Electrowetting dispensing devices and related methods

Patent metadata

Field	Value
Publication number	US-10739307-B2
Application number	US-201916258515-A
Country	US
Kind code	B2
Filing date	Jan 25, 2019
Priority date	Aug 26, 2004
Publication date	Aug 11, 2020
Grant date	Aug 11, 2020

Abstract

A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.

First claim

What is claimed is:

1. An electrowetting system, comprising:
a routing plate including a substantially planar array of independently addressable electrodes embedded in a substrate and configured to position liquid at a set of locations, the routing plate including an insulating layer separating the planar array of independently addressable electrodes from a volume;
a nozzle plate disposed over the routing plate to define the volume therebetween, the nozzle plate including a plurality of through holes terminating in a plurality of nozzles, the plurality of through holes aligned with the set of locations, the nozzle plate including a conducting electrode; and
a controller in electrical communication with the planar array of independently addressable electrodes and the conducting electrode, the controller programmed to provide electrical signals to the independently addressable electrodes.

2. The electrowetting system of claim 1 , wherein the plurality of nozzles have a tapered structure disposed on an opposite side of the nozzle plate from the routing plate.

3. The electrowetting system of claim 1 , wherein the routing plate and the nozzle plate are coupled and separated by a seal.

4. The electrowetting system of claim 1 , wherein the routing plate includes a filler channel.

5. The electrowetting system of claim 4 , wherein the filler channel is disposed along an edge of the routing plate.

6. The electrowetting system of claim 4 , wherein the routing plate includes a plurality of side arms in fluid communication with the filler channel.

7. The electrowetting system of claim 6 , wherein the set of locations is disposed along the plurality of side arms.

8. The electrowetting system of claim 7 , wherein the controller is further programmed to cause the addressable electrodes to form discrete droplets along the side arms.

9. The electrowetting system of claim 8 , wherein the controller is to align the discrete droplets with the set of locations.

10. The electrowetting system of claim 4 , further comprising an input port in fluid communication with the filler rail.

11. The electrowetting system of claim 1 , wherein the nozzle plate includes a hydrophobic coating on a side facing the routing plate.

12. The electrowetting system of claim 1 , wherein the insulating layer forms a hydrophobic surface.

13. The electrowetting system of claim 1 , wherein the addressable electrodes are configured to alter a wettability of a surface portion of the insulating layer at a liquid/insulator interface within the volume.

14. The electrowetting system of claim 1 , wherein the controller is further programmed to convert the first insulating layer proximate to a given addressable electrode from hydrophilic to hydrophobic.

15. The electrowetting system of claim 1 , wherein the nozzle plate includes a dielectric material formed over the conductive electrode.

16. The electrowetting system of claim 1 , wherein the addressable electrodes embedded in the substrate comprises a network of pathways comprising the addressable electrodes arranged in an array having rows and columns.

17. The electrowetting system of claim 1 , further comprising a workstation including a sample storage station, a titer plate station, a reagent storage, and a wash station.

18. The electrowetting system of claim 17 , wherein the workstation further includes a thermal cycling station and a sample preparation station.

Assignees

• Applied Biosystems Llc

Inventors

• Vann Charles S
• Banerjee Debjyoti
• Geiser Timothy G
• Nurse James C
• Beard Nigel P

Classifications

• B01L3/0241 Primary

  Drop counters; Drop formers · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title

• B01L3/502792

  for moving individual droplets on a plate, e.g. by locally altering surface tension · CPC title

• B01L3/0268

  using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries · CPC title

• B01J2219/00653

  the compounds being bound to electrodes embedded in or on the solid supports · CPC title