Method for producing an amide

Patent metadata

Field	Value
Publication number	US-10655152-B2
Application number	US-201515513670-A
Country	US
Kind code	B2
Filing date	Sep 30, 2015
Priority date	Sep 30, 2014
Publication date	May 19, 2020
Grant date	May 19, 2020

Abstract

The present invention relates to means and methods for producing an amide compound from a nitrile compound with less acrylic acid as by-product using a Nitrile hydratase (NHase) and Amidase producing microorganism as biocatalyst. Also provided is an aqueous amide compound obtained by the methods of the invention as well as a composition comprising acrylamide or polyacrylamide as well as a dried microorganism exhibiting a NHase/Amidase activity ratio of at least 400 when being brought into contact with a nitrile compound to convert said nitrile compound into an amide compound.

First claim

The invention claimed is:

1. A method for producing an amide compound from a nitrile compound, the method comprising:
contacting the nitrile compound with a microorganism producing a nitrile hydratase (NHase) and an amidase, wherein the microorganism has been previously pre-treated by drying before the contacting with the nitrile compound, wherein a ratio of a NHase activity to an amidase activity of the microorganism is increased, when compared to a reference microorganism, which is not pre-treated by drying before being contacted with the nitrile compound,
wherein the microorganism is selected from the group consisting of Rhodococcus rhodochrous, Rhodococcus pyridinovorans, Rhodococcus erythropolis, Rhodococcus equi, Rhodococcus ruber , and Rhodococcus opacus,
the amide compound is at least one selected from the group consisting of acrylamide, methacrylamide, acetamide, and nicotinamide,
the nitrile compound is at least one selected from the group consisting of acrylonitrile, methacrylonitrile, acetonitrile, and 3-cyanopyridine, and
wherein the drying has been conducted by spray drying, freeze-drying, heat drying, air drying, vacuum drying, fluidized-bed drying, spray granulation, or a combination thereof.

2. The method of claim 1 , wherein the ratio of the NHase activity to the amidase activity of the microorganism is increased by a factor of at least 1.4, when compared to the reference microorganism.

3. The method of claim 1 , wherein the ratio of the amidase activity to the NHase activity of the microorganism is decreased by a factor of at least 0.7, when compared to the reference microorganism, and wherein the reference microorganism is the microorganism producing a nitrile hydratase (NHase) and an amidase that has not been pre-treated by drying.

4. The method of claim 1 , wherein the microorganism exhibits an NHase/amidase activity ratio of at least 400.

5. A method for producing an amide compound from a nitrile compound, the method comprising:
a) drying a microorganism producing a NHase and an amidase; and
b) contacting the nitrile compound with the microorganism after drying
wherein drying increases a NHase/amidase activity ratio of the microorganism, compared to a reference microorganism, which is not treated by drying, wherein the microorganism is selected from the group consisting of Rhodococcus rhodochrous, Rhodococcus pyridinovorans, Rhodococcus erythropolis, Rhodococcus equi, Rhodococcus ruber , and Rhodococcus opacus,
the amide compound is at least one selected from the group consisting of acrylamide, methacrylamide, acetamide, and nicotinamide,
the nitrile compound is at least one selected from the group consisting of acrylonitrile, methacrylonitrile acetonitrile, and 3-cyanopyridine, and
wherein the drying has been conducted by spray drying, freeze-drying, heat drying, air drying, vacuum drying, fluidized-bed drying, spray granulation, or a combination thereof.

6. The method of claim 1 , further comprising reconstituting the pre-treated microorganism after the drying, wherein the contacting is conducted with a reconstituted microorganism.

7. The method of claim 6 , wherein in the reconstituting, the pre-treated microorganism is suspended in an aqueous composition.

8. The method of claim 1 , wherein the contacting is conducted with the pre-treated microorganism in the form of a powder, granule, or suspension, and/or in the form of a matrix bound microorganism.

9. The method of claim 1 , wherein the microorganism is Rhodococcus rhodochrous or Rhodococcus pyridinovorans.

10. The method of claim 9 , wherein the microorganism is Rhodococcus rhodochrous (NCIMB 41164), Rhodococcus rhodochrous (FERM BP-1478) or Rhodococcus rhodochrous M33.

11. The method of claim 1 , wherein the nitrile compound is acrylonitrile.

12. The method of claim 1 , wherein the amide compound is acrylamide.

13. The method of claim 1 , further comprising:
activating by mixing the dried microorganism with an aqueous solution to obtain an activation mixture, wherein the activation mixture comprises a buffer, and
converting the nitrile compound to the amide compound by contacting the nitrile compound with the activation mixture in a reaction mixture, wherein the reaction mixture comprises the buffer and wherein a ratio of a molar concentration of the buffer in the activation mixture to a molar concentration of the buffer in the reaction mixture is about 2:1 or more.

14. A method for producing a microorganism with an increased NHase/amidase activity ratio, comprising:
drying a microorganism producing a NHase and an amidase, and contacting a nitrile compound with the microorganism after the step of drying,

wherein the microorganism is selected from the group consisting of Rhodococcus rhodochrous, Rhodococcus pyridinovorans, Rhodococcus erythropolis, Rhodococcus equi, Rhodococcus ruber , and Rhodococcus opacus,
and the drying is conducted by spray drying, freeze-drying, heat drying, air drying, vacuum drying, fluidized-bed drying, spray granulation, or a combination thereof.

15. A method for reducing the formation of acrylic acid when producing acrylamide from an acrylnitrile compound, the method comprising contacting acrylonitrile with a microorganism producing a NHase and an amidase obtained by the method of claim 14 .

16. The method of claim 1 , wherein the microorganism producing a NHase and an amidase is not immobilized before being dried.

17. The amide compound obtained by the method of claim 1 , wherein the amide compound is in an aqueous solution.

18. A composition comprising acrylamide or polyacrylamide and a microorganism producing a NHase and an amidase that has been pre-treated by drying, wherein the microorganism exhibits a NHase/amidase activity ratio of at least 400, and/or wherein a ratio of a NHase activity to an amidase activity is increased by a factor of at least 1.7, when compared to a reference microorganism, which is the same microorganism but has not been pretreated by drying, wherein the microorganism is selected from the group consisting of Rhodococcus rhodochrous, Rhodococcus pyridinovorans, Rhodococcus erythropolis, Rhodococcus equi, Rhodococcus ruber , and Rhodococcus opacus , and wherein the drying has been conducted by spray drying, freeze-drying, heat drying, air drying, vacuum drying, fluidized-bed drying, spray granulation, or a combination thereof.

Assignees

• Solenis Tech Lp

Inventors

• Budde Michael
• Braun Michael
• Daeuwel Juergen
• Oedman Peter
• Baldenius Kai-Uwe
• Kleiner Matthias
• Freyer Stephan

Classifications

• C12Y305/01004

  Amidase (3.5.1.4) · CPC title

• C12Y305/05001

  Nitrilase (3.5.5.1) · CPC title

• C12P13/02 Primary

  Amides, e.g. chloramphenicol {or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes (peptides C12P21/00 or C07K)} · CPC title

• C12N9/80 Primary

  acting on amide bonds in linear amides {(3.5.1)} · CPC title

• C12N9/78

  acting on carbon to nitrogen bonds other than peptide bonds (3.5) · CPC title