Platelet test chip

Patent metadata

Field	Value
Publication number	US-10677779-B2
Application number	US-201615553284-A
Country	US
Kind code	B2
Filing date	Feb 23, 2016
Priority date	Feb 24, 2015
Publication date	Jun 9, 2020
Grant date	Jun 9, 2020

Abstract

Disclosed is a platelet-testing chip. The platelet-testing chip includes a plurality of sample chambers storing blood samples, stirrers provided in the sample chambers to apply shearing force to the blood samples, a plurality of waste sample chambers provided so as to correspond to the plurality of the sample chambers, microchannels, through which the sample chambers and the waste sample chambers corresponding to each other are independently connected to thus form paths through which the blood samples flow from the sample chambers to the corresponding waste sample chambers, and microbeads which are received in one or more of the plurality of the sample chambers and which are coated with a reagent for activating platelets on an outer surface thereof. When the blood samples are transferred from the sample chambers through the microchannels, the microbeads are transferred together with the blood samples.

First claim

The invention claimed is:

1. A platelet-testing chip comprising:
a plurality of sample chambers storing blood samples;
stirrers provided in the sample chambers, respectively, to stir the blood samples;
a plurality of waste sample chambers provided so as to correspond to the plurality of the sample chambers;
microchannels, through which the sample chambers and the waste sample chambers corresponding to each other are independently connected to thus form paths through which the blood samples flow from the sample chambers to the corresponding waste sample chambers;
clogging-induction channel units formed on the paths of the microchannels;
addition chambers formed between the clogging-induction channel units and the plurality of sample chambers and comprising calcium ions or adenosine diphosphates (ADP);
microbeads which are received in one or more of the plurality of the sample chambers and which are coated with a reagent for activating platelets on an outer surface thereof; and
magnetic units applying a magnetic field to the clogging-induction channel units,
wherein when the blood samples are transferred from the sample chambers through the microchannels, the microbeads are transferred together with the blood samples, thus inducing clogging of the clogging-induction channel units with the blood samples,
wherein the platelets of the blood samples are attached to the microbeads and are aggregated in the sample chambers and the microchannels, and then flow into the clogging-induction channel units, thus clogging the clogging-induction channel units, and
wherein the microbeads include a material having magnetism or a magnetizable material so as to be captured in the clogging-induction channel units due to the magnetism of the magnetic units while flowing together with the blood samples, thus impeding or stopping a flow of the blood samples.

2. The platelet-testing chip of claim 1 , wherein a reagent for activating the platelets is applied on inner wall surfaces of the clogging-induction channel units to induce clogging.

3. The platelet-testing chip of claim 2 , wherein the reagent applied on the inner wall surfaces of the clogging-induction channel units is an antagonist comprising at least one of collagen, fibrinogen, adenosine diphosphate (ADP), epinephrine, arachidonic acid, thromboxane A2, thrombin, and iso-thrombin-receptor-activating peptide (iso-TRAP), or a combination thereof.

4. The platelet-testing chip of claim 1 , wherein inner diameters of the clogging-induction channel units are smaller than inner diameters of the microchannels so as to induce clogging.

5. The platelet-testing chip of claim 1 , wherein the reagent applied on the microbeads is an antagonist comprising at least one of collagen, fibrinogen, adenosine diphosphate (ADP), epinephrine, arachidonic acid, thromboxane A2, thrombin, and iso-thrombin-receptor-activating peptide (iso-TRAP), or a combination thereof.

6. The platelet-testing chip of claim 1 , wherein the plurality of the sample chambers includes a standard sample chamber and one or more control sample chambers, and one or more of final reaching distances, reaching times required for the final reaching distances, and flow rates of the blood samples that flow from the standard sample chamber to the corresponding microchannel and of the blood samples that flow from the control sample chambers to the corresponding microchannels are selected, followed by comparison thereof, thus measuring characteristics of the platelets.

7. The platelet-testing chip of claim 6 , further comprising:
a stirring controller individually controlling the stirrers provided in the sample chambers so that magnitudes of shearing forces applied to the blood samples in the sample chambers are individually controlled.

8. The platelet-testing chip of claim 7 , wherein the stirring controller controls the stirrers in standard sample chambers so that the shearing forces having the magnitudes insufficient to activate platelets are applied to the blood samples in the standard sample chambers, and also controls the stirrers in the control sample chambers so that the shearing forces having the magnitudes sufficient to activate the platelets are applied to the blood samples in the control sample chambers, and microbeads received in the standard sample chambers and the control sample chambers are coated with a same reagent so that an extent of reaction of the platelets depending on the shearing forces is identified.

9. The platelet-testing chip of claim 7 , wherein the control sample chambers are provided in a plural number, the stirring controller controls the stirrers in standard sample chambers so that the shearing forces having the magnitudes insufficient to activate the platelets are applied to the blood samples in the standard sample chambers, and also controls the stirrers in the control sample chambers so that the shearing forces having the different magnitudes sufficient to activate the platelets are applied to the blood samples in the control sample chambers in order to determine an extent of activation of the platelets depending on the magnitudes of the shearing forces, and microbeads received in the standard sample chambers and the control sample chambers are coated with a same reagent so that an extent of reaction of the platelets depending on the shearing forces is identified.

10. The platelet-testing chip of claim 7 , wherein the control sample chambers are provided in a plural number, the microbeads are received only in the control sample chambers, the microbeads received in the control sample chambers are coated with different reagents, and the stirring controller applies the shearing forces having the magnitudes insufficient to activate the platelets to the blood samples in standard sample chambers and the control sample chambers.

11. The platelet-testing chip of claim 1 ,
wherein the calcium ions or the adenosine diphosphates (ADP) are transferred together with the blood samples passing through the addition chambers, thus promoting activation of the platelets using a reagent.

Assignees

• Univ Korea Res & Bus Found

Inventors

• Shin Se-Hyun

Classifications

• G01N33/4915 Primary

  using flow cells (flow cytometry G01N15/14) · CPC title

• B01L2300/0867

  Multiple inlets and one sample wells, e.g. mixing, dilution · CPC title

• B01L2300/0883

  Serpentine channels · CPC title

• B01F13/0818

  Operations & Transport · mapped topic

• B01F13/0059

  Operations & Transport · mapped topic