Method and apparatus for improved mass spectrometer operation

Patent metadata

Field	Value
Publication number	US-10679841-B2
Application number	US-201816169900-A
Country	US
Kind code	B2
Filing date	Oct 24, 2018
Priority date	Jun 13, 2018
Publication date	Jun 9, 2020
Grant date	Jun 9, 2020

Abstract

A method of operating a quadrupole mass filter is disclosed. A first set of RF and resolving DC voltages are applied to electrodes of a quadrupole mass filter to selectively transmit first ions having a first mass-to-charge ratio (m/z). A second set of RF and resolving DC voltages are applied to electrodes of the quadrupole mass filter to selectively transmit second ions having a second m/z. Detection of the second ions is initiated after completion of a settling time. The settling time is determined in accordance with the relationship: Eq. 1, where ts is the settling time, (m/z)1 is the first mass-to-charge ratio, (m/z)2 is the second mass-to-charge ratio and A, B and C are empirically derived coefficients.

First claim

What is claimed is:

1. A method of operating a tandem mass spectrometer having a first quadrupole mass filter (Q1), and a second quadrupole mass filter (Q3), and a quadrupole collision cell (Q2) disposed between the first (Q1) and second (Q3) quadrupole mass filters, comprising:
applying a first set of RF and resolving DC voltages to electrodes of the first (Q1) and second (Q3) quadrupole mass filters to cause the first quadrupole mass filter (Q1) to selectively transmit first precursor ions and the second quadrupole mass filter (Q3) to selectively transmit first product ions;
applying a second set of RF and resolving DC voltages to electrodes of the first (Q1) and second (Q3) quadrupole mass filters to cause the first quadrupole mass filter (Q1) to selectively transmit second precursor ions and the third quadrupole mass filter (Q3) to selectively transmit second product ions, wherein at least one of: (i) the first and second precursor ions and (ii) the first and second product ions have mass-to-charge ratios that differ from one another; and
initiating detection of the second ions after completion of a settling time, wherein the settling time for each quadrupole Q1, Q2, and Q3 is determined at accordance with the relationships:
t Q1s =A [( m/z ) 2 −( m/z ) 1 ] B +C √{square root over (( m/z ) 2 )},

t Q2s =C *√{square root over (( m/z ) p2 )},

t Q3s =A [( m/z ) p2 −( m/z ) p1 ] B +C √{square root over (( m/z ) p2 )},

in which t Q1s is the settling time for Q1, t Q2s is the settling time for Q2, t Q3s is the settling time for Q3, (m/z) 1 is the first mass-to-charge ratio, (m/z) 2 is the second mass-to-charge ratio, (m/z) p1 is the first product mass-to-charge ratio, (m/z) p2 is the second product mass-to-charge ratio, and A, B, C and C* are empirically derived coefficients,
wherein the total settling time is determined in accordance with the relationship:
t s-tot =MAX{( t Q1s +t Q2s ), t Q2s }.

2. The method of claim 1 wherein the settling time is determined for each targeted ion transition.

3. The method of claim 1 wherein A and B are electronics settling time coefficients, and C and C* are time of flight (TOF) coefficients.

4. The method of claim 3 wherein the values of A and B are dependent on whether the m/z of a next target ion is greater than or less than the m/z of a previous target ion.

Assignees

• Thermo Finnigan Llc

Inventors

• Oser Harald
• Song Qingyu
• Ugarov Mikhail V

Classifications

• H01J49/4215

  Quadrupole mass filters (H01J49/4225 takes precedence) · CPC title

• H01J49/429 Primary

  Scanning an electric parameter, e.g. voltage amplitude or frequency · CPC title

• H01J49/0031 Primary

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title