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Biofabrication techniques for the implementation of intrinsic tissue geometries to an in vitro collagen hydrogel
US10730928B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10730928-B2 |
| Application number | US-201515514091-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 28, 2015 |
| Priority date | Sep 26, 2014 |
| Publication date | Aug 4, 2020 |
| Grant date | Aug 4, 2020 |
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Abstract
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Methods for reaction electrospinning are provided to form collagen fibers. The method can include: acidifying a collagen in an acidic solvent to form an acidic collagen solution; electrospinning the acidic collagen solution within an alkaline atmosphere (e.g., including ammonia vapor) to form collagen fibers; and collecting the collagen fibers within a salt bath (e.g., including ammonium sulfate). The acidic solvent can include water and an alcohol, and can have a pH of about 2 to about 4 (e.g., including a strong acid, such as HCl). An albumin rubber is also provided, which can include albumin crosslinked with glutaraldehyde.
First claim
Opening claim text (preview).
What is claimed: 1. A method of forming an engineered bio-scaffold comprising: filling a mold with an albumin rubber such that the albumin rubber conforms to the shape of the mold; removing the albumin rubber from the mold to form a rubber blank; coating the rubber blank with collagen such that the collagen takes the shape of the rubber blank; and selectively dissolving the rubber blank from the inside of the collagen using an enzyme to form the bio-scaffold, wherein the enzyme selectively dissolves the material of the rubber blank while leaving the collagen and forming flow channels inside the bio-scaffold. 2. The method as in claim 1 , wherein the enzyme comprises trypsin. 3. The method as in claim 1 , wherein the enzyme is provided in a solution having a pH of about 7 to about 9.
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the fibre formed by coagulation, i.e. wet electro-spinning · CPC title
of cellulose, cellulose derivatives, or proteins · CPC title
derived from horn, hoofs, hair, skin or leather · CPC title
Albumins · CPC title
derived from leather or skin {, e.g. gelatin} · CPC title
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- What does patent US10730928B2 cover?
- Methods for reaction electrospinning are provided to form collagen fibers. The method can include: acidifying a collagen in an acidic solvent to form an acidic collagen solution; electrospinning the acidic collagen solution within an alkaline atmosphere (e.g., including ammonia vapor) to form collagen fibers; and collecting the collagen fibers within a salt bath (e.g., including ammonium sulfat…
- Who is the assignee on this patent?
- Univ South Carolina, Musc Found For Res Dev
- What technology area does this patent fall under?
- Primary CPC classification C07K14/78. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 04 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).