Blood analysis systems and methods

Patent metadata

Field	Value
Publication number	US-10732189-B2
Application number	US-201615543579-A
Country	US
Kind code	B2
Filing date	Jan 14, 2016
Priority date	Jan 14, 2015
Publication date	Aug 4, 2020
Grant date	Aug 4, 2020

Abstract

Blood typing systems and methods are provided. In one embodiment, the method may be achieved by applying a sample to a surface of a substrate having one or more binding agents immobilized thereon, wherein the one or more binding agents are capable of binding to one or more substances in the sample; substantially removing unbound material from at least a portion of the substrate having immobilized binding agent; and detecting substances bound to the one or more binding agents immobilized on the substrate; wherein the applying the sample to the surface of the substrate step is concurrent with the removing unbound material from at least a portion of the substrate step. Systems and other methods are also described and illustrated.

First claim

The invention claimed is:

1. A method of determining the presence or absence of a substance in a sample, the method comprising:
applying the sample to a surface of a substrate having a binding agent immobilized thereon, wherein the binding agent is capable of binding to the substance in the sample and the binding agent comprises one or more antibodies to antigens bound at the surface of red blood cells, one or more native or hemolyzed phenotyped red blood cells, one or more recombinant antigens, or one or more antibodies to red blood cell antigens and one or more native or hemolyzed phenotyped red blood cells; and
removing unbound material from at least a portion of the substrate having immobilized binding agent;
wherein binding of the substance to binding agent immobilized on the substrate indicates the substance is present in the sample and the absence of binding of the substance to the binding agent indicates that the substance is absent from the sample; and
wherein the applying the sample to the surface of the substrate step is concurrent with the removing unbound material from at least a portion of the substrate step and is performed with a dispenser configured to simultaneously dispense the sample onto the substrate and to remove unbound material from the substrate.

2. The method of claim 1 , wherein the dispenser that applies the sample to the surface of the substrate and removes unbound substance from at least a portion of the substrate is a hydrodynamic flow confinement dispenser.

3. The method of claim 2 , wherein the hydrodynamic flow confinement dispenser is a microfluidic probe.

4. The method of claim 3 , wherein the hydrodynamic flow confinement dispenser is a microfluidic probe having a plurality of microchannels.

5. The method of claim 3 , wherein the hydrodynamic flow confinement dispenser is an array of microfluidic probes.

6. The method of claim 3 , wherein the hydrodynamic flow confinement dispenser is a microfluidic probe having a microchannel that excludes red blood cells based on size.

7. The method of claim 6 , wherein the microchannel includes a cross section having a diameter of less than 6 micrometers.

8. The method of claim 1 , wherein the surface of the substrate on which binding agent is immobilized is wet.

9. The method of claim 1 , wherein the applying a sample to the surface of the substrate step comprises dispensing one or more samples each in at least one discreet path.

10. The method of claim 9 , wherein the path is a straight line.

11. The method of claim 9 , wherein the path is from between 25 nanometers to 500 micrometers wide.

12. The method of claim 1 , wherein the applying a sample to the surface of the substrate step comprises dispensing one or more samples each in at least one discreet spot.

13. The method of claim 1 , wherein the sample comprises a component selected from the group consisting of whole blood, red blood cells, plasma, serum, and saliva.

14. The method of claim 1 , wherein the binding agent is immobilized in a discreet line or is immobilized in a discreet spot.

15. A method of determining the presence or absence of a substance in a sample, the method comprising:
applying the sample to a surface of a substrate having a binding agent immobilized thereon, wherein the binding agent is capable of binding to the substance in the sample;
removing unbound material from at least a portion of the substrate having immobilized binding agent; and
responsive to detecting the substance bound to the binding agent immobilized on the substrate, identifying the substance present in the sample;
and responsive to not detecting the substance bound to the binding agent immobilized on the substrate, determining that the substance is absent in the sample;
wherein the applying of the sample to the surface of the substrate step is concurrent with the removing of unbound material from at least a portion of the substrate step; and
wherein the applying of the sample to the surface of the substrate and the removing of unbound material from at least a portion of the substrate steps are performed with a hydrodynamic flow confinement dispenser comprising a microfluidic probe having a microchannel that excludes red blood cells based on size.

16. The method of claim 15 , wherein the dispenser is a microfluidic probe having a plurality of microchannels.

17. The method of claim 15 , wherein the dispenser is an array of microfluidic probes.

18. The method of claim 15 , wherein the microchannel includes a cross section having a diameter of less than 6 micrometers.

19. The method of claim 15 , wherein the surface of the substrate on which binding agent is immobilized is wet.

20. The method of claim 15 , wherein the applying a sample to the surface of the substrate step comprises dispensing one or more samples each in at least one discreet path.

21. The method of claim 20 , wherein the path is a straight line.

22. The method of claim 20 , wherein the path is from between 25 nanometers to 500 micrometers wide.

23. The method of claim 15 , wherein the applying a sample to the surface of the substrate step comprises dispensing one or more samples each in at least one discreet spot.

24. The method of claim 15 , wherein the sample comprises a component selected from the group consisting of whole blood, red blood cells, plasma, serum, and saliva.

25. The method of claim 15 , wherein the binding agent comprises one or more antibodies to antigens bound at the surface of red blood cells, one or more native or hemolyzed phenotyped red blood cells, one or more recombinant antigens, or one or more antibodies to red blood cell antigens and one or more native or hemolyzed phenotyped red blood cells.

26. The method of claim 15 , wherein the binding agent is immobilized in a discreet line or is immobilized in a discreet spot.

Assignees

• Bio Rad Laboratories
• Bio Rad Innovations
• Buffiere Frederic
• Villard Saussine Sylvie
• Rivalin Eliane
• Guillon Laurent
• Guegan Johann
• Bio Rad Europe Gmbh

Inventors

• Buffiere Frederic
• Villard-Saussine Sylvie
• Rivalin Eliane
• Guillon Laurent
• Guegan Johann

Classifications

• G01N33/56966

  Animal cells · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• B01L3/502776 Primary

  specially adapted for focusing or laminating flows · CPC title

• G01N33/54306

  Solid-phase reaction mechanisms · CPC title

• B01L2300/0829

  Multi-well plates; Microtitration plates · CPC title