Method of increasing satellite cell proliferation with an HDAC inhibitor

Patent metadata

Field	Value
Publication number	US-10660902-B2
Application number	US-201715728476-A
Country	US
Kind code	B2
Filing date	Oct 9, 2017
Priority date	Jun 16, 2011
Publication date	May 26, 2020
Grant date	May 26, 2020

Abstract

The invention provides methods for inducing, enhancing or increasing satellite cell proliferation, and an assay for screening for a candidate compound for inducing, enhancing or increasing satellite cell proliferation. Also provided are methods for repairing or regenerating a damaged muscle tissue of a subject.

First claim

What is claimed is:

1. A method of increasing satellite cell proliferation, the method comprising: contacting a satellite cell with a benzamide, or a hydroxamic acid selected from the group consisting of suberoylanilide hydroxamic acid (SAHA), trichostatin A (TSA), trichostatin C (TSC), salicylhydroxamic acid, oxamflatin, suberic bishydroxamic acid (SBHA), m-carboxycinnamic acid bishydroxamic acid (CBHA), pyroxamide (CAS RN 382180-17-8), diethyl bis-(pentamethylene-N,Ndimethylcarboxamide)malonate (EMBA), azelaic bishydroxamic acid (ABHA), azelaic-1-hydroxamate-9-anilide (AAHA), 6-(3-Chlorophenylureido) carpoic hydroxamic acid, and A-161906, in an amount effective to increase satellite cell proliferation.

2. The method of claim 1 , wherein the contacting is in vivo.

3. The method of claim 2 , wherein the in vivo contacting is in a human.

4. The method of claim 2 , wherein the in vivo contacting is in a subject, wherein the subject is in need of treatment for damaged muscle tissue.

5. The method of claim 4 , wherein the damaged muscle tissue is the result of a physical injury or accident, disease, infection, over-use, loss of blood circulation, or muscle atrophy or wasting.

6. The method of claim 4 , wherein the damaged muscle tissue is the result of muscle atrophy/wasting.

7. A method for muscle repair or regeneration in a subject having damaged muscle tissue, the method comprising administering to the subject a benzamide, or a hydroxamic acid selected from the group consisting of suberoylanilide hydroxamic acid (SAHA), trichostatin A (TSA), trichostatin C (TSC), salicylhydroxamic acid, oxamflatin, suberic bishydroxamic acid (SBHA), m-carboxycinnamic acid bishydroxamic acid (CBHA), pyroxamide (CAS RN 382180-17-8), diethyl bis-(pentamethylene-N,Ndimethylcarboxamide)malonate (EMBA), azelaic bishydroxamic acid (ABHA), azelaic-1-hydroxamate-9-anilide (AAHA), 6-(3-Chlorophenylureido) carpoic hydroxamic acid, and A-161906, in an amount effective to increase satellite cell proliferation.

8. The method of claim 7 , wherein the subject is human.

9. The method of claim 7 , wherein the damaged muscle tissue is the result of a physical injury or accident, disease, infection, over-use, loss of blood circulation, or muscle atrophy or wasting.

10. The method of claim 9 , wherein the damaged muscle tissue is the result of muscle atrophy/wasting.

Assignees

• Harvard College

Inventors

• Rubin Lee L
• Gee Amanda
• Wagers Amy J

Classifications

• A61P21/00

  Drugs for disorders of the muscular or neuromuscular system · CPC title

• A61P21/06

  Anabolic agents (androgens A61P5/26) · CPC title

• A61K31/4164

  1,3-Diazoles · CPC title

• A61K31/16

  Amides, e.g. hydroxamic acids · CPC title

• C07C259/04

  without replacement of the other oxygen atom of the carboxyl group, e.g. hydroxamic acids · CPC title