Stilbenoid prenyltransferases from plants

Patent metadata

Field	Value
Publication number	US-10704052-B2
Application number	US-201715625450-A
Country	US
Kind code	B2
Filing date	Jun 16, 2017
Priority date	Jun 16, 2016
Publication date	Jul 7, 2020
Grant date	Jul 7, 2020

Abstract

The process and system led to the identification of prenyltransferase genes from elicitor-treated peanut hairy roots. One of the prenyltransferases, AhR4DT-1 catalyzes a key reaction involved in the biosynthesis of prenylated stilbenoids, in which resveratrol is prenylated at its C-4 position to form arachidin-2, while another, AhR3′DT-1, was able to add the prenyl group to C-3′ of resveratrol. Each of these prenyltransferases has a high specificity for stilbenoid substrates, and their subcellular location in the plastid was confirmed by fluorescence microscopy. Structure analysis of the prenylated stilbenoids suggest that these two prenyltransferase activities represent the first committed steps in the biosynthesis of a large number of prenylated stilbenoids and their derivatives in peanut.

First claim

What is claimed is:

1. A method of producing a prenylated stilbenoid in a transgenic organism, cell or tissue, the method comprising:
generating a transgenic organism, cell, or tissue comprising a transgene encoding
a polypeptide comprising an amino acid sequence comprising the sequence as set forth in SEQ ID NO: 2 having stilbenoid prenyltransferase activity, and expressing the polypeptide.

2. A method of producing a prenylated stilbenoid in a transgenic organism, cell or tissue, the method comprising:
generating a transgenic organism, cell, or tissue comprising a transgene comprising
a nucleic acid molecule comprising the sequence as set forth in SEQ ID NO: 3 that encodes a polypeptide having stilbenoid prenyltransferase activity, and expressing the nucleic acid molecule.

Assignees

• Arkansas State University—Jonesboro
• Univ Indiana Res & Tech Corp

Inventors

• Medina-Bolivar Luis Fabricio
• Yang Tianhong
• Mockaitis Keithanne

Classifications

• C12N15/81

  for yeasts · CPC title

• C12N15/8243 Primary

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title

• C12N9/1085 Primary

  transferring alkyl or aryl groups other than methyl groups (2.5) · CPC title

• C12Y205/01

  transferring alkyl or aryl groups, other than methyl groups (2.5.1) · CPC title