Flow cell assembly securement system and method

Patent metadata

Field	Value
Publication number	US-10718786-B2
Application number	US-201615359848-A
Country	US
Kind code	B2
Filing date	Nov 23, 2016
Priority date	Nov 23, 2016
Publication date	Jul 21, 2020
Grant date	Jul 21, 2020

Abstract

A flow cell and cartridge assembly may be loaded into a processing system, such as for genetic sequencing. The system locates the assembly and is then actuated to move the assembly to a desired reference position in both X- and Y-directions. Further actuation causes clamps to contact the flow cell, the cartridge, or both to exert a hold-down force during processing. Further hold-down forces may be provided by a vacuum chuck. Fluid connections are also made by manifolds that contact the flow cell. The hold-down forces counteract the forces needed for sealing the manifolds to the flow cell.

First claim

The invention claimed is:

1. A system comprising:
a support configured to receive a flow cell assembly, the support comprising an X-direction locator element;
a common-side securement assembly having a first arm;
a post-side securement assembly having a second arm, the first and second arms actuatable to draw the flow cell assembly into engagement towards the support in a Z-direction to a plane of the flow cell assembly, the common-side securement assembly positioned on a first side of the support and the post-side securement assembly positioned on a second side of the support, the second side opposite the first side;
a first driving element actuatable to urge the flow cell assembly into a first reference position in an X-direction perpendicular to the Z-direction against the X-direction locator element;
a second driving element actuatable to urge the flow cell assembly into a second reference position in a Y-direction perpendicular to the Z-direction and to the X-direction against a Y-direction locator element; and
an actuating system operatively connected to the arms, the first driving element and the second driving element and configured to, in a single securement operation, actuate the arms to draw the flow cell assembly towards the support in the Z-direction, actuate the first driving element to urge the flow cell assembly into the first reference position against the X-direction locator element in the X-direction, and actuate the second driving element to urge the flow cell assembly into the second reference position against the Y-direction locator element in the Y-direction.

2. The system of claim 1 , further comprising fluid connections configured to form a sealed fluid flow path with the flow cell assembly when the flow cell assembly is positioned in the first and second reference positions.

3. The system of claim 2 , wherein the fluid connections comprise manifolds that are moveable by the actuating system to complete sealed fluid connections with an inlet side of the flow cell assembly and an outlet side of the flow cell assembly.

4. The system of claim 3 , wherein the manifolds comprise a first manifold configured to fluidically couple with a first elastomeric element of the flow cell assembly for sealing more than one flow path.

5. The system of claim 2 , wherein the fluid connections comprise manifolds configured to be positioned relative to inlet and outlet sides of the flow cell assembly.

6. The system of claim 5 , comprising springs; wherein the manifolds are spring-biased upwardly via the springs against a force exerted by the arms.

7. The system of claim 1 , wherein the first arm is configured to contact an upper side of the flow cell assembly on an inlet side of the flow cell assembly, and the second arm is configured to contact the upper side of the flow cell assembly on an outlet side of the flow cell assembly.

8. The system of claim 1 , wherein the actuating system includes a pair of electric motors for moving the flow cell assembly towards the first reference position, and a third motor for moving the flow cell towards the second reference position.

9. The system of claim 1 , wherein the arms and the first and second driving elements are each configured to contact at least one of a frame structure of the flow cell assembly or a flow cell of the flow cell assembly when actuated by the actuating system when the flow cell assembly is received by the support.

10. The system of claim 1 , wherein the first driving element comprises a helical surface configured to urge the flow cell assembly towards the first reference position.

11. The system of claim 1 , comprising biasing springs, wherein the arms are biased in a clamped position by the biasing springs.

Assignees

• Ilumina Inc
• Illumina Inc

Inventors

• De Ruyter Anthony John
• Kehl Patrick
• Mcgee Thomas

Classifications

• G01N35/00029

  provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence) · CPC title

• G01N35/1095

  for supplying the samples to flow-through analysers (for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00) · CPC title

• B01J2219/00686

  Automatic · CPC title

• B01J2219/00418

  using pressure · CPC title

• B01J2219/00702

  Processes involving means for analysing and characterising the products · CPC title