Peptide having anticancer activity, and pharmaceutical composition, health functional food composition and functional cosmetic composition for preventing and treating cancer comprising the same as active ingredient

Patent metadata

Field	Value
Publication number	US-10654889-B2
Application number	US-201716329139-A
Country	US
Kind code	B2
Filing date	Aug 28, 2017
Priority date	Aug 30, 2016
Publication date	May 19, 2020
Grant date	May 19, 2020

Abstract

The present invention relates to a peptide having anticancer activity, and a pharmaceutical composition, health functional food composition and functional cosmetic composition for preventing and treating cancer including the same as an active ingredient, and more particularly, to a peptide set forth in SEQ ID NO: 1, wherein the peptide binds to a transcription factor CP2c and has prophylactic and therapeutic activities against cancer, and a pharmaceutical composition, a health functional food composition and a functional cosmetic composition for preventing and treating cancer including the same as an active ingredient.
<SEQ ID NO: 1> Tyr-Pro-Gln-Arg.

First claim

The invention claimed is:

1. A peptide comprising the following SEQ ID NOs: 1 and 2:

<SEQ ID NO: 1>

Tyr-Pro-Gln-Arg

<SEQ ID NO: 2>

Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys

wherein the peptide set forth in SEQ ID NO: 2 is bound to the C-terminal Arg of the peptide set forth in SEQ ID NO: 1.

2. The peptide of claim 1 , further comprising an acetyl and an amide group bound to the N-terminal Tyr of the peptide set forth in SEQ ID NO: 1 and C-terminal Cys of the peptide set forth in SEQ ID NO: 2, respectively.

3. The peptide of claim 1 , further comprising a peptide set forth in the following SEQ ID NO: 3 bound to the N-terminal Tyr of the peptide set forth in SEQ ID NO: 1, and fluorescein isothiocyanate (FITC) bound to N-terminal Lys of the peptide set forth in the following SEQ ID NO: 3:

<SEQ ID NO: 3>

Lys-Cys-Lys-Gly-Gly-Ser-Gly-Gly-Ser

(provided that each of Lys 1 and 3 residues

of the sequence is 6-aminohexanoic acid.).

4. The peptide of claim 3 , further comprising an amide group bound to the C-terminal Cys of the peptide set forth in SEQ ID NO: 2.

5. A peptide comprising the following SEQ ID NOs: 1 and 4:

<SEQ ID NO: 1>

Tyr-Pro-Gln-Arg

<SEQ ID NO: 4>

Arg-Gly-Asp.

wherein the peptide set forth in SEQ ID NO: 4 is bound to the C-terminal Arg of the peptide set forth in SEQ ID NO: 1.

6. The peptide of claim 5 , further comprising an acetyl and an amide group bound to the N-terminal Tyr of the peptide set forth in SEQ ID NO: 1 and C-terminal Asp of the peptide set forth in SEQ ID NO: 4, respectively.

7. The peptide of claim 5 , wherein a cyclic peptide is formed via a direct peptide bond between the amino group of the N-terminal Tyr of the peptide set forth in SEQ ID NO: 1 and the carboxyl group of the C-terminal Asp of the peptide set forth in SEQ ID NO: 4.

8. A peptide comprising the following SEQ ID NOs: 1 and 5:

<SEQ ID NO: 1>

Tyr-Pro-Gln-Arg

<SEQ ID NO: 5>

Lys-Ile-Lys-Lys-Val-Lys-Lys-Lys-Gly-Arg-Lys-Gly-

Ser-Lys-Ile-Lys-Lys-Val-Lys-Lys-Lys-Gly-Arg-Lys-

Gly-Gly

wherein the peptide set forth in SEQ ID NO: 5 is bound to the N-terminal Tyr of the peptide set forth in SEQ ID NO: 1.

9. The peptide of claim 8 , further comprising an acetyl and an amide group bound to N-terminal Lys of the peptide set forth in SEQ ID NO: 5 and the C-terminal Arg of the peptide set forth in SEQ ID NO: 1, respectively.

10. The peptide of claim 1 , further comprising a biotin-labeled Lys bound to ε-NH 2 of the C-terminal Cys of the peptide set forth in SEQ ID NO: 2.

11. The peptide of claim 10 , further comprising an acetyl and an amide group bound to the N-terminal Tyr of the peptide set forth in SEQ ID NO: 1 and the biotin-labeled C-terminal Lys bound to the ε-NH 2 of the C-terminal Cys of the peptide set forth in SEQ ID NO: 2, respectively.

12. A pharmaceutical composition for preventing and treating cancer comprising the peptide as in claims 1 , 5 or 8 as an active ingredient.

13. A health functional food composition for preventing and treating cancer comprising the peptide as in claims 1 , 5 or 8 as an active ingredient.

14. A functional cosmetic composition comprising the peptide as in claim 1 , 5 or 8 as an active ingredient.

Assignees

• Univ Hanyang Ind Univ Coop Found

Inventors

• Kim Chul Geun
• Kim Chan Gil
• Kim Minyoung
• Sim Hongnam

Classifications

• A61K2800/10

  General cosmetic use · CPC title

• A23L33/18

  Peptides; Protein hydrolysates · CPC title

• A23V2200/308

  having an effect on cancer prevention · CPC title

• A61K38/07

  Tetrapeptides · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title