Structuring fats and methods of producing structuring fats

Patent metadata

Field	Value
Publication number	US-10683522-B2
Application number	US-201916373496-A
Country	US
Kind code	B2
Filing date	Apr 2, 2019
Priority date	Apr 18, 2012
Publication date	Jun 16, 2020
Grant date	Jun 16, 2020

Abstract

Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

First claim

What is claimed is:

1. A microbial oil comprising triglycerides, wherein the microbial oil has an altered fatty acid profile relative to a microbial oil produced by a non-recombinant oleaginous microbe, the microbial oil comprising at least 45% of triglycerides being a combination of palmitic-oleic-palmitic triglyceride, stearic-oleic-stearic triglyceride, and palmitic-oleic-stearic triglyceride, and wherein the oleaginous microbe comprises an exogenous gene encoding ketoacyl-ACP synthase (KAS).

2. The microbial oil of claim 1 , wherein the ketoacyl-ACP synthase is selected from the group consisting of KASI, KASII, KASIII or KASIV.

3. The microbial oil of claim 2 , wherein the ketoacyl-ACP synthase is KASII.

4. The microbial oil of claim 3 , wherein the KASII has at least 95% sequence identity to a protein encoded by the nucleotide sequence of SEQ ID NO: 95.

5. The microbial oil of claim 1 , wherein the oleaginous microbe further comprises an exogenous gene that encodes a fatty acyl-ACP thioesterase, lysophosphatidic acid acyltransferase (LPAAT), or sucrose invertase.

6. The microbial oil of claim 1 , wherein the oleaginous microbe further comprises nucleic acids that are operable to reduce the expression of stearoyl-ACP desaturase or fatty acid desaturase, or fatty acyl-ACP thioesterase.

7. The microbial oil of claim 5 , wherein the oleaginous microbe further comprises nucleic acids that are operable to reduce the expression of stearoyl-ACP desaturase or fatty acid desaturase, or fatty acyl-ACP thioesterase.

8. The microbial oil of claim 1 , wherein the oleaginous microbe is an oleaginous microalgae.

9. The microbial oil of claim 8 , wherein the oleaginous microalgae is of the genus Prototheca.

10. The microbial oil of claim 9 , wherein the oleaginous microalge is Prototheca moriformis.

Assignees

• Corbion Biotech Inc

Inventors

• Franklin Scott
• Somanchi Aravind
• Rudenko George
• Bhat Riyaz
• Zhao Xinhua
• Bond Risha
• Rakitsky Walter
• Marangoni Alejandro
• Braksmayer Diza

Classifications

• C12R2001/89

  Algae {; Processes using algae} · CPC title

• C12N1/125

  Unicellular algae isolates · CPC title

• Y02E50/10

  Biofuels, e.g. bio-diesel · CPC title

• C12N9/16

  acting on ester bonds (3.1) · CPC title

• C12Y203/0102

  Diacylglycerol O-acyltransferase (2.3.1.20) · CPC title