Bag assembly for cultivation of cells

Patent metadata

Field	Value
Publication number	US-10731118-B2
Application number	US-201615363562-A
Country	US
Kind code	B2
Filing date	Nov 29, 2016
Priority date	May 28, 2014
Publication date	Aug 4, 2020
Grant date	Aug 4, 2020

Abstract

Provided is a bioreactor assembly that includes a plurality of cultivation bags at least one flexible temperature control bag having a thermostating fluid, and a tray adapted to rock back and forth around at least one axis. The flexible temperature control bag is mounted on the tray and the cultivation bags are located on the flexible temperature control bag.

First claim

The invention claimed is:

1. A bioreactor assembly comprising:
a) a plurality of independent cultivation bags, wherein the plurality of independent cultivation bags are fluidically connected in series through a respective valve between each adjacent pair of independent cultivation bags of the plurality of independent bags;
b) one flexible temperature control bag having a top surface directly contacting a bottom surface of each independent cultivation bag of the plurality of independent cultivation bags, and configured to contain a thermostating fluid to facilitate heat transfer between the one flexible temperature control bag and a cell culture in the plurality of independent cultivation bags, and;
c) a tray adapted to rock back and forth around at least one axis,
wherein said flexible temperature control bag is mounted on said tray and said cultivation bags are seated on said top surface of the flexible temperature control bag, and
wherein the cultivation bags are secured relative to the flexible temperature control bag through attachment to at least one of the flexible temperature control bag or the tray.

2. The bioreactor assembly of claim 1 , wherein said temperature control bag comprises an inlet and an outlet for the thermostating fluid.

3. The bioreactor assembly of claim 1 , wherein each cultivation bag comprises a gas inlet and a gas outlet.

4. The bioreactor assembly of claim 1 , wherein each cultivation bag comprises at least two hollow tabs adapted for insertion of a bag connector rod and a tray connector rod through said tabs.

5. The bioreactor assembly of claim 1 , wherein said flexible temperature control bag comprises a plurality of loops adapted for insertion of a bag connector rod for securing the cultivation bags.

6. The bioreactor assembly of claim 1 , wherein said cultivation bags are secured to said tray by tensioners.

7. The bioreactor assembly of claim 6 , wherein said tensioners comprise straps.

8. The bioreactor assembly of claim 6 , wherein said tensioners comprise separable fabric hook and loop fastener straps.

9. The bioreactor assembly of claim 1 , wherein the independent cultivation bag has an inflated volume of 150-1000 ml.

10. The bioreactor assembly of claim 9 , wherein the independent cultivation bag has an elongated hexagonal or octagonal shape.

11. The bioreactor assembly of claim 9 , wherein the independent cultivation bag is a three-dimensional gusseted bag.

12. The bioreactor assembly of claim 11 , wherein said three-dimensional gusseted bag has an inflated height of 5-10 cm.

13. A flexible cell cultivation bag having an inflated volume of 150-1000 ml and an inflated height of 5-10 cm seated on top of a secondary cultivating bag being used as a flexible temperature control bag having a top surface in direct contact with a bottom surface of the flexible cell cultivation bag, configured to contain a thermostating fluid to facilitate heat transfer between the thermostating fluid and a cell culture in the flexible cultivation bag,
wherein the cultivation bag is secured relative to the at least one flexible temperature control bag through attachment to at least one of the temperature control bag and a tray; and
wherein the cultivation bag is fluidically connected to at least a third cultivation bag through a valve.

14. The flexible cell cultivation bag of claim 13 , equipped with at least two ports located in a top film of the cell cultivation bag.

15. The flexible cell cultivation bag of claim 13 , which is a three-dimensional gusseted bag.

16. The flexible cell cultivation bag of claim 14 , comprising at least one fitting with a plurality of ports in said fitting.

17. The flexible cell cultivation bag of claim 14 , comprising at least one branched port or branched line attached to a port.

18. The flexible cell cultivation bag of claim 17 , wherein at least one port branch is connected to a swabable needleless valve.

19. The flexible cell cultivation bag of claim 13 , comprising one or more fastener elements attached to a top film, or to a top portion of a side film, of said bag, wherein said fastener element or elements is/are arranged to engage with one or more retainer elements on a support device when the bag is inflated.

20. The flexible cell cultivation bag of claim 19 , wherein said fastener element or elements and retainer element or elements are arranged to automatically engage with each other upon inflation of the bag to a predetermined level.

21. An assembly of a flexible bag according to claim 19 , and a support device with one or more retainer elements arranged to engage with said fastener element or elements when the bag is inflated.

22. An assembly of a flexible bag according to claim 13 , comprising the secondary cultivation bag used as the flexible temperature control bag disposed on a tray adapted to rock back and forth around at least one axis.

23. The bioreactor assembly of claim 1 , further comprising a heat control surface disposed between the tray and the flexible temperature control bag, for facilitating heat temperature control.

24. The bioreactor assembly of claim 1 , wherein the plurality of independent cultivation bags comprises a first cultivation bag and a second cultivation bag, wherein the volume of the second cultivation bag is at least 120% of the volume of the first cultivation bag.

25. The bioreactor assembly of claim 24 , wherein the volume of the second cultivation bag is at least 150% of the volume of the first cultivation bag.

Assignees

• Ge Healthcare Bio Sciences Ab
• Ge Healthcare Bio Sciences Corp

Inventors

• Stankowski Ralph
• Lundstrom Asa
• Castan Andreas
• Miller Michael
• Klingberg Yvette

Classifications

• C12M41/22

  in contact with the bioreactor walls · CPC title

• C12M23/48

  Holding appliances; Racks; Supports (holding devices for laboratory apparatus B01L9/00) · CPC title

• C12M27/16

  Vibrating; Shaking; Tilting · CPC title

• C12M23/14 Primary

  Bags · CPC title

• C12M23/34

  Internal compartments or partitions · CPC title