Microorganism with enhanced L-lysine producibility and method for producing L-lysine using the same

Patent metadata

Field	Value
Publication number	US-10676512-B2
Application number	US-201515508662-A
Country	US
Kind code	B2
Filing date	Sep 4, 2015
Priority date	Sep 5, 2014
Publication date	Jun 9, 2020
Grant date	Jun 9, 2020

Abstract

The present disclosure relates to a novel modified RNA polymerase sigma factor A (SigA) polypeptide; a polynucleotide encoding the same; a microorganism containing the polypeptide; and a method for producing L-lysine using the microorganism.

First claim

The invention claimed is:

1. A modified polypeptide having RNA polymerase sigma factor A activity and the amino acid sequence set forth in SEQ ID NO: 2 but with one or two amino acid substitutions, wherein at least one of the one or two amino acid substitutions is selected from the group consisting of a substitution of the 136 th amino acid with glycine; a substitution of the 254 th amino acid with asparagine; a substitution of the 268 th amino acid with serine; a substitution of the 281 st amino acid with serine; a substitution of the 381 st amino acid with arginine; a substitution of the 429 th amino acid with arginine; a substitution of the 447 th amino acid with histidine; a substitution of the 451 st amino acid with isoleucine; a substitution of the 455 th amino acid with valine; a substitution of the 479 th amino acid with arginine; a substitution of the 483 rd amino acid with arginine; a substitution of the 488 th amino acid with threonine; and a substitution of the 491 st amino acid with arginine.

2. The modified polypeptide according to claim 1 , wherein the modified polypeptide has an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ ID NOS: 12 to 22.

3. A polynucleotide encoding the modified polypeptide of claim 1 .

4. A host cell comprising the polynucleotide of claim 3 .

5. A microorganism of the genus Corynebacterium having enhanced L-lysine producibility, comprising a modified polypeptide having RNA polymerase sigma factor A activity and the amino acid sequence set forth in SEQ ID NO: 2 but with one or two substitutions in SEQ ID NO:2, wherein at least one of the one or two substitutions is selected from the group consisting of a substitution of the 136 th amino acid with glycine; a substitution of the 254 th amino acid with asparagine; a substitution of the 268 th amino acid with serine; a substitution of the 281 st amino acid with serine; a substitution of the 381 st amino acid with arginine; a substitution of the 429 th amino acid with arginine; a substitution of the 447 th amino acid with histidine; a substitution of the 451 st amino acid with isoleucine; a substitution of the 455 th amino acid with valine; a substitution of the 479 th amino acid with arginine; a substitution of the 483 rd amino acid with arginine; a substitution of the 488 th amino acid with threonine; and a substitution of the 491 st amino acid with arginine.

6. The microorganism according to claim 5 , wherein the microorganism is Corynebacterium glutamicum.

7. A method for producing L-lysine, comprising:
(a) culturing the microorganism according to claim 5 in a medium; and
(b) recovering L-lysine from the cultured microorganism or the cultured medium.

Assignees

• Cj Cheiljedang Corp

Inventors

• Huh Lan
• Lee Kwang Ho
• Kim Hyung Joon
• Moon Jun Ok
• Ryu Song-Gi

Classifications

• C07K14/34 Primary

  from Corynebacterium (G) · CPC title

• C12P13/08

  Lysine; Diaminopimelic acid; Threonine; Valine · CPC title

• C12Y207/07006

  DNA-directed RNA polymerase (2.7.7.6) · CPC title

• C12N9/12 Primary

  transferring phosphorus containing groups, e.g. kinases (2.7) · CPC title

• C12R1/15

  Chemistry & Metallurgy · mapped topic