Apparatuses, systems and methods for sequencing using capillary electrophoresis

Patent metadata

Field	Value
Publication number	US-10746695-B2
Application number	US-201515124129-A
Country	US
Kind code	B2
Filing date	Mar 6, 2015
Priority date	Mar 7, 2014
Publication date	Aug 18, 2020
Grant date	Aug 18, 2020

Abstract

In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. In various embodiments the reservoir can contain a separation matrix. In various embodiments the pump can load a capillary with separation matrix. In another aspect the biological sequencing device can include one or more capillaries and an integrated valve assembly. In various embodiments the integrated valve assembly can provide a polymer to the one or more capillaries.

First claim

What is claimed is:

1. A biological sequencing device, comprising:
a removable cartridge containing: a capillary tube, a pump, an actuator, a reservoir, and an optical access area;
wherein the reservoir includes a separation matrix, and wherein the pump and actuator are integral parts of the cartridge and are configured to supply the separation matrix to the capillary tube; and
wherein the removable cartridge forms a single temperature control zone where the capillary tube, the pump, the actuator, the reservoir and the optical access area are all substantially enclosed in the removable cartridge.

2. The biological sequencing device of claim 1 , wherein the separation matrix comprises a polymer and a buffer.

3. The biological sequencing device of claim 1 , wherein the removable cartridge further comprises an anode and a cathode configured to provide high voltage to the capillary tube.

4. The biological sequencing device of claim 1 , wherein the removable cartridge is heated.

5. The biological sequencing device of claim 1 , wherein the reservoir is capable of containing a quantity of separation matrix for more than one sequencing run.

6. The biological sequencing device of claim 1 , further comprising an optical detection system.

7. The biological sequencing device of claim 5 , wherein the reservoir is capable of containing a quantity of separation matrix for 100 sequencing runs.

8. A removable biological sequencing cartridge for a sequencing instrument, comprising:
a capillary tube;
a pump;
an actuator;
an optical access area; and
a reservoir, wherein the reservoir includes a separation matrix, wherein the pump and actuator are integral parts of the cartridge and are configured to supply the separation matrix to the capillary tube, and wherein the removable cartridge forms a single temperature control zone where the capillary tube, the pump, the actuator, the optical access area and the reservoir are all substantially enclosed in the removable cartridge.

9. The removable biological sequencing cartridge of claim 8 , wherein the separation matrix comprises a polymer and a buffer.

10. The removable biological sequencing cartridge of claim 8 , wherein the removable cartridge further comprises an anode and a cathode configured to provide high voltage to the capillary tube.

11. The removable biological sequencing cartridge of claim 8 , wherein the removable cartridge is heated.

12. The removable biological sequencing cartridge of claim 8 , wherein the reservoir is capable of containing a quantity of separation matrix for more than one sequencing run.

13. The removable biological sequencing cartridge of claim 12 , wherein the reservoir is capable of containing a quantity of separation matrix for 100 sequencing runs.

14. The removable biological sequencing cartridge of claim 8 , wherein the sequencing instrument includes an optical detection system.

Assignees

• Life Technologies Corp

Inventors

• Walton Ian
• Cobene Robert
• Dukhovny Alexander
• Sannicandro Adam
• Dixon John
• Karger Achim
• Kline Dan

Classifications

• G01N27/44743 Primary

  Introducing samples · CPC title

• G01N27/44708

  Cooling · CPC title

• G01N35/10

  Devices for transferring samples {or any liquids} to, in, or from, the analysis apparatus, e.g. suction devices, injection devices {(G01N35/0099 takes precedence)} · CPC title

• G01N27/44756 Primary

  Apparatus specially adapted therefor · CPC title

• G01N35/1016

  Control of the volume dispensed or introduced · CPC title