Liquid-sealed cartridge, specimen analyzer, and specimen analysis method

Patent metadata

Field	Value
Publication number	US-10697989-B2
Application number	US-201715796224-A
Country	US
Kind code	B2
Filing date	Oct 27, 2017
Priority date	Apr 30, 2015
Publication date	Jun 30, 2020
Grant date	Jun 30, 2020

Abstract

This liquid-sealed cartridge includes: a cartridge body including a plurality of liquid storage portions; a seal body configured to seal an opening of each of the plurality of liquid storage portions; and an elastic body disposed so as to be opposed to the plurality of liquid storage portions, the elastic body being configured to form a passage adjacent to the plurality of liquid storage portions. Each of the plurality of liquid storage portions and the passage are configured to be communicated with each other by the corresponding seal body being pushed to be opened through the elastic body.

First claim

What is claimed is:

1. A liquid-sealed cartridge comprising:
a cartridge body including a plurality of liquid storage portions, wherein each liquid storage portion is provided with a first opening and a second opening;
a first seal body configured to seal the first opening of each of the plurality of liquid storage portions and provided integrally with the cartridge body;
a second seal body configured to seal the second opening of each of the plurality of liquid storage portions and provided integrally with the cartridge body; and
an elastic body disposed so as to be distanced above and opposed to the plurality of liquid storage portions, wherein
a passage is provided between the liquid storage portions and the elastic body, and
each of the plurality of liquid storage portions and the passage are configured to be communicated with each other, by the corresponding seal bodies being pushed to be opened through the elastic body.

2. The liquid-sealed cartridge of claim 1 , wherein
each of the first seal body and the second seal body, in a sealed state, is opposed to a lower face of the elastic body forming the passage and is disposed at the opening.

3. The liquid-sealed cartridge of claim 2 , wherein
each of the first seal body and the second seal body includes: a thick portion; and a thin portion connecting an outer peripheral face of the thick portion and an inner peripheral face of the opening to each other.

4. The liquid-sealed cartridge of claim 3 , wherein
each of the first seal body and the second seal body is configured to be disposed in the liquid storage portion after the seal body is opened.

5. The liquid-sealed cartridge of claim 3 , wherein
the thin portion is formed such that a thickness thereof is gradually reduced.

6. The liquid-sealed cartridge of claim 3 , wherein
in the thin portion, a recess having a V-shape in cross section is formed.

7. The liquid-sealed cartridge of claim 1 , wherein
the cartridge body and each of the first seal body and the second seal body provided above the corresponding liquid storage portion are formed from an identical hard material.

8. The liquid-sealed cartridge of claim 1 , wherein
the passage includes a first passage and a second passage that are provided so as to be separated from each other,
the first opening is configured to be communicated with the first passage by the first seal body being pushed to be opened through the elastic body, and
the second opening is configured to be communicated with the second passage by the second seal body being pushed to be opened through the elastic body.

9. The liquid-sealed cartridge of claim 1 , wherein
each of the first seal body and the second seal body is in a shape of a spherical bead, a columnar rod, or a flat plate.

10. The liquid-sealed cartridge of claim 1 , wherein
the plurality of liquid storage portions each include at least one guide for positioning each of the first seal body and the second seal body having been opened.

11. The liquid-sealed cartridge of claim 10 , wherein
each of the first seal body and the second seal body is fixed in a state of being opened as a result of being pushed along the at least one guide.

12. The liquid-sealed cartridge of claim 10 , wherein
the at least one guide includes a holder configured to hold at least one of the first seal body and the second seal body having been opened.

13. The liquid-sealed cartridge of claim 12 , wherein
the at least one guide further includes an inclined portion configured to guide at least one of the first seal body and the second seal body to the holder.

14. The liquid-sealed cartridge of claim 12 , wherein
The at least one guide is composed of a plurality of guide members.

15. The liquid-sealed cartridge of claim 1 , wherein
the plurality of liquid storage portions each include an adhesive film disposed at an inner bottom face thereof, and
each of the first seal body and the second seal body is pushed until coming into contact with the adhesive film, to be opened and fixed.

16. The liquid-sealed cartridge of claim 1 , wherein
the plurality of liquid storage portions each include, at a bottom face side thereof, an air region configured to push back a liquid to the liquid storage portion side.

17. The liquid-sealed cartridge of claim 16 , wherein
the air region is configured to be disposed in a vicinity of a heat block configured to heat the liquid-sealed cartridge.

18. The liquid-sealed cartridge of claim 1 , wherein
the plurality of liquid storage portions each include, at a bottom face side thereof:
a liquid-sending path configured to push out a liquid to the passage side; and
a liquid-sending chamber communicated with the liquid-sending path.

19. A specimen analysis method comprising:
storing a specimen in a liquid-sealed cartridge,
the liquid-sealed cartridge including a first seal body configured to seal a first opening of each of a plurality of liquid storage portions included in a cartridge body, a second seal body configured to seal a second opening of each of the plurality of liquid storage portions included in the cartridge body, the first seal body and the second seal body being provided integrally with the cartridge body, and an elastic body disposed so as to be distanced above and opposed to the plurality of liquid storage portions, wherein a passage is provided between the liquid storage portions and the elastic body; and

pushing and opening each of the first seal body and the second seal body through the elastic body in order to cause each of the plurality of liquid storage portions and the passage to be communicated with each other, and performing analysis of the specimen by use of liquid.

Assignees

• Sysmex Corp
• Asti Corp

Inventors

• Horii Kazuyoshi
• Fujiwara Takao
• OGAI Noriyuki
• Onozuka Kenpei
• Toda Yasuhiro

Classifications

• B01L3/502738 Primary

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title

• B01L2400/0683

  mechanically breaking a wall or membrane within a channel or chamber · CPC title

• B01L2300/049

  Valves integrated in closure · CPC title

• B01L2300/087

  Multiple sequential chambers · CPC title

• B01L2300/123

  Flexible; Elastomeric · CPC title