DIG-305 insecticidal Cry toxins

Patent metadata

Field	Value
Publication number	US-10731176-B2
Application number	US-201515541290-A
Country	US
Kind code	B2
Filing date	Dec 16, 2015
Priority date	Dec 30, 2014
Publication date	Aug 4, 2020
Grant date	Aug 4, 2020

Abstract

DIG-305 insecticidal protein toxins, polynucleotides encoding such toxins, and transgenic plants that produce such toxins are disclosed. Also disclosed are methods for using such toxins to control insect pests in plants, especial crop plants. Methods for preparing transgenic plants expressing the protein toxins and methods for detecting the claimed toxins and polynucleotides in transgenic plants are disclosed.

First claim

We claim:

1. An isolated, treated, or formulated dig-305 insecticidal toxin polypeptide comprising a core toxin segment that includes an amino acid sequence selected from the group consisting of:
(a) residues 2 to 685 of SEQ ID NO:2; and
(b) a sequence having at least 99% sequence identity to the amino acid sequence of residues 2 to 685 of SEQ ID NO:2;
wherein the core toxin segment is linked to a C-terminal protoxin portion of a Cry toxin other than DIG-305.

2. The isolated, treated, or formulated polypeptide of claim 1 , wherein the polypeptide is a chimeric protein and the C-terminal protoxin portion comprises the C-terminal protoxin portion of cry1Ab or a cry1Ac/cry1Ab chimeric toxin.

3. The isolated, treated, or formulated polypeptide of claim 2 , wherein the C-terminal protoxin portion comprises the C-terminal protoxin portion of Cry1Ab.

4. The isolated, treated, or formulated polypeptide of claim 2 , wherein the C-terminal protoxin portion comprises the C-terminal protoxin portion of cry1Ac/cry1Ab chimeric toxin.

5. A method for controlling a pest population, said method comprising contacting said population with a pesticidally effective amount of the polypeptide of claim 1 .

6. The polypeptide of claim 1 having activity against a coleopteran pest.

7. The polypeptide of claim 1 having activity against corn rootworm.

8. A composition comprising the polypeptide of claim 1 .

9. The composition of claim 8 , wherein the composition is a sprayable protein composition, encapsulated protein composition, or bait matrix that comprises a formulated DIG-305 insecticidal toxin.

10. A nucleic acid construct, wherein the construct comprises a heterologous nucleic acid sequence that is recombinantly linked to a sequence encoding a DIG-305 insecticidal toxin comprising a core toxin segment that includes an amino acid sequence selected from the group consisting of:
(a) residues 2 to 685 of SEQ ID NO:2; and
(b) a sequence having at least 99% sequence identity to the amino acid sequence of residues 2 to 685 of SEQ ID NO:2.

11. The nucleic acid construct of claim 10 , wherein the heterologous nucleic acid sequence is a promoter sequence capable of driving expression in a plant.

12. The nucleic acid construct of claim 10 , wherein the sequence encoding the polypeptide is codon-optimized for expression in a plant.

13. The nucleic acid construct of claim 11 , wherein the promoter is capable of driving expression in corn and the sequence encoding the polypeptide is codon optimized for expression in corn.

14. The nucleic acid construct of claim 10 , wherein the sequence encoding the polypeptide comprises SEQ ID NO:1 or SEQ ID NO:3.

15. The nucleic acid construct of claim 13 , wherein the construct is a vector and the vector comprises SEQ ID NO:3.

16. The nucleic acid construct of claim 11 , wherein the promoter is capable of driving expression in potato and the sequence encoding the polypeptide is codon optimized for expression in potato.

17. A transgenic plant comprising the nucleic acid construct of claim 10 stably incorporated into its genome.

18. A method for protecting a plant from a pest, said method comprising introducing into said plant the construct of claim 10 .

Assignees

• Dow Agrosciences Llc

Inventors

• Hey Timothy D
• Frey Meghan L F
• Xu Xiaoping
• Etter Audrey Jane
• Caldwell Elizabeth
• Letherer Ted
• Elango Navin
• Narva Kenneth

Classifications

• C12N15/8286 Primary

  for insect resistance · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• A01N37/46

  N-acyl derivatives · CPC title