Low fiber pennycress meal and methods of making

Patent metadata

Field	Value
Publication number	US-10709151-B2
Application number	US-201816131633-A
Country	US
Kind code	B2
Filing date	Sep 14, 2018
Priority date	Sep 15, 2017
Publication date	Jul 14, 2020
Grant date	Jul 14, 2020

Abstract

Pennycress seed, seed lots, and seed meal having reduced fiber content and improved suitability for use in producing animal feed are provided.

First claim

What is claimed is:

1. Pennycress seed meal comprising an acid detergent fiber (ADF) content of 7% to 25% by dry weight, wherein the seed meal is defatted, and wherein said meal comprises a detectable amount of a polynucleotide comprising at least one loss-of-function mutation in an endogenous wild-type pennycress gene comprising the polynucleotide sequence of SEQ ID NO: 71 or at least one loss-of-function mutation in an allelic variant of the endogenous wild-type pennycress gene having at least 95% sequence identity to SEQ ID NO: 71.

2. The seed meal of claim 1 , wherein said seed meal has a protein content of 30% to 70% by dry weight.

3. The seed meal of claim 1 , wherein said seed meal has an oil content of 0% to 12% by dry weight.

4. The seed meal of claim 1 , wherein said seed meal has a neutral detergent fiber (NDF) content of 10% to 30% by dry weight.

5. The seed meal of claim 1 , wherein said seed meal has a protein content of 30% to 70% by dry weight and an oil content of 0% to 12% by dry weight.

6. The pennycress seed meal of claim 1 , wherein said meal comprises a detectable amount of a polynucleotide comprising at least one loss-of-function mutation in the least one endogenous wild-type pennycress gene comprising the polynucleotide sequence of SEQ ID NO: 71.

7. The pennycress seed meal of claim 1 , wherein the meal exhibits a lighter-color in comparison to a control pennycress seed meal prepared from wild-type pennycress seed.

8. A composition comprising the pennycress seed meal of claim 1 .

9. A seed lot comprising a population of pennycress seeds that comprise an acid detergent fiber (ADF) content of 5% to 20% by dry weight, wherein the population comprises at least 10 seeds comprising said ADF content and wherein said population of pennycress seeds comprise: (i) seeds having at least one loss-of-function mutation in an endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO: 70 or at least one loss-of-function mutation in an allelic variant of the endogenous wild-type pennycress gene encoding a polypeptide having at least 95% sequence identity to SEQ ID NO: 70; or (ii) comprise seeds having at least one transgene that suppresses expression of an endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO: 70 or at least one transgene that suppresses expression of an allelic variant of the endogenous wild-type pennycress gene encoding a polypeptide having at least 95% sequence identity to SEQ ID NO: 70.

10. The seed lot of claim 9 , wherein said seeds have a protein content of 28% to 40% by dry weight.

11. The seed lot of claim 9 , wherein said seeds have an oil content of 30% to 50% by dry weight.

12. The seed lot claim 9 , wherein said seeds have a neutral detergent fiber (NDF) content of 10% to 25% by dry weight.

13. The seed lot of claim 9 , wherein said seeds have a protein content of 28% to 40% by dry weight and an oil content of 30% to 50% by dry weight.

14. The seed lot of claim 9 , wherein the population comprises at least 500 seeds comprising said ADF content.

15. The seed lot of claim 9 , wherein at least 95% of the pennycress seeds in the seed lot are seeds comprising said ADF content.

16. The seed lot of claim 9 , wherein said seeds further comprise an agriculturally acceptable excipient or adjuvant.

17. The seed lot of claim 9 , wherein said seeds further comprise a fungicide, a safener, or any combination thereof.

18. The seed lot of claim 9 , wherein said population of pennycress seeds comprise seeds having at least one loss-of-function mutation the one endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO: 70 or comprise seeds having at least one transgene that suppresses expression of at least one endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO: 70.

19. The seed lot of claim 9 , wherein the seeds in the population exhibit a lighter-colored seed coat in comparison to a wild-type pennycress seed.

20. A method of making defatted pennycress seed meal comprising an acid detergent fiber (ADF) content of 7% to 25% by dry weight, comprising solvent extracting the seed lot of claim 9 and separating the extracted seed meal from the solvent, thereby obtaining the defatted pennycress seed meal.

21. The pennycress seed meal of claim 1 , wherein said meal comprises an acid detergent fiber (ADF) content of 8% to 20% by dry weight and a detectable amount of the polynucleotide comprising at least one loss-of-function mutation in the endogenous wild-type pennycress gene comprising the polynucleotide sequence of SEQ ID NO: 71 or comprises at least one loss-of-function mutation in an allelic variant of the endogenous wild-type pennycress gene having at least 99% sequence identity to SEQ ID NO: 71.

22. The pennycress seed meal of claim 1 , wherein said meal comprises an acid detergent fiber (ADF) content of 8% to 20% by dry weight and a detectable amount of the polynucleotide comprising at least one loss-of-function mutation in the endogenous wild-type pennycress gene comprising the polynucleotide sequence of SEQ ID NO: 71.

23. The seed lot of claim 9 , wherein the population of pennycress seeds comprise: (i) seeds having at least one loss-of-function mutation in the endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO: 70 or at least one loss-of-function mutation in an allelic variant of the endogenous wild-type pennycress gene encoding a polypeptide having at least 99% sequence identity to SEQ ID NO: 70; or (ii) comprise seeds having at least one transgene that suppresses expression of the endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO: 70 or that suppresses expression of an allelic variant of the endogenous wild-type pennycress gene encoding a polypeptide having at least 99% sequence identity to SEQ ID NO: 70.

24. The seed lot of claim 9 , wherein the population of pennycress seeds comprise: (i) seeds having at least one loss-of-function mutation the endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO: 70; or (ii) comprise seeds having at least one transgene that suppresses expression of the endogenous wild-type pennycress gene encoding the polypeptide of SEQ ID NO: 70.

Assignees

• Covercress Inc
• Board Of Trustees Of Illinois State Univ
• Univ Minnesota

Inventors

• Ulmasov Tim
• Hartnell Gary
• Sedbrook John C
• Marks Michael David
• Chopra Ratan
• Esfahanian Maliheh

Classifications

• A23K10/37 Primary

  from waste material (from hydrolysates of wood or straw A23K10/32; from molasses A23K10/33) · CPC title

• Y02P60/87

  Re-use of by-products of food processing for fodder production · CPC title

• A23K10/30 Primary

  from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms (obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10) · CPC title

• A23K20/158

  Fatty acids; Fats; Products containing oils or fats · CPC title

• A23K50/30

  for swines · CPC title