Device, use of said device, and method for separating substances with an improved utilization of the capacity of chromatographic media

Patent metadata

Field	Value
Publication number	US-10669307-B2
Application number	US-201515323778-A
Country	US
Kind code	B2
Filing date	May 12, 2015
Priority date	Jul 10, 2014
Publication date	Jun 2, 2020
Grant date	Jun 2, 2020

Abstract

The present invention relates to a device for separating and/or isolating substances in or from a mixture with improved utilization of the capacity of chromatographic media, the device comprising a first chromatography system, a second chromatography matrix downstream of the first chromatography system, and a sensor for detecting the substances present in the fluid. Furthermore, the present invention relates to both the use of said device and a method for separating and/or isolating substances in or from a mixture in a fluid.

First claim

The invention claimed is:

1. A device for separating and/or isolating substances in or from a mixture in a fluid, consisting of a first chromatography system which comprises one or more chromatography matrices, a single sensor downstream of the first chromatography system for detecting the substances present in the fluid, and a second chromatography matrix downstream of the single sensor, the second chromatography matrix being connected downstream of the first chromatography system such that the fluid leaving the first chromatography system is guidable through the second chromatography matrix wherein the single sensor for detecting the substances present in the fluid is interposed between the first chromatography system and the second chromatography matrix and determines on a basis of a change in a slope of a breakthrough signal an overloading of the first chromatography system, and wherein said single sensor for detecting the substances present in the fluid is the only sensor comprised in the device, and wherein the combination of the first chromatography system, the second chromatography matrix and the single sensor is present in a unified body.

2. The device as claimed in claim 1 , wherein the second chromatography matrix is a convective chromatography matrix such as a membrane adsorber or a monolith.

3. The device as claimed in claim 1 , wherein a measurement principle of the sensor is based on UV or IR absorption spectroscopy, terahertz spectroscopy, manometry, conductometry, potentiometry, refractometry, radiometry, fluorescence spectroscopy especially of surface fluorescence quenching, ATR (attenuated total reflection) infrared spectroscopy especially of surface plasmon resonance spectroscopy (SPRS), potentiometry, polarimetry, impendance spectroscopy, NMR spectroscopy, Raman spectroscopy, turbidimetry, nephelometry and on ultrasound transit time, or comprises a combination of multiple techniques as detection principle for the sensor.

4. The device as claimed in claim 1 , wherein the first chromatography system has a ligand and the second chromatography matrix has a ligand, the ligand of the first chromatography system and the ligand of the second chromatography matrix each interacting with at least one substance in the mixture via an identical chemical and/or physical interaction, selected from ion exchangers, salt-tolerant ligands, chelating agents, thiophilic or hydrophobic ligands of various chain lengths and configurations, “reversed-phase” ligands, reactive dyes and other dyes, of the inorganic molecules and ions, organic and inorganic compounds thereof, affinity ligands, high-molecular-weight ligands, enzymes and subunits and also parts thereof, structural proteins, receptors and effectors and also parts thereof, viruses and parts thereof, xenobiotics, pharmaceuticals and active pharmaceutical ingredients, alkaloids, antibiotics, biomimetics and of the catalysts.

5. The device as claimed in claim 1 , wherein the first chromatography system and the second chromatography matrix have at least one identical ligand which interacts with at least one substance in the mixture via at least one chemical and/or physical interaction, selected from ion exchangers, salt tolerant ligands, chelating agents, thiophilic or hydrophobic ligands of various chain lengths and configurations, “reversed-phase” ligands, reactive dyes and other dyes, of the inorganic molecules and ions, organic and inorganic compounds thereof, affinity ligands, high-molecular-weight ligands, enzymes and subunits and also parts thereof, structural proteins, receptors and effectors and also parts thereof, viruses and parts thereof, xenobiotics, pharmaceuticals and active pharmaceutical ingredients, alkaloids, antibodies, biometrics and of the cataylysts.

6. The device as claimed in claim 1 , wherein the first chromatography system and the second chromatography matrix each have at least one different ligand which interacts with at least one substance in the mixture via at least one chemical and/or physical interaction, selected from ion exchangers, salt-tolerant ligands, chelating agents, thiophilic or hydrophobic ligands of various chain lengths and configurations, “reversed-phase” ligands, reactive dyes and other dyes, of the inorganic molecules and ions, organic and inorganic compounds thereof, affinity ligands, high-molecular-weight ligands, enzymes and subunits and also parts thereof, structural proteins, receptors and effectors and also parts thereof, viruses and parts thereof, xenobiotics, pharmaceuticals and active pharmaceutical ingredients, alkaloids, antibiotics, biomimetics and of the catalysts.

7. The device as claimed in claim 1 , wherein the fluid leaving the first chromatography system is completely guidable through the second chromatography matrix.

8. The device as claimed in claim 1 , wherein a capacity of the second chromatography matrix is smaller than or identical to a capacity of the first chromatography system.

9. The device as claimed in claim 1 , wherein the sensor is nondestructive with regard to the target molecule.

10. The device as claimed in claim 1 , wherein the sensor is a UV, IR, pH, pressure or conductivity sensor.

11. A method for separating and/or isolating substances in or from a mixture in a fluid, comprising the steps of guiding the fluid through the device as claimed in claim 1 and collecting the fluid which has been guided through.

12. The method as claimed in claim 11 , wherein the mixture is a solution of a synthetically or biologically produced product, or a natural substance.

13. The method as claimed in claim 11 , wherein the mixture is a protein-containing solution.

14. The method as claimed in claim 11 , wherein the mixture is an antibody-containing solution.

15. The use of the device as claimed in claim 1 for separating and/or isolating a target substance in or from a mixture in a fluid.

16. The use of the device as claimed in claim 1 , wherein the use of the device allows an improvement in the utilization of the capacity of the first chromatography system.

17. The use of the device as claimed in claim 1 , wherein the use of the device allows an over 60% utilization of the capacity of the first chromatography system.

Assignees

• Sartorius Stedim Biotech Gmbh

Inventors

• Villain Louis
• Mittelstät Jörg
• Santos De Matos Fortuna Ana Raquel
• Töppner Katrin

Classifications

• B01D15/3809

  of the antigen-antibody type, e.g. protein A, G or L chromatography · CPC title

• B01D15/363

  Anion-exchange · CPC title

• C07K1/20

  Partition-, reverse-phase or hydrophobic interaction chromatography · CPC title

• B01D15/1871

  placed in series · CPC title

• G01N2030/8831

  involving peptides or proteins · CPC title