Device for operating magnetic particles

Patent metadata

Field	Value
Publication number	US-10684279-B2
Application number	US-201815891509-A
Country	US
Kind code	B2
Filing date	Feb 8, 2018
Priority date	Feb 9, 2017
Publication date	Jun 16, 2020
Grant date	Jun 16, 2020

Abstract

To provide a device for operating magnetic particles capable of treating large quantity, and retaining a gel-like medium layer stably, a vessel is formed so that an area perpendicular to a longitudinal direction in a large-diameter part packed with a liquid layer is larger than an area perpendicular to the longitudinal direction in the small-diameter part packed with a gel-like medium layer. Therefore, in the vessel, it is possible to reduce the diameter of the small-diameter part while keeping the capacity of the large-diameter part large. As a result, in the vessel, it is possible to treat large quantity in the liquid layer, and it is possible to retain the gel-like medium layer stably.

First claim

What is claimed is:

1. A device for operating magnetic particles, comprising a tubular vessel formed with an internal space where a gel-like medium layer and a liquid layer are alternately stacked in a longitudinal direction, and magnetic particles are packed, wherein
an area of a first cross section that is perpendicular to the longitudinal direction in a part packed with the liquid layer in the internal space of the vessel is larger than an area of a second cross section that is perpendicular to the longitudinal direction in a part packed with the gel-like medium layer.

2. The device for operating magnetic particles according to claim 1 , wherein
the internal space of the vessel contains a sample introduction space and a sample migration space;
in the sample introduction space, a sample containing a target component is introduced, said target component is fixed to the magnetic particles,
in the sample migration space, the gel-like medium layer and the liquid layer are alternately stacked in the longitudinal direction—
in the sample migration space, the target component is migrated in the longitudinal direction while fixed to the magnetic particles,
the first cross section is a section perpendicular to the longitudinal direction of a part packed with the liquid layer in the sample migration space, and
the second cross section is a section perpendicular to the longitudinal direction of a part packed with the gel-like medium layer in the sample migration space.

3. The device for operating magnetic particles according to claim 1 , wherein
in the vessel, a magnetic field application part on one longitudinal surface of the vessel is movable along the longitudinal direction; wherein said one longitudinal surface is opposite an opposing surface; and
in the first cross section, an inner diameter of the opposing surface is larger than an inner diameter in a direction perpendicular to the opposing surface.

4. The device for operating magnetic particles according to claim 3 , wherein a part opposed to the magnetic field application part on the opposing surface side in the first cross section and a part opposed to the magnetic field application part on the opposing surface side in the second cross section are aligned with each other in the longitudinal direction.

5. The device for operating magnetic particles according to claim 1 , wherein the part packed with the liquid layer and the part packed with the gel-like medium layer in the internal space of the vessel are connected with each other by a tapered surface inclined with respect to the longitudinal direction.

6. The device for operating magnetic particles according to claim 1 , wherein
the liquid layer includes a washing layer packed with a washing liquid for washing a target substance in a sample, and an elution layer packed with an eluent for eluting a target component in the sample, and
the first cross section is a section perpendicular to the longitudinal direction of the washing layer in the internal space of the vessel.

Assignees

• Shimadzu Corp

Inventors

• Kanai Masaki
• Jikuya Hiroyuki
• Ohashi Tetsuo
• Fujiwara Seiya

Classifications

• G01N1/34 Primary

  Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)} · CPC title

• G01N2001/4083

  sedimentation · CPC title

• B03C1/12 Primary

  with magnets moving during operation; with movable pole pieces · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• G01N33/54326 Primary

  Magnetic particles · CPC title