Fluid retention plates and analysis cartridges

Patent metadata

Field	Value
Publication number	US-10688489-B2
Application number	US-201715427432-A
Country	US
Kind code	B2
Filing date	Feb 8, 2017
Priority date	Jan 31, 2013
Publication date	Jun 23, 2020
Grant date	Jun 23, 2020

Abstract

Fluid storage containers and analysis cartridges for use in assay processes are presented. In addition, systems comprising such storage containers and analysis cartridges and methods of using such containers and cartridges are presented as well. In specific embodiments, fluid storage containers are configured to be coupled to analysis cartridges in a first stage and a second stage.

First claim

We claim:

1. A system comprising:
(a) a first plate comprising:
(i) a first side;
(ii) a second side;
(iii) a sample reservoir;
(iv) a first port;
(v) a channel connecting the sample reservoir and the first port;
(vi) a sample dispensing channel opening onto the second side of the first plate, wherein the sample dispensing channel is in fluid communication with the sample reservoir;

(b) a frangible blister coupled to the first side of the plate and covering the first port;
(c) a removable sample input cap coupled to the first side of the plate and covering the sample reservoir;
(d) a second plate comprising:
(i) a first side;
(ii) a second side;
(iii) a conduit passing through the second plate from the first side of the second plate to the second side of the second plate;

wherein the first side of the second plate is coupled to the second side of the first plate; and
(e) a printed circuit board coupled to the second side of the second plate.

2. The system of claim 1 , wherein the frangible blister contains a lysis buffer.

3. The system of claim 1 , wherein the sample dispensing channel is in fluid communication with the sample reservoir via a sample dispensing gutter.

4. The system of claim 1 , further comprising a gasket disposed between the second side of the first plate and the first side of the second plate.

5. The system of claim 1 , wherein the second plate further comprises a vent extending from the first side of the second plate to the second side of the second plate.

6. The system of claim 1 , wherein the printed circuit board comprises an electrowetting surface.

7. The system of claim 1 , wherein the printed circuit board comprises one or more heating elements.

8. The system of claim 1 , wherein the first side of the second plate is coupled to the second side of the first plate by a plurality of tabs extending from the second side of the first plate and engaged in a plurality of slots in the second plate.

9. A cartridge comprising:
(a) a first plate comprising:
(i) a first side;
(ii) a second side;
(iii) a sample reservoir;
(iv) a first port;
(v) a channel connecting the sample reservoir and the first port;
(vi) a sample dispensing channel opening onto the second side of the first plate, wherein the sample dispensing channel is in fluid communication with the sample reservoir;

(b) a frangible blister coupled to the first side of the plate and covering the first port;
(c) a removable sample input cap coupled to the first side of the plate and covering the sample reservoir;
(d) a second plate comprising:
(i) a first side;
(ii) a second side;
(iii) a conduit passing through the second plate from the first side of the second plate to the second side of the second plate;

wherein the first side of the second plate is coupled to the second side of the first plate; and
(e) an assay surface coupled to the second side of the second plate, where in the assay surface comprises an electrowetting surface and a printed circuit board.

10. The cartridge of claim 9 , wherein the frangible blister contains a lysis buffer.

11. The cartridge of claim 9 , wherein the sample dispensing channel is in fluid communication with the sample reservoir via a sample dispensing gutter.

12. The cartridge of claim 9 , further comprising a gasket disposed between the second side of the first plate and the first side of the second plate.

13. The cartridge of claim 9 , wherein the second plate further comprises a vent extending from the first side of the second plate to the second side of the second plate.

14. The cartridge of claim 9 , wherein the printed circuit board comprises one or more heating elements.

15. The cartridge of claim 9 , wherein the first side of the second plate is coupled to the second side of the first plate by a plurality of tabs extending from the second side of the first plate and engaged in a plurality of slots in the second plate.

Assignees

• Luminex Corp

Inventors

• Johnson Ross
• Collins Chuck

Classifications

• B01L2400/0683

  mechanically breaking a wall or membrane within a channel or chamber · CPC title

• B01L2400/0427

  Electrowetting · CPC title

• B01L2200/16

  Reagents, handling or storing thereof · CPC title

• B01L2200/10

  Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept · CPC title

• B01L2300/087

  Multiple sequential chambers · CPC title