Nucleic acid molecule for conferring insecticidal properties in plants

Patent metadata

Field	Value
Publication number	US-10689664-B2
Application number	US-201615569096-A
Country	US
Kind code	B2
Filing date	Apr 27, 2016
Priority date	Jun 24, 2015
Publication date	Jun 23, 2020
Grant date	Jun 23, 2020

Abstract

The present invention is drawn to a nucleic acid sequence which confers expression of the insecticidal proteins mCry3A and eCry3.1Ab when introduced into a cell.

First claim

What is claimed is:

1. A nucleic acid molecule comprising a nucleic acid sequence that is at least 98% identical to SEQ ID NO: 1 and encodes mCry3A and eCry3.1Ab insecticidal proteins, or the complement thereof.

2. An isolated nucleic acid molecule comprising a nucleic acid sequence that is at least 98% identical to SEQ ID NO: 1 and encodes mCry3A and eCry3.1Ab insecticidal proteins, or the complement thereof.

3. A chimeric nucleic acid molecule comprising the nucleic acid molecule of claim 1 .

4. A recombinant nucleic acid vector comprising the nucleic acid molecule of claim 1 .

5. A transgenic host cell comprising the nucleic acid molecule of claim 1 .

6. A transgenic host cell according to claim 5 , wherein said cell is a bacterial cell or a plant cell.

7. The host cell according to claim 6 , wherein said cell is an Escherichia coli, Bacillus thuringiensis, Bacillus subtilis, Bacillus megaterium, Bacillus cereus, Agrobacterium ssp. or a Pseudomonas ssp. cell.

8. A transgenic plant, plant part, plant tissue, or plant cell culture comprising the transgenic plant cell of claim 6 .

9. The transgenic plant according to claim 8 , wherein said plant is a monocot plant.

10. The transgenic plant according to claim 8 , wherein said plant is a dicot plant.

11. A transgenic plant according to claim 8 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, sunflower, tomato, crucifers, oat, turf grass, pasture grass, peppers, potato, cotton, rice, soybean, sugarcane, sugar beet, tobacco, barley, and oilseed rape.

12. A progeny of any generation of the plant of claim 8 , wherein the progeny comprises a nucleic acid sequence that is at least 98% identical to SEQ ID NO: 1 and encodes mCry3A and eCry3.1Ab insecticidal proteins, or the complement thereof.

13. A propagule from any generation of the transgenic plant of claim 8 , wherein the propagule comprises a nucleic acid sequence that is at least 98% identical to SEQ ID NO: 1 and encodes mCry3A and eCry3.1Ab insecticidal proteins, or the complement thereof.

14. The propagule of claim 13 , further defined as a seed or a cutting.

15. A method of producing a transgenic plant with enhanced insecticidal properties, comprising introducing the nucleic acid molecule of claim 1 into a plant thereby producing a transgenic plant, wherein the nucleic acid molecule expresses effective insect-controlling amounts of protein.

16. A method of producing a transgenic plant with enhanced insecticidal properties, comprising the steps of:
a) providing the nucleic acid molecule of claim 1 ;
b) introducing into a plant, tissue culture, or a plant cell the nucleic acid molecule of step (a) to obtain a transformed plant, transformed tissue culture, or a transformed cell having enhanced insecticidal properties; and
c) growing said transformed plant or regenerating a transformed plant from the transformed tissue culture or transformed plant cell, so a transgenic plant with enhanced insecticidal properties is produced.

17. A method of producing transgenic seed, comprising the steps of:
a) obtaining a fertile transgenic plant according to claim 8 ; and
b) growing said plant under appropriate conditions to produce said transgenic seed.

18. A method of producing progeny of any generation of a fertile transgenic plant with enhanced insecticidal properties, comprising the steps of:
a) obtaining a fertile transgenic plant with enhanced insecticidal properties comprising the nucleic acid molecule of claim 1 ;
b) collecting transgenic seed from said transgenic plant;
c) planting the collected transgenic seed; and
d) growing the progeny transgenic plants from said seed, wherein said progeny has enhanced insecticidal properties relative to a non-transformed plant.

19. A method for producing a transgenic plant with enhanced insecticidal properties, comprising the steps of sexually crossing a first parent plant with a second parent plant, wherein said first or second parent plant is the plant of claim 8 , to produce a first generation progeny plant that comprises a nucleic acid sequence that is at least 98% identical to SEQ ID NO: 1 and encodes mCry3A and eCry3.1Ab insecticidal proteins, or the complement thereof.

20. A method for producing a transgenic plant with enhanced insecticidal properties, comprising the steps of:
a) sexually crossing a first parent plant with a second parent plant, wherein said first or second parent plant is the plant of claim 8 ; and
b) selecting a first generation progeny plant with enhanced insecticidal properties, wherein the selected progeny plant comprises a nucleic acid sequence that is at least 98% identical to SEQ ID NO: 1 and encodes mCry3A and eCry3.1Ab insecticidal proteins, or the complement thereof.

21. The method of claim 20 , further comprising the steps of:
a) selfing the first generation progeny plant, thereby producing a plurality of second generation progeny plants; and
b) selecting from the second generation progeny plants a plant with enhanced insecticidal properties, wherein the selected second generation progeny plants comprise a nucleic acid sequence that is at least 98% identical to SEQ ID NO: 1 and encodes mCry3A and eCry3.1Ab insecticidal proteins, or the complement thereof.

22. The nucleic acid molecule of claim 1 , wherein the nucleic acid sequence comprises SEQ ID NO: 1, or the complement thereof.

Assignees

• Syngenta Participations Ag

Inventors

• Mittendorf Volker
• Conville Jared
• Hipskind John Daniel
• Azhakanandam Kasimalai
• Noe Andrew
• Fei Xiaoyin
• Donohue Kevin V

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• C12N15/8202

  by biological means, e.g. cell mediated or natural vector · CPC title

• C12N5/04

  Plant cells or tissues {(culture media C12N5/0025)} · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title