Method for making an asymmetrically-tagged sequencing library

Patent metadata

Field	Value
Publication number	US-10711269-B2
Application number	US-201715409124-A
Country	US
Kind code	B2
Filing date	Jan 18, 2017
Priority date	Jan 18, 2017
Publication date	Jul 14, 2020
Grant date	Jul 14, 2020

Abstract

A method for making an asymmetrically-tagged sequencing library is provided. In some embodiments, the method may comprise: obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, hybridizing a tailed first primer to the 3′ sequence tag of the library and extending the same to produce primer extension products, and amplifying the primer extension products using a pair of tailed primers to produce asymmetrically-tagged library.

First claim

The invention claimed is:

1. A method for making an asymmetrically-tagged sequencing library, comprising:
(a) obtaining a symmetrically-tagged library of cDNA or genomic DNA fragments, wherein at least some of the members of the library comprise a top strand comprising a 5′ sequence tag and a 3′ sequence tag, wherein the 5′ and 3′ sequence tags comprise complementary sequences;
(b) hybridizing a first primer to a region of the 3′ sequence tag of the library, wherein the first primer comprises a 3′ region which is complementary to said region of the 3′ sequence tag of the library, and a 5′ non-complementary tail;
(c) extending the first primer to produce primer extension products that comprise, from 5′ to 3′, the sequence of the first primer, a sequence of a fragment, and the complement of a 5′ sequence tag of (a); and
(d) amplifying the primer extension products of (c) using:
i. a forward primer comprising sequence A4; and
ii. a reverse primer of formula A5-A2, wherein sequence A5 is a 5′ tail and sequence A2 is contained in the 5′ sequence tag of (a);

to produce an asymmetrically-tagged library in which at least some of the members comprise a top strand comprising i. a first end comprising sequence A5, ii. the sequence of a fragment, and iii. a second end comprising the complement of sequence A4.

2. The method of claim 1 , wherein the 5′ and 3′ sequence tags of (a) each comprise a double-stranded molecular barcode that varies in sequence in the library.

3. The method of claim 2 , wherein the molecular barcodes are random sequences.

4. The method of claim 1 , wherein the 5′ tail of the first primer of (b) comprises a sample index that varies in sequence.

5. The method of claim 1 , further comprising analyzing the products of step (d).

6. The method of claim 5 , wherein the analyzing comprises sequencing the products of step (d).

7. The method of claim 6 , further determining the sequence of two or more molecular barcodes on the same molecule.

8. The method of claim 7 , further comprising using said molecular barcode sequences to associate sequencing reads from the top strand of a duplex with sequencing reads from the complementary strand.

9. The method of claim 6 , wherein sequence information is obtained from both the top strand and the bottom strand of a duplex.

10. The method of claim 1 , wherein the method comprises removing, inactivating, or destroying any unextended first primer between steps (c) and (d).

11. The method of claim 1 , wherein the library of (a) is made by tagmentation.

12. The method of claim 11 , wherein the tagmentation is done using a Tn5 or Vibhar transposase.

13. The method of claim 1 , wherein the library of (a) is made by ligating adaptors to a population of cDNA or genomic fragments.

14. The method of claim 13 , wherein the cDNA or genomic fragments are made by fragmenting an initial cDNA or genomic sample by physical, chemical or enzymatic means.

15. The method of claim 1 , wherein the library of step (a) is made by (i) making an initially tagged library comprising fragments of cDNA or genomic DNA that comprise sequence tags on both ends, wherein the sequence tags comprise a single-stranded region, and (ii) extending at least some of the 3′ ends of the initially tagged library to make the single-stranded region double-stranded.

16. The method of claim 15 , wherein the single strand region contains a single stranded molecular barcode, and wherein the extending converts the single stranded molecular barcode into a double stranded molecular barcode.

17. The method of claim 16 , wherein the extending is done using a strand-displacing polymerase.

18. The method of claim 16 , wherein the extending is done using a non-strand-displacing polymerase.

19. The method of claim 1 , wherein additional sequences A2 and sequences complementary to A3 are added during hybridization step (b).

20. The method of claim 19 , wherein one or more of the 3′ ends of said additional sequences cannot be extended by a polymerase.

Assignees

• Agilent Technologies Inc

Inventors

• Peter Brian Jon
• Taussig David
• Arezi Bahram
• Ach Robert A
• Sampas Nicholas M

Classifications

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C12N15/1065 Primary

  Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags · CPC title

• C12Q2537/143

  Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis · CPC title

• C12Q2525/191

  incorporating an adaptor · CPC title

• C12Q2533/101

  Primer extension · CPC title