Compositions and methods comprising endophytic bacterium for application to grasses to increase plant growth, suppress soil borne fungal diseases, and reduce vigor of weedy competitors

Patent metadata

Field	Value
Publication number	US-10721936-B2
Application number	US-201715657100-A
Country	US
Kind code	B2
Filing date	Jul 21, 2017
Priority date	Jul 21, 2016
Publication date	Jul 28, 2020
Grant date	Jul 28, 2020

Abstract

Endophytic bacteria, compositions comprising the same, and methods of use thereof are disclosed which increase the root and shoot growth of grass hosts, suppress growth of soil borne fungal pathogens of host plants, increase resistance of the grass to diseases and reduce competitiveness of distantly related competitor weeds of the crop.

First claim

What is claimed is:

1. A synthetic combination comprising at least one purified bacterial population in association with a seed of a turf and forage grass, wherein the purified bacterial population comprises a seed bacterial endophyte, SY1 deposited as NRRL-B67481, that is heterologously disposed to the turf or forage grass seed, wherein the seed bacterial endophyte is present in the synthetic combination at a concentration of at least 10 3 CFU/seed on the surface of a seed, and in an amount effective to increase one or more of root growth promotion, shoot growth promotion, resistance to salt stress, competition with undesirable plant species and increase resistance to fungus in a plant produced from the seed, as compared to a reference plant grown under the same conditions.

2. The synthetic combination of claim 1 , wherein said bacteria are lyophilized.

3. The synthetic combination of claim 1 , wherein said bacteria are encapsulated in an alginate bead formulation.

4. The synthetic combination of claim 1 , wherein said bacteria are present in a seed ball.

5. The synthetic combination of claim 1 , wherein said combination includes at least one of Sandy LB4 deposited as NRRL B-67308, and Microbacterium oxydans B2 deposited as NRRL-B67470, wherein said seed is a rice seed or a grass seed.

6. The synthetic combination of claim 1 , wherein said combination further comprises at least one of Sandy LB6 deposited as NRRL B-67484 and West 9 deposited as NRRL B67307 and wherein said seed is a grass seed from a grass selected from Agrostis spp., Poa spp., Festuca spp., Lolium spp., Cynodon spp., Zoysia spp., Koleria spp., and Danthonia spp.

7. The synthetic combination of claim 5 , further comprising WY14 deposited as NRRL-B-67483 wherein said seed is a rice seed or a grass seed.

8. A method of reducing undesirable weed growth in soils comprising turf and forage grasses, comprising drenching or treating the soil with one or more biologically pure Pseudomonas spp. endophyte strains wherein said strains reduce weed growth relative to weed growth observed in untreated soil, wherein said one or more Pseudomonas spp. is SY1 deposited as NRRL B-67481.

9. The method of claim 8 , further comprising one or more biologically pure endophyte strains Sandy LB4 deposited as NRRL B-67308 and/or Microbacterium oxydans B2 deposited as NRRL-B67470, and/or WY6 comprising SEQ ID NO: 7, and/or WY14 deposited as NRRL-B-67483 wherein said strains are obtained from Phragmites and said endophytes are lyophilized.

10. The synthetic combination of claim 1 , comprising one or more bacteria selected from the group consisting of
i) protease negative strain West 9 deposited as NRRL B67307;
ii) phosphatase positive strain Sandy LB6 deposited as NRRL B-67484;
iii) phosphatase positive strain WY14 deposited as NRRL-B-67483; and
iv) strain SY5 deposited as NRRL B67482.

11. The synthetic combination of claim 10 , wherein said at least one biologically pure endophyte strain is obtained from Phragmites and said bacteria are lyophilized.

