Method and apparatus for reducing carryover of reagents and samples in analytical testing

Patent metadata

Field	Value
Publication number	US-10656169-B2
Application number	US-201515311120-A
Country	US
Kind code	B2
Filing date	May 26, 2015
Priority date	May 29, 2014
Publication date	May 19, 2020
Grant date	May 19, 2020

Abstract

A method of reducing carryover of liquids, such as reagent and/or samples, in analytical or immunoassay testing. The method includes providing an incubation member including a plurality of reaction vessels thereon, pre-assigning certain ones of the reaction vessels in the incubation member for only first test types or certain reagent types for all test lots; and pre-assigning other ones of a second subset of reaction vessels in the incubation member for only second test types for all test lots. By ensuring no mixing of potentially interfering test types or reagent types from test lot to test lot, sample and/or reagent carryover is mitigated. Testing apparatus adapted to carry out the methods are provided, as are other aspects.

First claim

What is claimed is:

1. A method of reducing carryover of reagents in analytical testing, comprising:
providing an incubation member including receptacles including a first subset of reaction vessels and a second subset of reaction vessels;
providing a dispensing system for dispensing reagents into the first subset of reaction vessels and into the second subset of reaction vessels;
providing a controller in communication with the dispensing system;
defining a first reagent type comprising one or more non-interfering reagents that do not act as an analyte to any tests that include reagents defined within the first reagent type;
defining a second reagent type comprising one or more non-interfering reagents that do not act as an analyte to any tests that include reagents defined within the second reagent type, the reagents included within the second reagent type acting as an analyte to any tests that include reagents defined within the first reagent type;
first reagent dispensing by the dispensing system under control of the controller of a reagent defined as the first reagent type only into reaction vessels within the first subset for tests of a first test type that include addition of the first reagent type; and
second reagent dispensing by the dispensing system under control of the controller of a reagent defined as the second reagent type only into reaction vessels within the second subset for tests of a second test type that include the addition of the second reagent type;
first sample dispensing of samples into the first subset of reaction vessels and into the second subset of reaction vessels;
performing first tests of the first test type in the first subset of reaction vessels and second tests of the second test type in the second subset of reaction vessels;
second sample dispensing of samples into the first subset of reaction vessels and the second subset of reaction vessels after performing the first tests and the second tests;
third reagent dispensing of a reagent defined as the first reagent type only into the first subset of reaction vessels after performing the first tests;
fourth reagent dispensing of a reagent defined as the second reagent type only into the second subset of reaction vessels after performing the second tests; and
performing tests of a third test type in the first subset of reaction vessels and performing tests of a fourth test type in the second subset of reaction vessels subsequent to the second sample dispensing.

2. The method of claim 1 , comprising:
pre-assigning certain test types in accordance with a pattern of the receptacles of the incubation member.

3. A testing apparatus, comprising:
an incubation member including reaction vessels thereon;
a controller configured to execute a selection routine in order to:
pre-assign a first subset of the reaction vessels on the incubation member to carry out all tests of a first test type including a first reagent type, the first reagent type including one or more reagents that do not act as an analyte to any tests that include reagents defined within the first reagent type; and
pre-assign a second subset of the reaction vessels to carry out all tests of a second test type including a second reagent type, the second reagent type-including one or more reagents that do not act as an analyte to any tests that include reagents defined within the second reagent type;

a drive system operable to move the incubation member responsive to the selection routine;
a dispensing system configured to perform
first sample dispensing of samples into the first subset of the reaction vessels and the second subset of the reaction vessels,
first reagent dispensing of a reagent of the first reagent type only in the first subset of the reaction vessels for tests of a first test type,
second reagent dispensing of a reagent of the second reagent type only in the second subset of the reaction vessels for tests of a second test type,
second sample dispensing of samples into the first subset of the reaction vessels and the second subset of the reaction vessels after tests of the first test type are performed in the first subset of the reaction vessels and tests of the second test type are performed in the second subset of the reaction vessels,
third reagent dispensing of a reagent defined as the first reagent type only into the first subset of the reaction vessels for tests of a third test type, and
fourth reagent dispensing of a reagent defined as the second reagent type only into the second subset of the reaction vessels for tests of a fourth type;

the controller further configured to perform tests of a third test type in the first subset of reaction vessels and perform tests of a fourth test type in the second subset of reaction vessels subsequent to the second sample dispensing; and
a read device configured to read a relative concentration of a component of interest within a reaction vessel in which either the first test type or the second test type has been carried out in response to the selection routine.

4. The testing apparatus of claim 3 , wherein the incubation member comprises a ring including a plurality of numbered receptacles configured to contain the reaction vessels, and wherein the first subset comprises reaction vessels in odd numbered receptacles, and the second subset comprises reaction vessels in even numbered receptacles.

5. The testing apparatus of claim 3 , wherein the incubation member comprises a ring including a plurality of receptacles configured to contain the reaction vessels, the first subset comprises reaction vessels arranged in a first pattern, and the second subset comprises reaction vessels arranged in a second pattern.

6. A method of reducing carryover of reagents in analytical testing, comprising:
providing a first subset of reaction vessels and a second subset of reaction vessels;
defining a first reagent type comprising one or more non-interfering reagents that do not act as an analyte to any tests that include reagents defined within the first reagent type;
defining a second reagent type comprising one or more non-interfering reagents that do not act as an analyte to any tests that include reagents defined within the second reagent type;
first sample dispensing of samples into the first subset of reaction vessels and into the second subset of reaction vessels;
first reagent dispensing of a reagent defined as the first reagent type only into reaction vessels within the first subset; and
second reagent dispensing of a reagent defined as the second reagent type only into reaction vessels within the second subset;
performing first tests of a first test type in the first subset of reaction vessels;
performing second tests of a second test type in the second subset of reaction vessels;
second sample dispensing of samples into the first subset of reaction vessels and the second subset of reaction vessels after performing the first tests and the second tests;
third reagent dispensing of a reagent defined as the first reagent type only into the first subset of reaction vessels after performing the first tests;
fourth reagent dispensing of a reagent defined as the second reagent type only into the second subset of reaction vessels after performing the second tests; and
performing third tests of a third test type in the first subset of reaction vessels and performing fourth tests of a fourth test type in the second subset of reaction vessels subsequent to the second sample dispensing.

Assignees

• Siemens Healthcare Diagnostics Inc

Inventors

• Wang Jianfang
• Sprenkle Mark
• Ingersoll Colin
• Frye Stephen

Classifications

• G01N2035/00356

  Holding samples at elevated temperature (incubation) · CPC title

• G01N35/0092 Primary

  Scheduling · CPC title

• G01N2035/00673

  of reagents · CPC title

• G01N2035/0094

  optimisation; experiment design · CPC title

• G01N35/00663

  of consumables · CPC title