10748751-B2
Application number	US-202016816541-A
Country	US
Kind code	B2
Filing date	Mar 12, 2020
Priority date	Jan 18, 2018
Publication date	Aug 18, 2020
Grant date	Aug 18, 2020

Abstract

In a simultaneous multicomponent analysis for a number of target compounds, an MRM transition which does not give the highest signal intensity but gives a lower signal intensity is selected for a compound having a high measurement sensitivity or a compound having a high measurement target concentration. If the signal intensity is still high, the level of collision energy (CE) is changed from an optimum level. The MRM transition, CE level and other measurement conditions determined for each compound in this manner are stored in a compound-related information storage 41 . In the process of preparing a control sequence for the simultaneous multicomponent analysis, the measurement conditions stored in the storage section 41 are used. The use of those conditions prevents the saturation of the signal for a high-concentration compound while ensuring a sufficiently high level of sensitivity for a low-concentration compound.

First claim

The invention claimed is:

1. A method for a simultaneous multicomponent analysis in which an SIM (selected ion monitoring) measurement or MRM (multiple reaction monitoring) measurement is performed for each of a plurality of known target compounds in a sample using a mass spectrometer, and a quantity of each of the compounds is determined based on a result of the measurement, wherein:
a mass-resolving power, which is one of measurement conditions, is set at a higher level in a measurement for a target compound having a comparatively high measurement target concentration or comparatively high measurement sensitivity, than in a measurement for a target compound having a comparatively low measurement target concentration or comparatively low measurement sensitivity.

2. A tandem mass spectrometer used for the method for a simultaneous multicomponent analysis according to claim 1 , comprising:
a compound-related information storage section for storing, for each of all target compounds, an MRM transition to be monitored in an MRM measurement as well as the mass-resolving power or a parameter which determines the mass-resolving power; and
a control sequence creator for creating a control sequence used for performing a simultaneous multicomponent analysis, using information stored in the compound-related information storage section,
where the information stored in the compound-related information storage section is prepared in such a manner that a value of either the mass-resolving power or the parameter which determines the mass-resolving power which yields a measurement sensitivity lower than a highest measurement sensitivity is stored for a target compound having a comparatively high measurement target concentration or comparatively high measurement sensitivity.

Assignees

• Shimadzu Corp

Inventors

• Kitano Riki

Classifications

• H01J49/4215

  Quadrupole mass filters (H01J49/4225 takes precedence) · CPC title

• H01J49/0027 Primary

  Methods for using particle spectrometers · CPC title

• H01J49/004

  Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn · CPC title

• H01J49/009

  Spectrometers having multiple channels, parallel analysis · CPC title

• H01J49/005

  by collision with gas, e.g. by introducing gas or by accelerating ions with an electric field · CPC title