Compositions and methods for indirectly reducing incidence of fungal pathogen activity in plants

Patent metadata

Field	Value
Publication number	US-10694751-B2
Application number	US-201916567597-A
Country	US
Kind code	B2
Filing date	Sep 11, 2019
Priority date	Sep 13, 2018
Publication date	Jun 30, 2020
Grant date	Jun 30, 2020

Abstract

Compositions and methods for indirectly reducing incidence of fungal pathogen infection in a plant are disclosed, wherein the fungal pathogen may be Botrytis, Macrophomina, Rhizoctonia, Sclerotinia, or Verticillium. The compositions comprise pasteurized microalgae cells in an amount effective to reduce incidence of the fungal pathogen in a plant compared to a substantially identical population of untreated plants. The composition treatment can include at least one microalgae such as Chlorella, Aurantiochytrium acetophilum HS399, Galdieria, Scenedesmus, Haematococcus, Isochrysis, Spirulina or any combination thereof. Where the composition comprises a combination of a first microalgae and a second microalgae, the composition may consist of a ratio of 25:75, 50:50: or 75:25.

First claim

What is claimed is:

1. A method of reducing incidence of fungal pathogen infection in a plant comprising administering to soil in the immediate vicinity of a plant, seedling, or seed an amount of a microalgae composition that is effective to reduce incidence of the fungal pathogen in the plant compared to a substantially identical population of untreated plants, wherein the microalgae composition comprises at least one of pasteurized Chlorella , pasteurized Aurantiochytrium acetophilum HS399 cells, pasteurized Galdieria cells, pasteurized Scenedesmus cells, pasteurized Haematococcus cells, pasteurized Isochrysis cells, and pasteurized Spirulina cells, and wherein the fungal pathogen is one of Botrytis, Macrophomina, Rhizoctonia, Sclerotinia , and Verticillium.

2. The method claim 1 wherein the microalgae composition comprises pasteurized Chlorella cells and pasteurized Aurantiochytrium acetophilum HS399 cells in a ratio of 25:75.

3. The method of claim 1 wherein the microalgae composition is a liquid and is administered at a rate in the range of 0.25-1.0 gallon/acre.

4. The method of claim 3 wherein the microalgae composition comprises between 100 g-400 g per acre of at least one of solid pasteurized Chlorella cells and solid pasteurized Aurantiochytrium acetophilum HS399 cells.

5. The method of claim 1 wherein the microalgae composition is a liquid and is administered at a rate in the range of 0.5% v/v-5.0% v/v.

6. The method of claim 5 wherein the microalgae composition comprises between 2 g-20 g solid microalgae cells/gallon.

7. The method of claim 1 wherein the microalgae composition comprises pasteurized Aurantiochytrium acetophilum HS399 cells and the pasteurized Aurantiochytrium acetophilum HS399 cells comprise approximately 60% lipid.

8. The method of claim 1 wherein the microalgae composition comprises pasteurized Aurantiochytrium acetophilum HS399 cells and the pasteurized Aurantiochytrium acetophilum HS399 cells comprise approximately 45% lipid.

9. The method of claim 1 wherein the microalgae composition comprises pasteurized Aurantiochytrium acetophilum HS399 cells and the pasteurized Aurantiochytrium acetophilum HS399 cells comprise less than 40% lipid.

10. The method of claim 1 wherein microalgae composition comprises pasteurized Aurantiochytrium acetophilum HS399 cells that have been subjected to an extraction process to remove oils from the pasteurized Aurantiochytrium acetophilum HS399 cells.

11. The method of claim 1 wherein the microalgae composition comprises pasteurized Haematococcus cells that have been subjected to an extraction process to remove oils from the pasteurized Haematococcus cells.

12. The method of claim 1 wherein the microalgae composition comprises pasteurized Galdieria cells.

13. The method of claim 1 wherein the microalgae composition comprises pasteurized Scenedesmus cells.

14. The method of claim 1 wherein the microalgae composition comprises pasteurized Haematococcus cells.

15. The method claim 1 wherein the microalgae composition comprises pasteurized Isochrysis cells.

16. The method of claim 1 wherein the microalgae composition comprises pasteurized Spirulina cells.

17. A method of reducing incidence of fungal pathogen infection in a plant comprising administering to soil in the immediate vicinity of a plant, seedling, or seed an amount of a microalgae composition that is effective to reduce incidence of the fungal pathogen in the plant compared to a substantially identical population of untreated plants, wherein the microalgae composition comprises at least one of pasteurized Chlorella , pasteurized Aurantiochytrium cells, pasteurized Galdieria cells, pasteurized Scenedesmus cells, pasteurized Haematococcus cells, pasteurized Isochrysis cells, and pasteurized Spirulina cells, and wherein the fungal pathogen is one of Botrytis, Macrophomina, Rhizoctonia, Sclerotinia , and Verticillium.

18. The method of claim 17 wherein the microalgae composition is a liquid and is administered at a rate in the range of 0.25-1.0 gallon/acre.

19. The method of claim 17 wherein the microalgae composition is a liquid and is administered at a rate in the range of 0.5% v/v-5.0% v/v.

20. The method of claim 17 wherein the microalgae composition comprises between 2 g-20 g solid microalgae cells/gallon.

21. A method of reducing incidence of fungal pathogen infection in a plant comprising administering to soil in the immediate vicinity of a plant, seedling, or seed an amount of a microalgae composition that is effective to reduce incidence of the fungal pathogen in the plant compared to a substantially identical population of untreated plants, wherein the microalgae composition comprises pasteurized Chlorella and at least one of pasteurized Aurantiochytrium cells, pasteurized Galdieria cells, pasteurized Scenedesmus cells, pasteurized Haematococcus cells, pasteurized Isochrysis cells, and pasteurized Spirulina cells, and wherein the fungal pathogen is one of Botrytis, Macrophomina, Rhizoctonia, Sclerotinia , and Verticillium.

22. The method of claim 21 wherein the microalgae composition comprises pasteurized Chlorella cells and pasteurized Galdieria cells.

23. The method of claim 21 wherein the microalgae composition comprises pasteurized Chlorella cells and pasteurized Scenedesmus cells.

24. The method of claim 21 wherein the microalgae composition comprises pasteurized Chlorella cells and pasteurized Haematococcus cells.

25. The method claim 21 wherein the microalgae composition comprises pasteurized Chlorella cells and pasteurized Isochrysis cells.

26. The method of claim 21 wherein the microalgae composition comprises pasteurized Chlorella cells and pasteurized Spirulina cells.

27. The method of claim 21 wherein the microalgae composition comprises pasteurized Chlorella cells and pasteurized Aurantiochytrium cells.

28. The method of claim 27 wherein the pasteurized Aurantiochytrium cells are pasteurized Aurantiochytrium acetophilum HS399 cells.

Assignees

• Heliae Dev Llc

Inventors

• Carney Laura
• Jauregui Edgard
• Miller Michael

Classifications

• A01N63/30 Primary

  Microbial fungi; Substances produced thereby or obtained therefrom · CPC title

• A01N65/03

  Algae · CPC title

• A01N25/02

  containing liquids as carriers, diluents or solvents · CPC title