Adipose tissue centrifuge and method of use

Patent metadata

Field	Value
Publication number	US-10711239-B2
Application number	US-201815949714-A
Country	US
Kind code	B2
Filing date	Apr 10, 2018
Priority date	Jan 31, 2014
Publication date	Jul 14, 2020
Grant date	Jul 14, 2020

Abstract

A centrifuge device is provided for the sizing and separation of constituents of a biologic mixture, e.g., adipose tissue. The device provides for the mechanical breaking down of the fibrous structure in the tissue by centrifugation causing the tissue to pass through a mesh element, or a sizing helix, or an extrusion element, whereupon the material is reduced to a slurry. This processed material may then be separated by centrifugation into its constituents, in order to harvest the fraction containing the multipotent cells. These multipotent cells may be utilized for various medical procedures to stimulate healing and tissue regeneration.

First claim

What is claimed is:

1. A method for processing a biologic mixture and selectively concentrating constituents of the biologic mixture, the constituents having differing specific gravities and being stratifiable in a centrifugal field, said method comprising:
a. introducing a biologic mixture comprising adipose tissue into a chamber of a centrifuge, said chamber comprising a sidewall with a tapered inner surface, a base, an annular screen having an inner surface, and a first port; and
b. rotating said chamber about an axis to pass at least a substantial portion of said adipose tissue through said annular screen, thereby morselizing the substantial portion of said adipose tissue; and causing the biologic mixture to stratify in said chamber into at least two concentric stratified constituent layers.

2. The method of claim 1 , wherein the biologic mixture comprises adipose tissue and water based liquids.

3. The method of claim 1 , wherein one of the at least two concentric stratified constituent layers comprise multipotent cells.

4. The method of claim 3 , further comprising the step of harvesting said multipotent cells from said centrifuge.

5. The method of claim 1 , wherein the annular screen has an inner surface located at a first radial distance from the axis, the annular screen projecting away from the base.

6. The method of claim 5 , wherein the centrifuge further comprises a roller arranged to roll around the inner surface of the annular screen and urge at least a portion of the biologic mixture through the annular screen and away from the axis and towards the sidewall.

7. The method of claim 5 , wherein the centrifuge further comprises a roller arranged to roll against and around the inner surface of the annular screen and urge at least a portion of the biologic mixture through the annular screen and away from the axis and towards the sidewall.

8. The method of claim 1 , wherein the centrifuge further comprises a first port having an inlet at a second radial distance from said axis, wherein the first port is selectively openable to allow the discharge of at least a portion of a constituent from the chamber.

9. The method of claim 6 , wherein the chamber further comprises at least one roller axle extending parallel to said axis, said roller being arranged to spin about said at least one roller axle to roll around said inner surface of said annular screen.

10. The method of claim 9 , wherein said at least one roller axle is held stationery relative to said chamber.

11. The method of claim 7 , wherein the chamber further comprises at least one roller axle extending parallel to said axis, said roller being arranged to spin about said at least one roller axle to roll around said inner surface of said annular screen and wherein said at least one roller axle is held stationery relative to said chamber.

12. The method of claim 1 , wherein said annular screen is a perforated material selected from the group consisting of wire mesh screen, polymer mesh screen, perforated metal tubing and perforated polymer tubing.

13. The method of claim 1 , wherein the chamber further comprises a trap, said trap being located in said chamber adjacent said inner surface of said sidewall, and said chamber, and wherein the centrifuge further comprises at least one port and a valve for selectively ejecting at least a portion of at least one of said constituent layers from the trap.

14. The method of claim 1 , wherein the annular screen comprises plural openings in the range of approximately 0.04 inches to approximately 0.002 inches.

15. The method of claim 1 , wherein the annular screen rotates at the same speed as the chamber.

16. The method of claim 5 , wherein the chamber further comprises a secondary screen, the secondary screen being located at a fifth radial distance from the axis, wherein the fifth radial distance is greater than the first radial distance, wherein the secondary annular screen has a smaller opening size than the annular screen, and wherein the secondary annular screen is configured to capture fibrous material while allowing non-fibrous material and liquid to pass through.

17. The method of claim 6 , wherein the chamber further comprises a secondary screen, the secondary screen being located at a fifth radial distance from the axis, wherein the fifth radial distance is greater than the first radial distance, wherein the secondary annular screen has a smaller opening size than the annular screen, and wherein the secondary annular screen is configured to capture fibrous material while allowing non-fibrous material and liquid to pass through.

18. The method of claim 11 , wherein the chamber further comprises a secondary screen, the secondary screen being located at a fifth radial distance from the axis, wherein the fifth radial distance is greater than the first radial distance, wherein the secondary annular screen has a smaller opening size than the annular screen, and wherein the secondary annular screen is configured to capture fibrous material while allowing non-fibrous material and liquid to pass through.

19. The method of claim 1 , wherein the centrifuge further comprises an end cap on the chamber and a stationary tube that extends through the end cap, wherein the stationary tube is configured for directing said biologic mixture into said chamber and the biologic mixture is introduced into the chamber via the stationary tube.

20. The method of claim 19 , wherein the centrifuge further comprises a sizing helix and a core wire, wherein the sizing helix is at least partially contained within said stationary tube and is rotatable about said core wire.

21. The method of claim 1 , further comprising the step of:
c. ejecting from the chamber through the first port a higher density portion of the biologic mixture that has passed through the annular screen.

22. The method of claim 21 , further comprising the step of:
d. recovering from the chamber a portion of the biologic mixture that has passed through the annular screen and remains within the chamber.

Assignees

• Dsm Ip Assets Bv

Inventors

• Nash John E
• Fisher William T
• Bradica Gino

Classifications

• C12N2527/00

  Culture process characterised by the use of mechanical forces, e.g. strain, vibration · CPC title

• C12N5/0653

  Adipocytes; Adipose tissue · CPC title

• C12M45/05 Primary

  by centrifugation (centrifuges in general B04B) · CPC title

• C12M33/10 Primary

  by centrifugation (centrifuges in general B04B); Cyclones (cyclones in general B04C) · CPC title

• C12M1/00

  Apparatus for enzymology or microbiology · CPC title