Production method for tagatose

Patent metadata

Field	Value
Publication number	US-10745720-B2
Application number	US-201414892710-A
Country	US
Kind code	B2
Filing date	Jun 3, 2014
Priority date	Jun 5, 2013
Publication date	Aug 18, 2020
Grant date	Aug 18, 2020

Abstract

A method for producing tagatose comprises: a) performing epimerization of fructose using hexuronate C4-epimerase to obtain an epimerized product comprising tagatose; b) purifying the epimerized product; and c) crystallizing the purified epimerized product. The hexuronate C4-epimerase is an enzyme derived from Thermotoga maritima, Thermotoga neapolitana, Thermotoga thermarum or mutants thereof. The hexuronate C4-epimerase is produced from strains Escherichia coli, Corynebacterum glutamicum, Aspergillus oryzae , or Bacillus subtilis.

First claim

The invention claimed is:

1. A method for producing tagatose, comprising:
a) performing epimerization of fructose using an enzyme to obtain an epimerized product comprising tagatose, wherein the enzyme comprises the amino acid sequence of SEQ ID NO: 4 or an amino acid sequence encoded by the nucleic acid sequence of SEQ ID NO: 1, 2 or 3; and
b) purifying the epimerized product.

2. The method for producing tagatose according to claim 1 , wherein the enzyme is derived from Thermotoga maritima or Thermotoga neapolitana.

3. The method for producing tagatose according to claim 1 , wherein the epimerization step comprises reacting fructose and enzyme at a temperature ranging from 60° C. to 90° C. and pH 5 to 8.

4. The method for producing tagatose according to claim 3 , wherein the epimerization step is performed in the presence of a metal salt.

5. The method for producing tagatose according to claim 4 , wherein the metal salt comprises at least one of NiSO 4 , NiCl 2 , CoCl 2 , MnCl 2 , and ZnSO 4 .

6. The method for producing tagatose according to claim 1 , wherein a rate of epimerization for converting fructose to tagatose is 5% or higher.

7. The method for producing tagatose according to claim 1 , wherein fructose is produced by hydrolysis of sucrose or isomerization of glucose.

8. The method for producing tagatose according to claim 1 , wherein the purification step is performed by at least one of chromatography, fractional crystallization, and ion purification.

9. The method for producing tagatose according to claim 8 , wherein decoloring, desalting or both decoloring and desalting are performed before or after the purification step.

10. The method for producing tagatose according to claim 1 , further comprising crystallizing the purified epimerized product, after the purification step, wherein the purified epimerized product is concentrated before the crystallization step.

11. The method for producing tagatose according to claim 1 , further comprising crystallizing the purified epimerized product, after the purification step, wherein, after step b), unreacted fructose is recycled to step a), or after the crystallization step, feed from which crystals are separated is recycled to step b), or both steps are performed.

12. The method for producing tagatose according to claim 2 , further comprising crystallizing the purified epimerized product, after the purification step, wherein, after step b), unreacted fructose is recycled to step a), or after the crystallization step, feed from which crystals are separated is recycled to step b), or both steps are performed.

13. The method for producing tagatose according to claim 3 , further comprising crystallizing the purified epimerized product, after the purification step, wherein, after step b), unreacted fructose is recycled to step a), or after the crystallization step, feed from which crystals are separated is recycled to step b), or both steps are performed.

14. The method for producing tagatose according to claim 1 , further comprising crystallizing the purified epimerized product, after the purification step.

Assignees

• Cj Cheiljedang Corp

Inventors

• Yang Sung Jae
• Kim Yang Hee
• Kim Seong Bo
• Park Seung Won
• Park Il Hyang
• Kim Min Hae
• Lee Young Mi

Classifications

• C12P19/02 Primary

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P19/24

  produced by the action of an isomerase, e.g. fructose · CPC title

• Y02P20/582

  Recycling of unreacted starting or intermediate materials · CPC title

• C12Y501/03

  acting on carbohydrates and derivatives (5.1.3) · CPC title

• C12N9/90 Primary

  Isomerases (5.) · CPC title