Method for adjusting the intensity of a light beam in an optical arrangement and associated optical arrangement

Patent metadata

Field	Value
Publication number	US-10705356-B2
Application number	US-201715611802-A
Country	US
Kind code	B2
Filing date	Jun 2, 2017
Priority date	Jun 3, 2016
Publication date	Jul 7, 2020
Grant date	Jul 7, 2020

Abstract

A method for adjusting an intensity of a light beam in an optical arrangement includes passing the light beam through an acousto-optical tunable filter (AOTF). The intensity of the light beam is adjusted as a function of frequency and/or amplitude of a sound wave with which the AOTF is operated. The amplitude of the sound wave at a specified sound wave frequency is selected such that the amplitude is larger than would be required to achieve a first maximum diffraction efficiency for a specified wavelength or for a specified wavelength spectrum of the light beam. The amplitude of the sound wave is also selected such that a value of an integral of a product of the transmission function of the AOTF and the wavelength spectrum of the light beam is larger than at a value of the amplitude to be selected to achieve the first maximum.

First claim

What is claimed is:

1. A method for adjusting an intensity of a light beam in an optical arrangement, the method comprising:
passing the light beam through an acousto-optical tunable filter (AOTF); and
adjusting the intensity of the light beam as a function of at least one of frequency or amplitude of a sound wave with which the AOTF is operated,
wherein the amplitude of the sound wave at a specified sound wave frequency is selected such that the amplitude is greater than would be required to achieve a first peak intensity for a specified wavelength or for a specified wavelength spectrum of the light beam,
wherein the amplitude of the sound wave is also selected such that a value of an integral of a product of a transmission function of the AOTF and the wavelength spectrum of the light beam is greater than a value of an integral of a product of the transmission function of the AOTF and the wavelength spectrum of the light beam at the amplitude to be selected to achieve the first peak intensity, and
wherein a light source or light sources generating the light beam is or are selected or adjusted such that the wavelength spectrum of the light beam is continuous and wider than or as wide as the width of the transmission function of the AOTF.

2. The method according to claim 1 , wherein the selected amplitude is more than twice as large as the amplitude to be selected to achieve the first maximum.

3. The method according to claim 1 , wherein the selected amplitude is less than or equal to three times the amplitude to be selected to achieve the first maximum.

4. The method according to claim 1 , wherein the light beam has a pre-definable wavelength bandwidth.

5. The method according to claim 1 , wherein the light beam is provided using a white light laser or a diode laser.

6. The method according to claim 1 , wherein the light beam is provided as a unified light beam by a plurality of individual lasers.

7. The method according to claim 1 , wherein the intensity of the light beam is adjusted as a function of the amplitude of the sound wave with which the AOTF is operated, and is not adjusted as a function of the frequency of the sound wave with which the AOTF is operated.

8. An optical arrangement, comprising an acousto-optical tunable filter (AOTF) disposed so as to have a light beam passed therethrough, the optical arrangement being configured to adjust an intensity of the light beam as a function of at least one of frequency or amplitude of a sound wave with which the AOTF is operated, wherein the amplitude of the sound wave at a specified sound wave frequency is selected such that the amplitude is greater than would be required to achieve a first peak intensity for a specified wavelength or for a specified wavelength spectrum of the light beam, wherein the amplitude of the sound wave is also selected such that a value of an integral of a product of a transmission function of the AOTF and the wavelength spectrum of the light beam is greater than a value of an integral of a product of the transmission function of the AOTF and the wavelength spectrum of the light beam at the amplitude to be selected to achieve the first peak intensity, and wherein a light source or light sources generating the light beam is or are selected or adjusted such that the wavelength spectrum of the generated light beam is continuous and wider than or as wide as the width of the transmission function of the AOTF.

9. The optical arrangement according to claim 8 , wherein the optical arrangement is integrated in a microscope, and wherein a notch filter is arranged in a detection beam path so as to block an excitation light.

10. The optical arrangement according to claim 8 , wherein the optical arrangement is integrated in a microscope, wherein an acousto-optical beam splitter (AOBS) is part of the optical arrangement in a two-crystal arrangement, a second crystal of the AOBS being operable with an amplitude selected to be sufficiently large so as to diffract excitation light reflected by a specimen from a detection beam path in the same way as the AOTF.

Assignees

• Leica Microsystem Cms Gmbh
• Leica Microsystems

Inventors

• Kremer Manuel
• Krishnamachari Vishnu Vardhan
• Giske Arnold

Classifications

• G02F1/113 Primary

  Circuit or control arrangements · CPC title

• G02F1/116 Primary

  using an optically anisotropic medium, wherein the incident and the diffracted light waves have different polarizations, e.g. acousto-optic tunable filter [AOTF] (G02F1/125 takes precedence) · CPC title

• G02B21/0032

  Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers (G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06) · CPC title

• G02B21/06

  Means for illuminating specimens · CPC title