Membrane washing apparatus

Patent metadata

Field	Value
Publication number	US-10670558-B2
Application number	US-201715474652-A
Country	US
Kind code	B2
Filing date	Mar 30, 2017
Priority date	Apr 6, 2016
Publication date	Jun 2, 2020
Grant date	Jun 2, 2020

Abstract

The present disclosure describes an apparatus including a waste compartment, a membrane compartment on the waste compartment, and a wash buffer reservoir on the membrane compartment. The membrane compartment includes a membrane compartment outlet aperture and is configured to hold at least one membrane, and the membrane compartment outlet aperture fluidly connects the membrane compartment and the waste compartment. The wash buffer reservoir comprises a wash buffer reservoir outlet aperture, and the wash buffer reservoir outlet aperture fluidly connects the wash buffer reservoir and the membrane compartment. The apparatus may be used to wash membranes used in analytic techniques, such as membranes for gel electrophoresis.

First claim

What is claimed is:

1. An apparatus comprising:
a waste compartment;
a membrane compartment on the waste compartment, wherein the membrane compartment comprises a membrane compartment outlet aperture and is configured to hold at least one membrane, and wherein the membrane compartment outlet aperture fluidly connects the membrane compartment and the waste compartment; and
a wash buffer reservoir on the membrane compartment, wherein the wash buffer reservoir is configured to contain wash buffer solution, wherein the wash buffer reservoir comprises a wash buffer reservoir outlet aperture, wherein the wash buffer reservoir outlet aperture fluidly connects the wash buffer reservoir and the membrane compartment, and wherein the membrane compartment comprises a flange surrounding the outlet aperture, and wherein the flange is configured to retain wash buffer solution in the membrane compartment so the membrane does not dry out during washing.

2. The apparatus of claim 1 , wherein the membrane compartment is configured to hold a plurality of membranes.

3. The apparatus of claim 1 , wherein a diameter of the wash buffer reservoir outlet aperture is selected to allow wash buffer solution to drip through the wash buffer reservoir outlet aperture from the wash buffer reservoir to the membrane compartment at a predetermined rate.

4. The apparatus of claim 1 , wherein a diameter of the membrane compartment outlet aperture is selected to allow fluid to drip through the membrane compartment outlet aperture from the membrane compartment to the waste compartment at a predetermined rate.

5. The apparatus of claim 1 , further comprising a means for changing a diameter of the membrane compartment outlet aperture.

6. The apparatus of claim 1 , further comprising a means for changing a diameter of the wash buffer reservoir outlet aperture.

7. The apparatus of claim 1 , further comprising an agitator, wherein the waste compartment is on the agitator, and wherein the agitator is configured to agitate the wash buffer solution relative to the membrane compartment.

8. The apparatus of claim 7 , wherein the agitator comprises a platform that is configured to at least one of rock or rotate the waste compartment, the membrane compartment, and the wash buffer reservoir.

9. The apparatus of claim 7 , wherein the agitator is integral with the waste compartment.

10. The apparatus of claim 1 , wherein the wash buffer reservoir, the membrane compartment, and the waste compartment are arranged so that gravity contributes to flow of wash buffer solution from the wash buffer reservoir to the membrane compartment, and from the membrane compartment to the waste compartment.

11. A method comprising:
placing at least one membrane in a membrane compartment;
disposing the membrane compartment on a waste compartment;
disposing a wash buffer reservoir on the membrane compartment;
at least partially filling the wash buffer reservoir with a wash buffer solution; and
agitating, with an agitator, the membrane compartment, the waste compartment, and the wash buffer reservoir to cause at least some wash buffer solution in the wash buffer reservoir to flow through a wash buffer reservoir outlet aperture into the membrane compartment to wash the at least one membrane, and to cause at least some wash buffer solution in the membrane compartment to flow through a membrane compartment outlet aperture to the waste compartment, and wherein the membrane compartment comprises a flange surrounding the outlet aperture, and wherein the flange is configured to retain wash buffer solution in the membrane compartment so the membrane does not dry out during washing.

12. The method of claim 11 , wherein the membrane compartment is configured to hold a plurality of membranes.

13. The method of claim 11 , wherein a diameter of the wash buffer reservoir outlet aperture is selected to allow fluid to drip through the wash buffer reservoir outlet aperture from the wash buffer reservoir to the membrane compartment at a predetermined rate.

14. The method of claim 11 , wherein a diameter of the membrane compartment outlet aperture is selected to allow fluid to drip through the membrane compartment outlet aperture from the membrane compartment to the waste compartment at a predetermined rate.

15. The method of claim 11 , further comprising changing a diameter of the membrane compartment outlet aperture to establish a selected flow rate of wash buffer solution through the membrane compartment outlet aperture.

16. The method of claim 11 , further comprising changing a diameter of the wash buffer reservoir outlet aperture to establish a selected flow rate of wash buffer solution through the wash buffer reservoir outlet aperture.

17. The method of claim 11 , wherein the agitator comprises a platform that is configured to at least one of rock or rotate the waste compartment, the membrane compartment, and the wash buffer reservoir.

18. An apparatus comprising:
a waste compartment;
a membrane compartment on the waste compartment, wherein the membrane compartment comprises a membrane compartment outlet aperture and is configured to hold at least one membrane, wherein the membrane compartment outlet aperture fluidly connects the membrane compartment and the waste compartment, and wherein the membrane compartment comprises a means for changing a diameter of the membrane compartment outlet aperture; and
a wash buffer reservoir on the membrane compartment, wherein the wash buffer reservoir is configured to contain wash buffer solution, wherein the wash buffer reservoir comprises a wash buffer reservoir outlet aperture, and wherein the wash buffer reservoir outlet aperture fluidly connects the wash buffer reservoir and the membrane compartment, wherein the wash buffer reservoir comprises a means for changing a diameter of the wash buffer reservoir outlet aperture, and wherein the membrane compartment comprises a flange surrounding the outlet aperture, and wherein the flange is configured to retain wash buffer solution in the membrane compartment so the membrane does not dry out during washing.

19. The apparatus of claim 3 , wherein the wash buffer reservoir is configured to hold a volume of wash buffer solution sufficient to allow wash buffer solution to flow through the wash buffer reservoir outlet aperture to the membrane compartment at the predetermined rate for a duration of washing of the at least one membrane.

20. The apparatus of claim 4 , wherein the wash buffer reservoir is configured to hold a volume of wash buffer solution sufficient to allow wash buffer solution to flow through the membrane compartment outlet aperture to the waste compartment at the predetermined rate for a duration of washing of the at least one membrane.

Assignees

• Univ Minnesota

Inventors

• Anderson Brett
• Harris Reuben

Classifications

• G01N27/44704 Primary

  Details; Accessories · CPC title

• B08B3/044 Primary

  using agitated containers in which the liquid and articles or material are placed (by means of ultrasonic vibrations B08B3/12; cleaning casks or barrels by agitating B08B9/0817; mixers with shaking, oscillating or vibrating mechanisms B01F31/00) · CPC title

• G01N27/44739

  Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots · CPC title

• B01D41/04

  of rigid self-supporting filtering material · CPC title