Electronic lighting device
US-10533718-B2 · Jan 14, 2020 · US
Method for the fermentative production of L-lysine by modified Corynebacterium glutamicum
US10689677B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-10689677-B2 |
| Application number | US-201916574588-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 18, 2019 |
| Priority date | Sep 26, 2018 |
| Publication date | Jun 23, 2020 |
| Grant date | Jun 23, 2020 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
A method is used for the fermentative production of L-lysine using bacteria of the species Corynebacterium glutamicum , having the ability to excrete L-lysine and containing in their chromosome a mutated NCgl2816 polynucleotide. Further, the method is used for cultivating the bacteria in a suitable medium under suitable conditions, and accumulating said L-lysine in the suitable medium to form an L-lysine containing fermentation broth.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for the fermentative production of L-lysine, comprising: a) providing a bacterium of the species Corynebacterium glutamicum having an ability to excrete L-lysine containing in the bacterium's chromosome a polynucleotide encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:2, wherein an amino acid phenylalanine at position 220 of the amino acid sequence of SEQ ID NO:2 is substituted by a different proteinogenic amino acid, b) cultivating the bacterium in a suitable medium under suitable conditions, and c) accumulating said L-lysine in the medium to form an L-lysine containing fermentation broth. 2. The method of claim 1 , wherein, in the bacterium, the amino acid at position 220 of the amino acid sequence of SEQ ID NO:2 is cysteine. 3. The method of claim 2 , wherein, in the bacterium, the polynucleotide encoding said amino acid sequence comprises a nucleotide sequence of positions 343 to 1641 of SEQ ID NO:1 with nucleotides at positions 1000 to 1002 being tgt or tgc. 4. The method of claim 3 , wherein the nucleotides at positions 1000 to 1002 are tgc. 5. The method of claim 2 , wherein, in the bacterium, the polynucleotide encoding said amino acid sequence comprises a nucleotide sequence of positions 343 to 1644 of SEQ ID NO:1 with nucleotides at positions 1000 to 1002 being tgt or tgc. 6. The method of claim 5 , wherein the nucleotides at positions 1000 to 1002 are tgc. 7. The method of claim 2 , wherein, in the bacterium, the polynucleotide encoding said amino acid sequence comprises a nucleotide sequence of positions 221 to 1644 of SEQ ID NO:1 with nucleotides at positions 1000 to 1002 being tgt or tgc. 8. The method of claim 7 , wherein the nucleotides at positions 1000 to 1002 are tgc. 9. The method as claimed in claim 1 , further comprising manufacturing of an L-lysine containing product from the L-lysine containing fermentation broth. 10. The method as claimed in claim 1 , further comprising extracting or substantially eliminating water from the L-lysine containing fermentation broth. 11. The method of claim 9 , wherein said manufacturing comprises purification.
Assignees
Inventors
Classifications
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US10689677B2 cover?
- A method is used for the fermentative production of L-lysine using bacteria of the species Corynebacterium glutamicum , having the ability to excrete L-lysine and containing in their chromosome a mutated NCgl2816 polynucleotide. Further, the method is used for cultivating the bacteria in a suitable medium under suitable conditions, and accumulating said L-lysine in the suitable medium to form …
- Who is the assignee on this patent?
- Evonik Operations Gmbh
- What technology area does this patent fall under?
- Primary CPC classification C12P13/08. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 23 2020 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 10 related publications on this page (citations in our corpus or others sharing the same primary CPC).