Compositions and methods for producing starch with novel functionality

Patent metadata

Field	Value
Publication number	US-10669553-B2
Application number	US-201815963885-A
Country	US
Kind code	B2
Filing date	Apr 26, 2018
Priority date	Apr 5, 2016
Publication date	Jun 2, 2020
Grant date	Jun 2, 2020

Abstract

This invention disclosure relates to novel maize starch. The starch can be made from the newly developed waxy sugary-2 double-mutant maize that has low activity of Granule Bound Starch Synthase I (GBSSI), which results in low amylose level. The starch from newly developed waxy sugary-2 double-mutant is freeze-thaw stable and has high viscosity. In comparison with the starch of the existing waxy sugary-2 double-mutant maize, the new waxy sugary-2 double-mutant maize starch showed, inter alia, improved pasting profile, starch granule integrity, larger starch granule size, and higher viscosity.

First claim

What is claimed is:

1. A double-mutant corn plant, wherein the corn plant is homozygous recessive for a starch synthase IIa (su2) gene, and either homozygous or heterozygous for a mutated Granule-bound starch synthase I (GBSSI) gene, wherein the double-mutant corn plant has a phenotype in which the mutated GBSSI gene has less GBSSI activity than a wild type GBSSI gene (Wx), but has higher GBSSI activity than a recessive, loss-of-function GBSSI gene (wx).

2. The double-mutant corn plant of claim 1 , wherein the mutated GBSSI gene is the wx-Stonor (wxS) allele.

3. The double-mutant corn plant of claim 1 , wherein the plant is generated by cross pollination of a first corn plant having a genotype of wxwxsu2su2, and a second corn plant having a genotype of wxSwxSsu2su2 or a genotype of wxSwxsu2su2.

4. The double-mutant corn plant of claim 1 , producing a corn seed having an endosperm wherein the endosperm of the corn seed has one dose, two doses, or three doses of a wxS allele, and wherein the endosperm has a genotype of wxSwxwxsu2su2su2, wxSwxSwxsu2su2su2, or wxSwxSwxSsu2su2su2.

5. The double-mutant corn plant of claim 1 , producing a corn seed comprising an endosperm having starch wherein the starch produced in the endosperm of the corn plant has a higher pasting temperature compared to a starch produced in an endosperm of a double, waxy sugary-2 recessive mutant corn plant having a genotype of wxwxwxsu2su2su2.

6. A seed of the double-mutant corn plant of claim 1 .

7. A plant part, plant cell, or tissue culture of the double-mutant corn plant of claim 1 .

8. The plant part of claim 7 , wherein the plant part is selected from the group consisting of an endosperm, a leaf, a flower, an ovule, a pollen, a rootstock, and a scion.

9. A method for producing starch comprising obtaining starch from an endosperm of a corn seed wherein the corn seed is from double-mutant corn plant of claim 1 .

10. A method for producing starch comprising growing the double-mutant corn plant of claim 1 , and harvesting seeds from the double-mutant corn plant.

11. A method for producing a corn seed comprising crossing a first parent corn plant with a second parent corn plant and harvesting the resultant corn seed, wherein said first parent corn plant and/or second parent corn plant is the double-mutant corn plant of claim 1 .

12. The method of claim 11 wherein producing a corn seed comprises self-pollinating the double-mutant corn plant of claim 1 and harvesting the resultant corn seed.

13. A method of vegetatively propagating the corn plant of claim 1 , said method comprising collecting a part of the plant of claim 1 and regenerating a plant from said part.

14. A method of producing a corn plant derived from the double-mutant corn plant of claim 1 , the method comprising self-pollinating the double-mutant plant of claim 1 at least once to produce a progeny plant derived from the double-mutant plant of claim 1 .

15. A method of producing a corn plant derived from the double-mutant plant of claim 1 , the method comprising crossing the double-mutant plant of claim 1 with a second corn plant to produce a progeny plant of the double-mutant plant of claim 1 .

16. A method of producing a corn plant having the phenotype of the double-mutant plant of claim 1 comprising obtaining one of a first plant, plant part, plant cell, or tissue culture and introducing a transgene into the first plant or plant, plant cell or tissue culture to obtain a modified plant material, and using the modified plant material to obtain the corn plant having the phenotype of the double-mutant plant of claim 1 , wherein the transgene confers the phenotype of the double-mutant plant of claim 1 to the corn plant.

17. A method of producing a corn plant having the phenotype of the double-mutant plant of claim 1 comprising obtaining one of a first plant, plant part, plant cell, or tissue culture and gene-editing the first plant, plant part, plant cell, or tissue culture to obtain a modified plant material, and using the modified plant material to obtain the corn plant having the phenotype of the double-mutant plant of claim 1 wherein the edited gene confers the phenotype of the double-mutant plant of claim 1 to the corn plant.

18. A method of producing a corn plant having the phenotype of the double-mutant plant of claim 1 comprising obtaining one of a first plant, plant part, plant cell, or tissue culture and introducing a mutation into the first plant, plant part, plant cell, or tissue culture the mutation to obtain a modified plant material comprising a mutated genetic sequence, and using the modified plant material to obtain the corn plant having the phenotype of the double-mutant plan of claim 1 , wherein the genetic sequence confers the phenotype of the double-mutant plant of claim 1 to the corn plant.

19. A starch extracted from a double mutant corn plant, wherein the double-mutant corn plant is the corn plant of claim 1 .

20. The starch of claim 19 wherein the double-mutant corn plant is the corn plant of claim 3 .

21. The starch of claim 19 wherein the double-mutant corn plant is the corn plant of claim 4 , and wherein the starch is extracted from the endosperm of a seed produced by the double-mutant corn plant of claim 4 .

Assignees

• Corn Products Dev Inc

Inventors

• Jiang Hongxin
• Ostrander Brad
• Lane Chris

Classifications

• C08B30/044 Primary

  from corn or maize · CPC title

• C12N9/1051

  Hexosyltransferases (2.4.1) · CPC title

• A01H5/10

  Seeds · CPC title

• C12Y204/01021

  Starch synthase (2.4.1.21) · CPC title

• A01H1/04

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title