Cell washing using acoustic waves

Patent metadata

Field	Value
Publication number	US-10737012-B2
Application number	US-201615217635-A
Country	US
Kind code	B2
Filing date	Jul 22, 2016
Priority date	Mar 31, 2015
Publication date	Aug 11, 2020
Grant date	Aug 11, 2020

Abstract

Disclosed is a device for separating a cellular component from a multicomponent fluid. The device can include a body, a first acoustic wave generator, and a second acoustic wave propagating component. The body can define a channel having a first surface, an opposing second surface, a first side, and an opposing second side. The channel can extend along a longitudinal axis from a first end to an opposing second end. The first acoustic wave generator can be coupled to the first surface. The second acoustic wave propagating component can be coupled to the second surface. The first acoustic wave generator and second acoustic wave propagating component can be configured to generate a bulk standing acoustic wave in the channel.

First claim

What is claimed is:

1. A device for separating a cellular component from a multicomponent fluid, comprising:
a body defining a channel having a first surface and a second surface opposite the first surface, the channel extending along a longitudinal axis from a first end to a second end, the channel including a mixing region proximate the first end and a collection region proximate the second end, the mixing region configured to facilitate mixing of the multicomponent fluid and a wash material into a mixture;
a first acoustic wave generator coupled to the first surface closer to the second end of the channel than to the first end of the channel, the first acoustic wave generator configured to generate an acoustic wave having a wavelength; and
a second acoustic wave propagating component coupled to the second surface,
wherein the second surface is spaced an integer fractional multiple of the wavelength from the first surface and each integer factional multiple equals a number of pressure nodes within the channel.

2. The device of claim 1 , wherein a central power generating region of the first acoustic wave generator is aligned with the second end of the channel and proximate a bifurcation region of the channel.

3. The device of claim 1 , wherein the integer fractional multiple is 0.5 and the number of pressure nodes is 1.

4. The device of claim 1 , wherein the first acoustic wave generator and the second wave propagating component are located proximate a midpoint of the channel.

5. The device of claim 1 , wherein the body comprises a phantom material forming at least a portion of one or both of the first surface and the second surface, wherein the phantom material has acoustic properties similar to those of the multicomponent fluid and a thickness such that at least one of the pressure nodes is located proximate the phantom material.

6. The device of claim 1 , further comprising a first inlet and a second inlet proximate the first end, the first inlet having a higher elevation than the second inlet.

7. The device of claim 6 , further comprising a first outlet and a second outlet proximate the second end, the second outlet having a higher elevation than the first outlet.

8. The device of claim 7 , wherein the first inlet is configured to receive the wash material and the second inlet is configured to receive a multicomponent mixture.

9. The device of claim 8 , wherein the second outlet is arranged to receive the cellular component and the first outlet is arranged to receive the wash material.

10. The device of claim 1 , wherein the channel has a cross-sectional width and height, wherein an aspect ratio of width:
height is from about 1:11 to about 50:1, and the first acoustic wave generator produces waves having a frequency of from about 100 kHz to about 2000 kHz.

11. The device of claim 1 , wherein, during use, an antinode is formed at approximately the center of the channel and a first pressure node is formed at the first surface and a second pressure node is formed at the second surface.

12. A device for separating a cellular component from a multicomponent fluid, comprising:
a body defining a channel having a first surface and a second surface opposite the first surface, the channel extending along a longitudinal axis from a first end to a second end, the channel defining a mixing region proximate the first end and a bifurcation region proximate the second end, wherein the mixing region is configured to facilitate mixing of the multicomponent fluid and a wash material;
a first acoustic wave generator coupled to the first surface closer to the second end than the first end of the channel, the first acoustic wave generator configured to generate an acoustic wave having a wavelength, the first acoustic wave generator having a central power generating region aligned proximate the bifurcation region; and
a second acoustic wave propagating component coupled to the second surface,
wherein the second surface is spaced from the first surface such that, during use, an antinode is formed at approximately the center of the channel and a first pressure node is formed at the first surface and a second pressure node is formed at the second surface.

13. The device of claim 12 , wherein the body comprises a phantom material forming at least a portion of one or both of the first surface and the second surface, wherein the phantom material has acoustic properties similar to those of the multicomponent fluid and a thickness such that at least one of the pressure nodes is located proximate the phantom material.

14. The device of claim 12 , wherein the first acoustic wave generator or the second wave propagating component is a resonator.

15. The device of claim 12 , further comprising a first inlet and a second inlet proximate the first end, the first inlet having a higher elevation than the second inlet.

16. The device of claim 15 , further comprising a first outlet and a second outlet proximate the second end, the second outlet having a higher elevation than the first outlet.

17. The device of claim 16 , wherein the first inlet is configured to receive the wash material and the second inlet is configured to receive a multicomponent mixture.

18. The device of claim 17 , wherein the second outlet is arranged to receive the cellular component and the first outlet is arranged to receive the wash material.

19. A method of separating a cellular component from cellular component liquid stream, comprising:
introducing the cellular component liquid stream and a wash material into an acoustic wave separation device having a channel extending along a longitudinal axis from a first end to a second end, the channel defining a mixing region proximate the first end, the channel defining a collection region proximate the second end, the collection region including a first outlet and a second outlet;
combining the cellular component liquid stream and the wash material in the mixing region such that the mixing region contains a composition of the cellular component liquid stream and the wash material;
producing a standing acoustic wave using an acoustic wave generator positioned closer to the second end of the channel than to the first end of the channel, the standing acoustic wave having a pressure node located proximate the bifurcation region thereby forcing the cellular component from the cellular component liquid stream; and
collecting the concentrated cellular component in the second outlet.

20. The method of claim 19 , wherein the standing acoustic wave is a surface acoustic wave.

Assignees

• Biomet Biologics Llc
• Biomet Biologics Inc

Inventors

• Leach Michael D
• Hamman Ned M
• Abeskaron David
• Cunningham Grant
• Dorian Randel
• Storrs Richard Wood
• King Scott R

Classifications

• B01D21/283

  Settling tanks provided with vibrators · CPC title

• A61M2202/0429

  Red blood cells; Erythrocytes · CPC title

• B01L2300/0874

  Three dimensional network · CPC title

• C12M47/02

  Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02) · CPC title

• B01L9/527

  for microfluidic devices, e.g. used for lab-on-a-chip · CPC title