12. The synthetic combination of claim 1 further comprising one or more of Sandy LB6 comprising SEQ ID NO: 2 deposited as NRRL B-67484, Sandy Y8 comprising SEQ ID NO: 3 and deposited under NRRL B-67896, RoY12 comprising SEQ ID NO: 6 and deposited under NRRL B-67898, RoLB13w comprising SEQ ID NO: 5 and deposited under NRRL B-67894, West 9 comprising SEQ ID NO: 10 and deposited as NRRL B-67307, RiY3 comprising SEQ ID NO: 4 and deposited under NRRL B-67893, SandyY7w comprising SEQ ID NO: 13 and deposited under NRRL B-67895, RiLB4 comprising SEQ ID NO:14 and deposited under NRRL B-67897, and SY5 comprising SEQ ID NO: 16 and deposited as NRRL B-67482.

13. A method of increasing root and shoot growth of a grass plant host, comprising inoculating the host grass or seed with the synthetic combination of claim 1 , said synthetic combination being effective to increase one or more of root growth promotion, shoot growth promotion, resistance to salt stress, competition with undesirable plant species and resistance to fungus in a plant produced from the seed, as compared to a reference plant grown under the same conditions.

14. The method of claim 13 , wherein the inoculated host grass exhibits suppressed growth of soil borne fungal pathogens of said host grass relative to host grasses which have not been inoculated with said synthetic combination.

15. The method of claim 13 , wherein the host grass is a turf and forage grass selected from Agrostis spp., Poa spp., Festuca spp., Lolium spp., Cynodon spp., Zoysia spp., Koleria spp., Paspalum spp., Panicum spp., Stenotaphrum spp., Eremochloa spp. and Danthonia spp., Triticum aestivum, Oryza sativa, Avena sativa, Hordeum vulgare, Panicum miliaceum, Eleusine coracana, Sorghum bicolor, Pennisetum glaucum, Setaria italica, Zea maydis, Agrostis spp., Poa spp., Festuca spp., Lolium spp., Cynodon spp., Zoysia spp., Koleria spp., and Danthonia spp.

16. The method of claim 13 , wherein the host grass is a food grass crop selected from Oryza sativa, Triticum aestivum , and Zea maydis.

17. The method of claim 13 , wherein the host grass is a Pooideae grass.

18. The method of claim 13 , wherein said synthetic combination is present in a synthetic seed ball.

19. The method of claim 13 , wherein said plant host is sprayed with a liquid formulation comprising said synthetic combination.

20. The method of claim 13 , wherein said synthetic combination is present in a controlled release fertilizer formulation.

21. The method of claim 13 , wherein said synthetic combination comprises one or more of Sandy LB4 deposited as NRRL B-67308 and/or Microbacterium oxydans B2 deposited as NRRL-B67470, and/or WY6 comprising SEQ ID NO: 7, and/or WY14 deposited as NRRL-B-67483.

22. The method of claim 13 , wherein said synthetic combination comprises one or more of Sandy LB6 comprising SEQ ID NO: 2 deposited as NRRL B-67484, Sandy Y8 comprising SEQ ID NO: 3 and deposited under NRRL B-67896, RoY12 comprising SEQ ID NO: 6 and deposited under NRRL B-67898, RoLB13w comprising SEQ ID NO: 5 and deposited under NRRL B-67894, West 9 comprising SEQ ID NO: 10 and deposited as NRRL B-67307, RiY3 comprising SEQ ID NO: 4 and deposited under NRRL B-67893, SandyY7w comprising SEQ ID NO: 13 and deposited under NRRL B-67895, RiLB4 comprising SEQ ID NO:14 and deposited under NRRL B-67897, and SY5 comprising SEQ ID NO: 16 and deposited as NRRL B-67482.

Assignees

• Univ Rutgers
• United States Geological Survey
• Rutgers The State Univ Of Nj

Inventors

• White Jr James F
• Kowalski Kurt P
• Kingsley Kathryn

Classifications

• C12R2001/38

  Pseudomonas · CPC title

• C12R2001/39

  Pseudomonas fluorescens · CPC title

• C12R2001/01

  Bacteria or Actinomycetales {; using bacteria or Actinomycetales} · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• C12N1/205 Primary

  Bacterial isolates · CPC title