Biomolecular patterning of three dimensional tissue scaffolds

Patent metadata

Field	Value
Publication number	US-10736992-B2
Application number	US-201214001327-A
Country	US
Kind code	B2
Filing date	Feb 27, 2012
Priority date	Feb 25, 2011
Publication date	Aug 11, 2020
Grant date	Aug 11, 2020

Abstract

The invention provides methods and compositions for making and using collagen-glycosaminoglycan three-dimensional scaffolds immobilized with biomolecules that are spatially and temporally patterned. The method comprises adding benzophenone to a collagen-glycosaminoglycan three dimensional scaffold in the dark; adding one or more biomolecules to one or more areas of the collagen-glycosaminoglycan three-dimensional scaffold (which can be done optionally in the dark or in the light); and exposing the collagen-2glycosaminoglycan three-dimensional scaffold to light at a wavelength of about 350 to about 365 nm.

First claim

We claim:

1. A method of immobilizing one or more types of biomolecules onto a collagen-glycosaminoglycan three-dimensional scaffold comprising:
(a) adding benzophenone to a collagen-glycosaminoglycan three-dimensional scaffold in the dark;
(b) adding one or more biomolecules to one or more areas of the collagen-glycosaminoglycan three-dimensional scaffold in the dark; and
(c) exposing the collagen-glycosaminoglycan three-dimensional scaffold to light at a wavelength of about 350 to about 365 nm.

2. The method of claim 1 , wherein one or more portions of the collagen-glycosaminoglycan three-dimensional scaffold are exposed to the light while one or more other portions of the scaffold remain in the dark.

3. The method of claim 2 , wherein the one or more types of biomolecules are immobilized onto the scaffold at two or more different depths into the scaffold.

4. The method of claim 1 , wherein two or more types of biomolecules are immobilized to the scaffold, and wherein the two or more types of biomolecules are immobilized to two or more areas of the scaffold, or to two or more depths in the scaffold, or both.

5. The method of claim 1 , wherein a first mask is placed over the collagen-glycosaminoglycan three-dimensional scaffold prior to exposing the collagen-glycosaminoglycan three-dimensional scaffold to the light.

6. The method of claim 5 , further comprising:
(d) placing the collagen-glycosaminoglycan three-dimensional scaffold in the dark and removing the first mask from the collagen-glycosaminoglycan three-dimensional scaffold;
(e) adding one or more biomolecules to one or more areas the collagen-glycosaminoglycan three-dimensional scaffold in the dark;
(f) placing a second mask over the collagen-glycosaminoglycan three-dimensional scaffold in the dark;
(g) exposing the collagen-glycosaminoglycan three-dimensional scaffold to light at a wavelength of about 350 to about 365 nm; and optionally repeating steps (d) to (g) with additional masks and biomolecules.

7. The method of claim 1 , wherein the one or more of the biomolecules are immobilized to the scaffold via a cleavable linker.

8. The method of claim 7 , wherein the cleavable linker is cleavable by an exogenous cue.

9. The method of claim 1 , wherein the one or more biomolecules are peptides, proteins, carbohydrates, cytokines, hormones, nucleic acid molecules, integrins, antibodies, specific binding fragments of antibodies, or a biological sample.

10. The method of claim 7 , wherein the cleavable linker is cleavable by an endogenous cue.

11. The method of claim 1 , further comprising (d) seeding the collagen-glycosaminoglycan three-dimensional scaffold with cells.

Assignees

• Bailey Ryan C
• Harley Brendan A
• Martin Teresa A
• Caliari Steven R
• Board Of Trustees Of The Univ Of Illinois

Inventors

• Bailey Ryan C
• Harley Brendan A
• Martin Teresa A
• Caliari Steven R

Classifications

• A61K35/12

  Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells (vaccines or medicinal preparations containing antigens or antibodies A61K39/00) · CPC title

• A61L27/56

  Porous materials, {e.g. foams or sponges} · CPC title

• C12N5/0657

  Cardiomyocytes; Heart cells · CPC title

• A61L2430/30

  for muscle reconstruction · CPC title

• A61L27/38 Primary

  containing added animal cells (organs or tissue containing native cells A61L27/36) · CPC title