Method of diagnosis and prognosis

Patent metadata

Field	Value
Publication number	US-10697019-B2
Application number	US-201214113518-A
Country	US
Kind code	B2
Filing date	Apr 27, 2012
Priority date	Apr 27, 2011
Publication date	Jun 30, 2020
Grant date	Jun 30, 2020

Abstract

A method of measuring Gadd45β expression comprising the step of measuring Gadd45β expression levels in a sample of cells, for example CD 138 expressing cells, obtained from a subject known to have or suspected of having a haematological malignancy. Expression levels may be used in diagnosis, for example of multiple myeloma, in providing a prognosis, for example in a patient having multiple myeloma or in guiding selection of an appropriate treatment agent, especially a treatment agent comprising Gadd45β and or MKK7 inhibitors. Also datasets comprising measured expression levels from multiple subjects.

First claim

The invention claimed is:

1. A method of treating a hematological malignancy in a subject in need thereof, the method comprising administering a Gadd45β inhibitor to the subject, wherein the Gadd45β expression level in a sample of CD138 positive cells from the subject is higher than in HEK-293T cells cultured in complete RPMI-1640 medium, and wherein the Gadd45β inhibitor is mDTP3.

2. The method of claim 1 , wherein the haematological malignancy is multiple myeloma.

3. The method of claim 1 , wherein the haematological malignancy is diffuse large B-cell lymphoma.

4. The method of claim 1 , wherein the sample of CD138 positive cells is a blood sample, or biopsy of bone marrow, lymph node, kidney, spleen or bone.

5. The method of claim 2 , wherein said Gadd45β expression levels is a mRNA levels as measured by RT-PCR or other nucleic acid amplification techniques or by a quantitative or semi-quantitative hybridisation technique.

6. The method of claim 2 , wherein said Gadd45β expression level is a protein levels as measured by flow cytometry, Western blotting, RIA, ELISA or other immunoassay.

Assignees

• Franzoso Guido
• Tornatore Laura
• Ruvo Menotti
• Rocci Alberto
• Palumbo Antonio
• Imperial College Innovations Ltd

Inventors

• Franzoso Guido
• Tornatore Laura
• Ruvo Menotti
• Rocci Alberto
• Palumbo Antonio

Classifications

• C12Q2600/118

  Prognosis of disease development · CPC title

• C12Q2600/158

  Expression markers · CPC title

• C12Q1/6886 Primary

  for cancer (immunoassay for cancer G01N33/575) · CPC title