Analysis method for mycotoxins

Patent metadata

Field	Value
Publication number	US-10690640-B2
Application number	US-201515754420-A
Country	US
Kind code	B2
Filing date	Aug 28, 2015
Priority date	Aug 28, 2015
Publication date	Jun 23, 2020
Grant date	Jun 23, 2020

Abstract

An analysis method for mycotoxins including a separation step, a detection step, and an identification step. In the separation step, each component contained in a liquid sample is separated in a column In the detection step, components separated in the separation step are detected by a PDA and a fluorescence detector. In the identification step, total aflatoxin is identified based on a detection signal from the fluorescence detector, and deoxynivalenol is identified based on a detection signal from the PDA.

First claim

The invention claimed is:

1. An analysis method for mycotoxins comprising:
a separation step of separating, in a column, total aflatoxin, deoxynivalenol, nivaleonol and patulin contained in a liquid sample, by using a mixed liquid of a buffer and an organic solvent including acetonitrile and methanol as a mobile phase, and supplying the mobile phase to the column with a mixing ratio of acetonitrile and methanol in the mobile phase being increased over time;
a detection step of detecting components separated in the separation step by at least two detectors under same analysis condition; and
an identification step of identifying each component based on a detection result of the detection step, wherein
the at least two detectors include a fluorescence detector, and
in the identification step, based on one result of the detection step under the same analysis condition, total aflatoxin is identified based on a detection signal from the fluorescence detector, and deoxynivalenol, nivaleonol and patulin are identified based on a detection signal from a detector other than the fluorescence detector.

2. The analysis method for mycotoxins according to claim 1 , wherein
the at least two detectors include a photodiode array detector, and
in the identification step, deoxynivalenol, nivaleonol and patulin are identified based on a detection signal from the photodiode array detector.

3. The analysis method for mycotoxins according to claim 2 , wherein identification of deoxynivalenol, nivaleonol and patulin is performed by comparing a spectrum showing a relationship between a wavelength and absorbance at each peak of chromatogram based on the detection signal from the photodiode array detector against each spectrum library for deoxynivalenol, nivaleonol and patulin created in advance, and determining the degree of coincidence, and determining based on the determined degree of coincidence.

4. The analysis method for mycotoxins according to claim 1 , wherein, in the detection step, a wavelength of fluorescence to be detected by the fluorescence detector is switched at a timing set in advance.

5. An analysis method for mycotoxins comprising:
a separation step of separating, in a column, each component contained in a liquid sample;
a detection step of detecting components separated in the separation step by at least two detectors under same analysis condition; and
an identification step of identifying each component based on a detection result of the detection step, wherein
the at least two detectors include a fluorescence detector and a photodiode array detector,
in the identification step, based on one result of the detection step under the same analysis condition, total aflatoxin is identified based on a detection signal from the fluorescence detector, and deoxynivalenol, nivaleonol and patulin are identified based on a detection signal from the photodiode array detector, and
identification of deoxynivalenol, nivaleonol and patulin is performed by comparing a spectrum showing a relationship between a wavelength and absorbance at each peak of chromatogram based on the detection signal from the photodiode array detector against each spectrum library for deoxynivalenol, nivaleonol and patulin created in advance, and determining the degree of coincidence, and determining based on the determined degree of coincidence.

Assignees

• Shimadzu Corp

Inventors

• Uchida Azusa
• Iwata Natsuki
• Nakashima Minori

Classifications

• G01N30/78 Primary

  using more than one detector · CPC title

• G01N30/74 Primary

  Optical detectors {(measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J)} · CPC title

• B01J20/287

  Non-polar phases; Reversed phases · CPC title

• B01J20/292

  Liquid sorbents · CPC title

• G01N2030/884

  organic compounds · CPC title