Methods for identifying polymorphisms linked to the PPO11 gene

Patent metadata

Field	Value
Publication number	US-10689714-B2
Application number	US-201816167275-A
Country	US
Kind code	B2
Filing date	Oct 22, 2018
Priority date	Sep 24, 2012
Publication date	Jun 23, 2020
Grant date	Jun 23, 2020

Abstract

The invention provides compositions and methods related to selective inhibition of PPO11 and use for improving shelf life of a plant or parts thereof. In accordance with the invention, for example, compositions for topical application to a plant or part thereof, are provided that can reduce browning of the plant or part thereof to extend shelf life.

First claim

What is claimed is:

1. A method of identifying a lettuce plant that displays reduced browning or increased shelf life comprising:
a) obtaining a sample of nucleic acids from a population of lettuce plants or parts thereof, wherein members of the population vary for expression of a PPO11 gene having at least 95% sequence identity to SEQ ID NO:9;
b) detecting in said nucleic acids at least one polymorphism that is genetically linked to reduced expression of said PPO11 gene relative to members of the population that do not comprise said polymorphism;
c) crossing a lettuce plant comprising said polymorphism with a second lettuce plant;
d) obtaining seeds from said cross; and
e) growing at least one lettuce plant from said seeds, wherein said lettuce plant grown from said seeds comprises said polymorphism, has a reduced expression of PPO11, and has reduced browning or increased shelf life, when compared with a lettuce plant of the same variety that does not comprise said polymorphism.

2. The method of claim 1 , wherein said polymorphism is located in the PPO11 gene having at least 95% sequence identity to SEQ ID NO:9.

3. The method of claim 1 , wherein said polymorphism is located in a region flanking the PPO11 gene, and wherein said region flanking the PPO11 gene:
a) has at least 95% sequence identity to SEQ ID NO:10; or
b) has at least 95% sequence identity to SEQ ID NO:11.

Assignees

• Seminis Vegetable Seeds Inc

Inventors

• Feikert Michele
• Froman Byron
• Garvey Graeme S
• Kelly Leo
• Waycott William

Classifications

• A01N57/16 Primary

  containing heterocyclic radicals · CPC title

• A01N65/12

  Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon · CPC title

• C12N15/8266

  Abscission; Dehiscence; Senescence · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• C12N15/825

  involving pigment biosynthesis · CPC title