Liposomes, emulsions, and methods for cryotherapy

Patent metadata

Field	Value
Publication number	US-10682297-B2
Application number	US-201715498867-A
Country	US
Kind code	B2
Filing date	Apr 27, 2017
Priority date	May 10, 2016
Publication date	Jun 16, 2020
Grant date	Jun 16, 2020

Abstract

A method and system in accordance with a particular embodiments of the technology includes applying a substance onto skin of a human subject. The applied substance can include a freezing point depressant and a liposome, an oil-in-water emulsion, a water-in-oil emulsion, or an oil-in-oil emulsion and can be configured to protect or target tissue. The substance and a surface of the skin can be cooled using the applicator to treat acne and other skin conditions.

First claim

What is claimed is:

1. A method for treating a subject's skin, comprising:
applying a coupling media to the skin, wherein the coupling media includes an ice nucleation agent, a freezing point depressant, and either a liposome, an oil-in-water emulsion, a water-in-oil emulsion, or an oil-in-oil emulsion containing the freezing point depressant to enhance delivery of the freezing point depressant to the skin; and
cooling the coupling media and a surface of the skin with an applicator to a temperature below 0° C. to treat the skin.

2. The method of claim 1 , wherein the freezing point depressant comprises propylene glycol, glycerol, and/or polyethylene glycol.

3. The method of claim 1 , wherein the coupling media includes the liposome, and wherein the liposome and freezing point depressant is a propylene glycol liposome.

4. The method of claim 1 , wherein the coupling media includes the liposome, and wherein the liposome further comprises water such that the freezing point depressant and the water are released into the skin after the liposome breaks down.

5. The method of claim 1 , wherein the coupling media includes the liposome, wherein the method further comprises
breaking a lipid encapsulation of the liposome using ultrasound energy, temperature cycling, and/or a cleansing agent to release the freezing point depressant.

6. The method of claim 1 , further comprising controlling cooling of the coupling media and the skin surface so as to not to freeze the epidermis.

7. The method of claim 1 , further comprising controlling cooling of the coupling media and the surface cooling so as to freeze the skin.

8. The method of claim 1 , wherein the coupling media is either the oil-in-water emulsion or the water-in-oil emulsion.

9. The method of claim 8 , wherein the oil-in-water emulsion or the water-in-oil emulsion is a nanoemulsion.

10. A method for treating a subject's skin, comprising:
applying a coupling media to the skin, wherein the coupling media includes an ice nucleation agent, a freezing point depressant, and either a liposome, an oil-in-water emulsion, a water-in-oil emulsion, or an oil-in-oil emulsion containing the freezing point depressant to enhance delivery of the freezing point depressant to the skin;
cooling the coupling media and a surface of the skin with an applicator to a temperature below 0° C. to treat the skin; and
controlling cooling of the coupling media and the surface of the skin to predictably freeze the skin based on nucleation caused by the ice nucleation agent.

11. A method for treating a subject's skin, comprising:
applying a coupling media to a surface of the subject's skin such that an ice nucleating agent contained in the applied coupling media does not directly contact epidermal cells for a period of time;
applying an applicator to the subject's skin; and
cooling the coupling media and the surface of the skin with the applicator to lower a temperature of the surface of the skin below 0° C. to cause a freeze event initiated by the ice nucleating agent so as to freeze the skin.

12. The method of claim 11 , wherein cooling the coupling media and the surface of the skin include removing heat from the skin to start freezing the skin after the period of time.

13. The method of claim 11 , wherein the coupling media comprises a liposome, an oil-in-water emulsion, a water-in-oil emulsion, an oil-in-oil emulsion, and/or nano-emulsion.

14. The method of claim 11 , wherein the coupling media includes a freezing point depressant for lowering a freezing point of the skin.

15. The method of claim 14 , wherein the freezing point depressant is configured to penetrate the skin only a shallow depth to lower a freezing point of epidermal tissue more than dermal tissue.

16. The method of claim 11 , further comprising breaking apart a liposome of the coupling media to release the ice nucleating agent for initiating the freeze event.

17. The method of claim 11 , wherein cooling the coupling media and the surface of the skin includes:
removing heat from the skin to supercool targeted tissue in the skin;
freezing the targeted tissue; and
after freezing the targeted tissue, removing heat from and/or applying heat to the skin to keep an epidermal layer frozen while a dermal layer thaws and refreezes.

18. The method of claim 11 , further comprising either:
diluting the coupling media to raise its freezing point to produce the freeze event, and/or
delivering energy to activate the ice nucleating agent, and/or
reducing the temperature of the coupling media to cause the freeze event after a fluid in the subject's skin has been cooled below its freezing point.

19. A system for treating a subject, comprising:
an applicator configured to reduce a temperature of a target region beneath a surface of the subject's skin to reduce the temperature of target tissue from a natural body temperature to a temperature for freezing the target tissue;
a coupling media including an ice nucleating agent, a freezing point depressant and either a liposome, an oil-in-water emulsion, a water-in-oil emulsion, or oil-in-oil emulsion containing the freezing point depressant to enhance delivery of the freezing point depressant to the skin; and
a controller programmed to cause the applicator to cool the coupling media and a surface of the skin to a temperature below 0° C. to freeze the skin for a period of time.

Assignees

• Zeltiq Aesthetics Inc

Inventors

• Zeng Like
• Jimenez Lozano Joel N
• Debenedictis Leonard C

Classifications

• A61B2090/0463

  against cooling or freezing · CPC title

• A61P17/10

  Anti-acne agents · CPC title

• A61K9/107

  Emulsions {; Emulsion preconcentrates; Micelles (composition of emulsions A61K47/00)} · CPC title

• A61F2007/0076

  remote from the spot to be heated or cooled · CPC title

• A61F2007/0219

  Gels · CPC title