Secretory IgA compositions, methods of making and methods of use thereof

Patent metadata

Field	Value
Publication number	US-10696734-B2
Application number	US-201615249811-A
Country	US
Kind code	B2
Filing date	Aug 29, 2016
Priority date	Jan 4, 2013
Publication date	Jun 30, 2020
Grant date	Jun 30, 2020

Abstract

Described herein is secretory IgA isolated from the intestinal luminal fluid and intestinal mucosal of animals such as pigs and cows. Also included are methods of isolating secretory IgA. The secretory IgA is useful in food compositions such as animal and human food compositions as well as pharmaceutical compositions to increase growth rate, improve feed efficiency, reduce gastrointestinal inflammation, reduce a risk of gastrointestinal infection in the animal, or a combination thereof.

First claim

The invention claimed is:

1. A composition comprising
pure, pasteurized secretory IgA isolated from intestinal luminal fluid or intestinal mucosa of a pig or a cow, wherein the pure, pasteurized SIgA composition has an E. coli 055:B5 lipopolysaccharide binding activity at least 5 times greater than pig or cow serum IgA E. coli 055:B5 lipopolysaccharide binding activity.

2. The composition of claim 1 , wherein the pure secretory IgA is greater than 75% pure.

3. The composition of claim 1 , in the form of a pharmaceutical composition further comprising a pharmaceutically acceptable excipient.

4. A composition comprising
secretory IgA isolated from a source that is intestinal mucosa of a pig or a cow, wherein the concentration of secretory IgA in the isolated secretory IgA is at least twice the concentration in the source material, wherein the source material is dried intestinal mucosa, an aqueous preparation from intestinal mucosa, or a dry preparation of proteins prepared from intestinal mucosa, wherein the secretory IgA is pasteurized, wherein the pure, pasteurized SIgA composition has an E. coli 055:B5 lipopolysaccharide binding activity at least 5 times greater than pig or cow serum IgA E. coli 055:B5 lipopolysaccharide binding activity.

5. The composition of claim 4 , wherein greater than 5% of the protein in the isolated secretory IgA comprises IgA.

6. The method of claim 1 , wherein pasteurization is at 50-100° C. for 30 seconds.

7. The method of claim 1 , wherein pasteurization is at 120-150° C. for 1-8 seconds.

8. The method of claim 4 , wherein pasteurization is at 50-100° C. for 30 seconds.

9. The method of claim 4 , wherein pasteurization is at 120-150° C. for 1-8 seconds.

Assignees

• Wisconsin Alumni Res Found
• Wisconsin Alumni Researcg Found

Inventors

• Sand Jordan Marshall
• Cook Mark Eric

Classifications

• A23K50/75

  for poultry · CPC title

• C07K16/18

  against material from animals or humans · CPC title

• A23K50/80

  for aquatic animals, e.g. fish, crustaceans or molluscs · CPC title

• C07K2317/20

  characterized by taxonomic origin · CPC title

• A23K50/20

  for horses · CPC title