Bioreactor for microalgae

Patent metadata

Field	Value
Publication number	US-10711232-B2
Application number	US-201615559947-A
Country	US
Kind code	B2
Filing date	Mar 18, 2016
Priority date	Mar 20, 2015
Publication date	Jul 14, 2020
Grant date	Jul 14, 2020

Abstract

The invention relates to a bioreactor comprising a tank containing a culture medium in which a cell culture composed of algae cells is dispersed. The cell culture of algae has a concentration greater than 0.1 g/L in the culture medium, and each algal cell has a minimum absorption in a specific range of wavelengths of light. The bioreactor comprises a light source that is capable of emitting incident light in the direction of the tank, 60% of the photons of the incident light having a wavelength which is included in said specific range of wavelengths of light. The invention also relates to the use of the bioreactor for the production of biomass.

First claim

The invention claimed is:

1. A bioreactor comprising a light source and a tank comprising a culture medium in which a cell culture consisting of green algal cells is dispersed, said green algal cell culture having a concentration of greater than 0.1 g/l in the culture medium, each green algal cell having a minimum absorption for light in a range of wavelengths between 500 nm and 600 nm, said light source being configured to emit incident light in the direction of the tank consisting of at least 60% of photons having a wavelength between 500 nm and 600 nm.

2. The bioreactor as claimed in claim 1 , wherein each green algal cell has a minimum absorption for light in a range of wavelengths of between 530 nm and 600 nm.

3. The bioreactor as claimed in claim 1 , wherein the light source consists of at least one light-emitting diode panel, each light-emitting diode panel emitting at least 60% of photons having a wavelength of between 500 nm and 600 nm.

4. The bioreactor as claimed in claim 1 , wherein the light source is composed of sunlight coupled to at least one semi-transparent photovoltaic panel, each semi-transparent photovoltaic panel being able to filter sunlight and only allow passage of incident light consisting of at least 60% of photons having a wavelength of between 500 nm and 600 nm.

5. The bioreactor as claimed in claim 1 , wherein the culture medium comprises an organic carbon source.

6. The bioreactor as claimed in claim 1 , wherein the bioreactor is driven by a supply mode selected from the group consisting of a batch supply mode, a fed-batch supply mode and a continuous supply mode.

7. The bioreactor as claimed in claim 1 , the bioreactor being of an open type.

8. The bioreactor as claimed in claim 1 , the bioreactor being of a closed type and the algal cell culture having a concentration of greater than 0.5 g/l.

9. The bioreactor as claimed in claim 1 , wherein the algal cell culture has a concentration of greater than 1.0 g/l.

10. The bioreactor as claimed in claim 2 , wherein each green algal cell has a minimum absorption in a light-specific range of wavelengths of between 540 nm and 570 nm.

11. The bioreactor as claimed in claim 3 , wherein each light-emitting diode panel emitting at least 60% of photons having a wavelength of between 540 nm and 570 nm.

12. The bioreactor as claimed in claim 4 , wherein each semi-transparent photovoltaic panel being able to filter sunlight and only allow passage of incident light consisting of at least 60% of photons having a wavelength of between 540 nm and 570 nm.

13. A bioreactor comprising a light source and a tank comprising a culture medium in which a cell culture consisting of red algal cells is dispersed, said red algal cell culture having a concentration of greater than 0.1 g/l in the culture medium, each red algal cell having a minimum absorption for light in a range of wavelengths between 580 nm and 650 nm, said light source being configured to emit incident light in the direction of the tank consisting of at least 60% of photons having a wavelength between 580 nm and 650 nm.

14. The bioreactor as claimed in claim 13 , wherein the light source consists of at least one light-emitting diode panel, each light-emitting diode panel emitting at least 60% of photons having a wavelength of between 580 nm and 650 nm.

15. The bioreactor as claimed in claim 13 , wherein the light source is composed of sunlight coupled to at least one semi-transparent photovoltaic panel, each semi-transparent photovoltaic panel being able to filter sunlight and only allow passage of incident light consisting of at least 60% of photons having a wavelength of between 580 nm and 650 nm.

16. A method for producing microalgal biomass comprising:
dispersing a cell culture having algal cells in a tank comprising a culture medium, said algal cell culture having a concentration of greater than 0.1 g/l in the culture medium, each algal cell having a minimum absorption for light in a light-specific range of wavelengths such that the algal cell absorbs fewer photons from light having a wavelength within the light-specific range of wavelengths than from light having a wavelength outside of the light-specific range of wavelengths;
selecting a light source based on said algal cell culture; and
emitting, by the light source, incident light in the direction of the tank, wherein the incident light has at least 60% of photons having a wavelength within the light-specific range of wavelengths for said algal cell culture, and wherein the light-specific range of wavelengths for said algal cell culture is a range of wavelengths between 500 nm and 650 nm.

17. The method as claimed in claim 16 , wherein said algal cell culture is a green algal cell culture and wherein the emitting incident light in the direction of the tank includes emitting incident light consisting of at least 60% of photons having a wavelength of between 500 nm and 600 nm.

18. The method as claimed in claim 16 , further comprising producing biomass from said algal cell culture.

19. The method as claimed in claim 18 , wherein the algal cell culture is selected from the group consisting of Chlorophyceae, Bacillariophyceae, Trebouxiophyceae, and Cyanophyceae.

20. The method as claimed in claim 16 , wherein said algal cell culture is a red algal cell culture and wherein the emitting incident light in the direction of the tank includes emitting incident light consisting of at least 60% of photons having a wavelength of between 580 nm and 650 nm.

21. The method as claimed in claim 17 , wherein the incident light consists of at least 60% of photons having a wavelength of between 540 nm and 570 nm.

Assignees

• Inria Inst Nat Rech Informatique & Automatique
• Centre Nat Rech Scient
• Univ Pierre Et Marie Curie Paris 6

Inventors

• Mairet Francis
• Bernard Olivier
• Sciandra Antoine
• Pruvost Eric
• Combe Charlotte

Classifications

• C12M31/02

  located outside the reactor · CPC title

• C12M41/06

  of illumination · CPC title

• C12M23/22

  Transparent or translucent parts (glassware for laboratory use B01L3/00) · CPC title

• C12M41/10

  Filtering the incident radiation · CPC title

• C12M21/02 Primary

  Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12) · CPC title