Genetic markers associated with drought tolerance in maize

Patent metadata

Field	Value
Publication number	US-10736289-B2
Application number	US-201816015936-A
Country	US
Kind code	B2
Filing date	Jun 22, 2018
Priority date	Dec 23, 2009
Publication date	Aug 11, 2020
Grant date	Aug 11, 2020

Abstract

The presently disclosed subject matter relates to methods and compositions for identifying, selecting, and/or producing drought tolerant maize plants or germplasm. Maize plants or germplasm that have been identified, selected, and/or produced by any of the methods of the presently disclosed subject matter are also provided.

First claim

What is claimed is:

1. A method of producing a maize plant having improved water optimization, the method comprising the steps of:
a) isolating at least one nucleic acid from a maize plant selected from a population of maize plants;
b) detecting by way of amplifying a marker locus or a portion of a marker locus from the nucleic acid of a) and thereby, producing a amplicon diagnostic for at least one allele located within 10 cM to a water optimization locus that is associated with increased water optimization, wherein the water optimization locus comprises the maize genomic region ZmDhn1, and further wherein ZmDhn1 comprises the following alleles corresponding to SEQ ID NO: 36 comprising a C nucleotide at the position that corresponds to position 500 of SEQ ID NO: 36, a G nucleotide at the position that corresponds to position 568 of SEQ ID NO: 36, and a T nucleotide at the position that corresponds to position 698 of SEQ ID NO: 36 on chromosome 6 in the first plant's genome;
c) selecting a first maize plant from the population of a) based on the presence of the water optimization locus in b);
d) crossing the first maize plant with a second maize plant, wherein the second maize plant does not have in its genome the water optimization locus or allele detected in b); and
e) producing a progeny maize plant having improved water optimization from the cross of d) wherein said progeny plant comprises the water optimization locus as well as the allele detected in b).

2. The method of claim 1 , wherein the second maize plant or progeny maize plant in e) is backcrossed one or more times.

3. The method of claim 1 , wherein the water optimization locus is on Zea mays chromosome 6.

4. The method of claim 1 , wherein the genomic region consist of SEQ ID NO: 36.

5. The method of claim 1 , wherein either the first maize plant or second maize plant is a inbred.

6. The method of claim 1 , wherein the water optimization locus comprises haplotype I.

7. The method of claim 1 , wherein the nucleotide probe or primer used to detect at least one allele within a water optimization locus that is associated with increased water optimization in the first maize plant comprises SEQ ID NO: 95.

8. A maize plant having introgressed into its genome at least one allele detected in the maize parental line within a water optimization locus that is associated with increased water optimization, wherein the water optimization locus comprises the Zea maize genomic region ZmDhn1 and, and further wherein ZmDhn1 comprises the following alleles corresponding to SEQ ID NO: 36 comprising a C nucleotide at the position that corresponds to position 500 of SEQ ID NO: 36, a G nucleotide at the position that corresponds to position 568 of SEQ ID NO: 36, and a T nucleotide at the position that corresponds to position 698 of SEQ ID NO: 36 on chromosome 6 in the first plant's genome.

9. The Zea maize plant of claim 8 , wherein the water optimization locus is located on Zea mays chromosome 6.

10. The maize plant of claim 8 , wherein the maize genomic region consists of SEQ ID NO: 95.

11. The maize plant of claim 8 , wherein the maize plant is an elite maize plant.

12. The maize plant of claim 8 , wherein the water optimization locus comprises haplotype I.

Assignees

• Syngenta Participations Ag

Inventors

• Kishore Venkata Krishna
• Altendorf Paul
• Prest Thomas Joseph
• Zinselmeier Chris
• Wang Daolong
• Briggs William
• Gandhi Sonali
• Foster David
• Chaulk-Grace Christine
• Clarke Joseph Dallas
• Sessions Allen
• Kust Kari Denise
• Reinders Jon Aaron Tucker
• Gutierrez Rojas Libardo Andres
• Li Meijuan
• Warner Todd
• Martin Nicolas
• Miller Robert Lynn
• Arbuckle John
• Skalla Dale Wayne
• Dunn Molly
• Dace Gayle
• Kramer Vance Cary

Classifications

• A01H1/045

  using molecular markers · CPC title

• A01H6/4684

  Zea mays [maize] · CPC title

• A01H1/00 Primary

  Processes for modifying genotypes {; Plants characterised by associated natural traits} (A01H4/00 takes precedence) · CPC title

• A01H5/10 Primary

  Seeds · CPC title

• C12Q2600/172

  Haplotypes · CPC title