Methods for analyzing nucleic acids from single cells

Patent metadata

Field	Value
Publication number	US-10697013-B1
Application number	US-202016817461-A
Country	US
Kind code	B1
Filing date	Mar 12, 2020
Priority date	Aug 20, 2009
Publication date	Jun 30, 2020
Grant date	Jun 30, 2020

Abstract

Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.

First claim

What is claimed:

1. A method for multiplexed analysis of nucleic acids from single cells, the method comprising:
(a) providing a sample comprising a plurality of cells, wherein a single cell of the plurality of cells comprises a plurality of sample polynucleotides;
(b) performing combinatorial tagging to generate a plurality of tagged polynucleotides from said plurality of sample polynucleotides and a plurality of oligonucleotide tags, wherein a tagged polynucleotide of the plurality of tagged polynucleotides is generated by:
(A) providing an extension product by primer extension using a first oligonucleotide tag and a sample polynucleotide of said plurality of sample polynucleotides, and
(B) ligating a second oligonucleotide tag to said extension product, and

 wherein said tagged polynucleotide of the plurality of tagged polynucleotides comprises:
 (i) a sample sequence corresponding to said sample polynucleotide of the plurality of sample polynucleotides;
 (ii) a first tag sequence distinguishing said sample polynucleotide from sample polynucleotides from other cells; and
 (iii) a second tag sequence distinguishing said sample polynucleotide from other sample polynucleotides from said cell;
(c) amplifying said tagged polynucleotide, thereby generating a plurality of amplified polynucleotides corresponding to the tagged polynucleotide; and
(d) sequencing said plurality of amplified polynucleotides to determine sequences of the amplified polynucleotides corresponding to the sample sequence, the first tag sequence, and the second tag sequence of the tagged polynucleotide; and
(e) using the sequences determined in step (d) to count sample polynucleotides for multiple different sample polynucleotides of multiple different single cells of said plurality of cells.

2. The method of claim 1 , wherein said plurality of sample polynucleotides comprise messenger ribonucleic acid (mRNA).

3. The method of claim 2 , wherein step (A) comprises (i) hybridizing said first oligonucleotide tag to said mRNA and (ii) extending said first oligonucleotide tag using said mRNA as a template to generate complementary DNA (cDNA).

4. The method of claim 3 , wherein step (B) comprises (i) ligating said second oligonucleotide tag to said cDNA.

5. The method of claim 3 , wherein said hybridizing comprises hybridizing a poly-dT sequence of said first oligonucleotide tag to a poly-dA sequence of said mRNA.

6. The method of claim 1 , wherein said plurality of cells is provided in a plurality of wells.

7. The method of claim 1 , wherein said first oligonucleotide tag comprises a sequence that is configured to hybridize to said sample polynucleotides.

8. The method of claim 1 , wherein step (A) comprises (i) hybridizing said first oligonucleotide tag to said sample polynucleotide and (ii) extending said first oligonucleotide tag or said sample polynucleotide or both to provide said extension product.

9. The method of claim 1 , wherein step (b) comprises randomly associating said plurality of sample polynucleotides with said plurality of oligonucleotide tags.

10. The method of claim 1 , wherein said second tag sequences of said plurality of oligonucleotide tags are random sequences.

11. The method of claim 1 , wherein step (c) comprises generating said plurality of amplified polynucleotides using polymerase chain reaction (PCR).

12. The method of claim 1 , wherein substantially every sample polynucleotide of said plurality of sample polynucleotides is associated with the same first tag sequence.

13. The method of claim 1 , wherein at least 90 percent of said plurality of tagged polynucleotides have a second tag sequence that is different from second tag sequences of the other tagged polynucleotides.

14. The method of claim 1 , wherein substantially every tagged polynucleotide of said plurality of tagged polynucleotides has a second tag sequence that is different from second tag sequences of the other tagged polynucleotides.

15. The method of claim 1 , wherein said plurality of oligonucleotide tags comprises a number of different second tag sequences that is larger than the number of sample polynucleotides.

16. The method of claim 15 , wherein said plurality of oligonucleotide tags comprises a number of different second tag sequences that is at least ten times the number of sample polynucleotides.

17. The method of claim 16 , wherein said plurality of oligonucleotide tags comprises a number of different second tag sequences that is at least one hundred times the number of sample polynucleotides.

18. The method of claim 1 , wherein said plurality of oligonucleotide tags comprises at least 200,000 different second tag sequences.

19. The method of claim 1 , wherein an oligonucleotide tag of said plurality of oligonucleotide tags comprises a sequencing adaptor.

20. The method of claim 19 , wherein step (d) comprises hybridizing said amplified polynucleotides or derivatives thereof to a solid support via said sequencing adaptor or derivative thereof.

21. The method of claim 20 , wherein said solid support is a bead.

22. The method of claim 1 , wherein step (e) comprises
(i) using second tag sequences of said plurality of amplified polynucleotides to determine that said plurality of amplified polynucleotides are amplified from said tagged polynucleotide and/or
(ii) using sample sequences, first tag sequences, and second tag sequences of said plurality of amplified polynucleotides to determine that said plurality of amplified polynucleotides are amplified from said tagged polynucleotide.

23. The method of claim 1 , wherein step (e) comprises
(i) determining the number of different second tags sequences associated with said sample sequence, thereby estimating the number of sample polynucleotides having said sample sequence from said single cell and/or (ii) using second tag sequences of said plurality of amplified polynucleotides to provide a digital count of said sample polynucleotides.

24. The method of claim 1 , wherein said plurality of sample polynucleotides are pooled with sample polynucleotides from other single cells of said plurality of cells prior to the generating of step (b).

25. The method of claim 1 , wherein said plurality of tagged polynucleotides are pooled with tagged polynucleotides from other single cells of said plurality of cells prior to the amplifying of step (c).

26. The method of claim 1 , wherein said plurality of amplified polynucleotides are pooled with amplified polynucleotides from other single cells of said plurality of cells prior to the sequencing of step (d).

27. The method of claim 1 , wherein step (e) comprises using first tag sequences of said plurality of amplified polynucleotides to correlate the sample sequences of said plurality of amplified polynucleotides with the single cell from which the sample sequences are derived based on amplified polynucleotides from the same cell having the same first tag sequence.

28. The method of claim 1 , wherein step (e) comprises using second tag sequences of said plurality of amplified polynucleotides to correlate the sample sequences of said plurality of amplified polynucleotides with the sample polynucleotide from which the sample sequences are derived based on amplified polynucleotides from the same sample polynucleotide having the same second tag sequence.

Assignees

• 10X Genomics Inc

Inventors

• Brenner Sydney
• Mikawa Gi
• Osborne Robert
• Slatter Andrew

Classifications

• C12Q1/6855 Primary

  Ligating adaptors · CPC title

• C12N15/1065

  Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags · CPC title

• C12Q1/686

  Polymerase chain reaction [PCR] · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• C12Q1/6874 Primary

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title