IRDIG17912 insecticidal Cry toxins

Patent metadata

Field	Value
Publication number	US-10676757-B2
Application number	US-201615237918-A
Country	US
Kind code	B2
Filing date	Aug 16, 2016
Priority date	Aug 25, 2015
Publication date	Jun 9, 2020
Grant date	Jun 9, 2020

Abstract

Insecticidal toxins derived from Bacillus thuringiensis, polynucleotides encoding such toxins, use of such toxins to control plant pests, and transgenic plants that produce, and are protected, by these toxins are described.

First claim

The invention claimed is:

1. An engineered chimeric insecticidal toxin selected from the group consisting of SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, and a toxin having at least 95% sequence identity with SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16.

2. A DNA sequence encoding an engineered chimeric insecticidal toxin of claim 1 .

3. A nucleic acid construct comprising a nucleic acid sequence encoding an IRDIG17912 insecticidal toxin of SEQ ID NO: 2 or SEQ ID NO: 4, or a toxin having at least 95% sequence identity with SEQ ID NO: 2 or SEQ ID NO: 4, operably linked to a heterologous promoter capable of driving expression in a plant.

4. A nucleic acid construct comprising the DNA of claim 2 , operably linked to a heterologous-promoter capable of driving expression in a plant.

5. A transgenic plant or plant part comprising the toxin of claim 1 .

6. A transgenic plant or plant part comprising the nucleic acid construct of claim 3 .

7. A transgenic plant or plant part comprising the nucleic acid construct of claim 4 .

8. A method for controlling a western corn rootworm pest population comprising contacting members of said western corn rootworm pest population with the transgenic plant or plant part of claim 6 .

9. A method for controlling a western corn rootworm pest population comprising contacting members of said western corn rootworm pest population with the transgenic plant or plant part of claim 7 .

10. A method for controlling a western corn rootworm pest population comprising contacting members of said western corn rootworm pest population with a pesticidally effective amount of the insecticidal toxin of claim 1 .

11. A Pseudomonas fluorescens host strain engineered to express a DNA encoding an IRDIG17912 insecticidal toxin of SEQ ID NO: 2 or SEQ ID NO: 4, or a toxin having at least 95% sequence identity with SEQ ID NO: 2 or SEQ ID NO: 4.

Assignees

• Dow Agrosciences Llc

Inventors

• Hey Timothy D
• Armstrong Janna Mai
• Letherer Ted
• Etter Audrey Jane
• Frey Meghan L
• Ellis Haley
• Narva Kenneth E
• Xu Xiaoping
• Madduri Krishna M
• Tan Sek Yee
• Gandra Premchand

Classifications

• C07K14/325

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• Y02A40/162

  Cross-Sectional Technologies · mapped topic

• C12N15/8286 Primary

  for insect resistance · CPC title

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title